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Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro

Interferons (IFNs) play a major role in the host’s antiviral innate immunity. In response to viral infection, IFNs bind their receptors and initiate a signaling cascade, leading to the accurate transcriptional regulation of hundreds of IFN-stimulated genes (ISGs). Porcine rotavirus (PoRV) belongs to...

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Autores principales: Deng, Lishuang, Yin, Yue, Xu, Zhiwen, Li, Fengqin, Zhao, Jun, Deng, Huidan, Jian, Zhijie, Lai, Siyuan, Sun, Xiangang, Zhu, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321941/
https://www.ncbi.nlm.nih.gov/pubmed/35889447
http://dx.doi.org/10.3390/molecules27144575
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author Deng, Lishuang
Yin, Yue
Xu, Zhiwen
Li, Fengqin
Zhao, Jun
Deng, Huidan
Jian, Zhijie
Lai, Siyuan
Sun, Xiangang
Zhu, Ling
author_facet Deng, Lishuang
Yin, Yue
Xu, Zhiwen
Li, Fengqin
Zhao, Jun
Deng, Huidan
Jian, Zhijie
Lai, Siyuan
Sun, Xiangang
Zhu, Ling
author_sort Deng, Lishuang
collection PubMed
description Interferons (IFNs) play a major role in the host’s antiviral innate immunity. In response to viral infection, IFNs bind their receptors and initiate a signaling cascade, leading to the accurate transcriptional regulation of hundreds of IFN-stimulated genes (ISGs). Porcine rotavirus (PoRV) belongs to genus Rotavirus of the Reoviridae family; the infection is a global epidemic disease and a major threat to the pig industry. In this study, we found that IFN-λ3 inhibited the replication of PoRV in both MA104 cells and IPEC-J2 cells, and this inhibition was dose-dependent. Furthermore, the antiviral activity of IFN-λ3 was more potent in IPEC-J2 cells than in MA104 cells. Further research showed that IFN-λ3 and IFN-α might inhibit PoRV infection by activating ISGs, i.e., MxA, OASL and ISG15, in IPEC-J2 cells. However, the co-treatment of IFN-λ3 and IFN-α did not enhance the antiviral activity. Our data demonstrated that IFN-λ3 had antiviral activity against PoRV and may serve as a useful antiviral candidate against PoRV, as well as other viruses in swine.
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spelling pubmed-93219412022-07-27 Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro Deng, Lishuang Yin, Yue Xu, Zhiwen Li, Fengqin Zhao, Jun Deng, Huidan Jian, Zhijie Lai, Siyuan Sun, Xiangang Zhu, Ling Molecules Brief Report Interferons (IFNs) play a major role in the host’s antiviral innate immunity. In response to viral infection, IFNs bind their receptors and initiate a signaling cascade, leading to the accurate transcriptional regulation of hundreds of IFN-stimulated genes (ISGs). Porcine rotavirus (PoRV) belongs to genus Rotavirus of the Reoviridae family; the infection is a global epidemic disease and a major threat to the pig industry. In this study, we found that IFN-λ3 inhibited the replication of PoRV in both MA104 cells and IPEC-J2 cells, and this inhibition was dose-dependent. Furthermore, the antiviral activity of IFN-λ3 was more potent in IPEC-J2 cells than in MA104 cells. Further research showed that IFN-λ3 and IFN-α might inhibit PoRV infection by activating ISGs, i.e., MxA, OASL and ISG15, in IPEC-J2 cells. However, the co-treatment of IFN-λ3 and IFN-α did not enhance the antiviral activity. Our data demonstrated that IFN-λ3 had antiviral activity against PoRV and may serve as a useful antiviral candidate against PoRV, as well as other viruses in swine. MDPI 2022-07-18 /pmc/articles/PMC9321941/ /pubmed/35889447 http://dx.doi.org/10.3390/molecules27144575 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Deng, Lishuang
Yin, Yue
Xu, Zhiwen
Li, Fengqin
Zhao, Jun
Deng, Huidan
Jian, Zhijie
Lai, Siyuan
Sun, Xiangang
Zhu, Ling
Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro
title Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro
title_full Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro
title_fullStr Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro
title_full_unstemmed Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro
title_short Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro
title_sort antiviral activity of porcine ifn-λ3 and ifn-α against porcine rotavirus in vitro
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321941/
https://www.ncbi.nlm.nih.gov/pubmed/35889447
http://dx.doi.org/10.3390/molecules27144575
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