Vast Gene Flow among the Spanish Populations of the Pest Bactrocera oleae (Diptera, Tephritidae), Phylogeography of a Metapopulation to Be Controlled and Its Mediterranean Genetic Context

SIMPLE SUMMARY: The output of olive industry at the Mediterranean Basin, headed by Spain, is huge worldwide. The olive fruit fly Bactrocera oleae is the major pest of olive orchards. The damages it causes become in considerable economic losses as well as a decrease in oil quantity and quality. A key question for the success of pest control strategies is the further knowledge about the species, and genetic data becomes essential for this purpose. The present work analyses more than 250 fruit flies from six different Mediterranean countries, showing relevant data about the genetic structure and gene flow of this damaging pest. These findings are helpful to improve the integrated pest management strategies according to the current European Guidelines. ABSTRACT: Spain is the leading producer of olives and olive oil. Ninety-five percent of world production originate from Spain and other regions of the Mediterranean Basin. However, these olive-growing countries face a major problem, the harmful fly Bactrocera oleae, the main pest of olive crops. To improve its control, one of the challenges is the further knowledge of the species and populations dynamics in this area. A phylogeographic work is necessary to further characterise the levels and distribution patterns of genetic diversity of the Spanish populations and their genetic relationships with other Mediterranean populations. A 1151 bp fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene has been analysed in over 250 specimens of the six main Mediterranean countries via sequencing. Genetic diversity parameters were high; 51 new haplotypes have been identified showing a geographical pattern across the Mediterranean area. The data revealed that olive fruit fly populations have been long time established in the Mediterranean Basin with two genetic groups. Gene flow seems to be the main process in shaping this genetic structure as well as fly’s colonisation routes that have paralleled those of the olive tree.