Cargando…

Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS

A multiresidue analytical method was developed for grapes for the following 24 plant growth regulators: 1-naphthylacetamide, 2,3,5-triiodobenzoic acid, 2,4,5-T, 2-naphthoxyacetic acid, 3-indolylacetic acid, 4-(3-indolyl)-butyric acid, 4-chlorophenoxyacetic acid, 4-nitrophenol, 6-benzylaminopurine, N...

Descripción completa

Detalles Bibliográficos
Autores principales: Pu, Chieh-Han, Lin, Shao-Kai, Chuang, Wei-Chen, Shyu, Tsyr-Horng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322231/
https://www.ncbi.nlm.nih.gov/pubmed/29567233
http://dx.doi.org/10.1016/j.jfda.2017.08.001
_version_ 1784756247755489280
author Pu, Chieh-Han
Lin, Shao-Kai
Chuang, Wei-Chen
Shyu, Tsyr-Horng
author_facet Pu, Chieh-Han
Lin, Shao-Kai
Chuang, Wei-Chen
Shyu, Tsyr-Horng
author_sort Pu, Chieh-Han
collection PubMed
description A multiresidue analytical method was developed for grapes for the following 24 plant growth regulators: 1-naphthylacetamide, 2,3,5-triiodobenzoic acid, 2,4,5-T, 2-naphthoxyacetic acid, 3-indolylacetic acid, 4-(3-indolyl)-butyric acid, 4-chlorophenoxyacetic acid, 4-nitrophenol, 6-benzylaminopurine, N6-isopentenyladenine, butralin, chlormequat chloride, chlorphonim-Cl, cloprop, forchlorfenuron, gibberellic acid 3, gibberellic acid 4, gibberellic acid 7, inabenfide, mepiquat chloride, paclobutrazol, prohydrojasmon, thidiazuron and uniconizole-P. The compounds were extracted from grape samples using an extraction method modified from the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. Liquid chromatography – tandem mass spectrometry was used for the detection and quantification of the compounds. Validation of the method was performed by using recovery studies at both intra-day and inter-day intervals, as well as by evaluation of the matrix effect, limit of quantification, trueness and precision. We used matrix-matched calibrations for the quantification of the compounds, which all resulted in determination coefficients (r(2)) higher than 0.995. The limit of quantification ranged from 0.1 to 5 ng/mL. Recovery studies using three spiking concentrations at varying levels showed recoveries of 70.2–112.6% and 67.5–101.8% at intra-day and inter-day intervals, respectively. Relative standard deviations were below 20% for the recovery studies. The extraction method were further validated by performing recovery study and matrix effect test in six different grape varieties from Taiwan and the United States and all resulted in comparable results. Application of the established method to 50 grape samples, resulted in the detection of chlormequat chloride and forchlorfenuron residues in the tested grapes. The results of the method validation and real sample analysis shows the extraction method is therefore suitable for routine monitoring of residue in grapes.
format Online
Article
Text
id pubmed-9322231
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Taiwan Food and Drug Administration
record_format MEDLINE/PubMed
spelling pubmed-93222312022-08-09 Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS Pu, Chieh-Han Lin, Shao-Kai Chuang, Wei-Chen Shyu, Tsyr-Horng J Food Drug Anal Original Article A multiresidue analytical method was developed for grapes for the following 24 plant growth regulators: 1-naphthylacetamide, 2,3,5-triiodobenzoic acid, 2,4,5-T, 2-naphthoxyacetic acid, 3-indolylacetic acid, 4-(3-indolyl)-butyric acid, 4-chlorophenoxyacetic acid, 4-nitrophenol, 6-benzylaminopurine, N6-isopentenyladenine, butralin, chlormequat chloride, chlorphonim-Cl, cloprop, forchlorfenuron, gibberellic acid 3, gibberellic acid 4, gibberellic acid 7, inabenfide, mepiquat chloride, paclobutrazol, prohydrojasmon, thidiazuron and uniconizole-P. The compounds were extracted from grape samples using an extraction method modified from the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. Liquid chromatography – tandem mass spectrometry was used for the detection and quantification of the compounds. Validation of the method was performed by using recovery studies at both intra-day and inter-day intervals, as well as by evaluation of the matrix effect, limit of quantification, trueness and precision. We used matrix-matched calibrations for the quantification of the compounds, which all resulted in determination coefficients (r(2)) higher than 0.995. The limit of quantification ranged from 0.1 to 5 ng/mL. Recovery studies using three spiking concentrations at varying levels showed recoveries of 70.2–112.6% and 67.5–101.8% at intra-day and inter-day intervals, respectively. Relative standard deviations were below 20% for the recovery studies. The extraction method were further validated by performing recovery study and matrix effect test in six different grape varieties from Taiwan and the United States and all resulted in comparable results. Application of the established method to 50 grape samples, resulted in the detection of chlormequat chloride and forchlorfenuron residues in the tested grapes. The results of the method validation and real sample analysis shows the extraction method is therefore suitable for routine monitoring of residue in grapes. Taiwan Food and Drug Administration 2017-09-22 /pmc/articles/PMC9322231/ /pubmed/29567233 http://dx.doi.org/10.1016/j.jfda.2017.08.001 Text en © 2018 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Pu, Chieh-Han
Lin, Shao-Kai
Chuang, Wei-Chen
Shyu, Tsyr-Horng
Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS
title Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS
title_full Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS
title_fullStr Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS
title_full_unstemmed Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS
title_short Modified QuEChERS method for 24 plant growth regulators in grapes using LC-MS/MS
title_sort modified quechers method for 24 plant growth regulators in grapes using lc-ms/ms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322231/
https://www.ncbi.nlm.nih.gov/pubmed/29567233
http://dx.doi.org/10.1016/j.jfda.2017.08.001
work_keys_str_mv AT puchiehhan modifiedquechersmethodfor24plantgrowthregulatorsingrapesusinglcmsms
AT linshaokai modifiedquechersmethodfor24plantgrowthregulatorsingrapesusinglcmsms
AT chuangweichen modifiedquechersmethodfor24plantgrowthregulatorsingrapesusinglcmsms
AT shyutsyrhorng modifiedquechersmethodfor24plantgrowthregulatorsingrapesusinglcmsms