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Efficient Method for Generating Point Mutations in the Vaccinia Virus Genome Using CRISPR/Cas9

The vaccinia virus (VACV) was previously used as a vaccine for smallpox eradication. Nowadays, recombinant VACVs are developed as vaccine platforms for infectious disease prevention and cancer treatment. The conventional method for genome editing of the VACV is based on homologous recombination, whi...

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Detalles Bibliográficos
Autores principales:	Boutin, Laetitia, Mosca, Estelle, Iseni, Frédéric
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321979/ https://www.ncbi.nlm.nih.gov/pubmed/35891539 http://dx.doi.org/10.3390/v14071559

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