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A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method

Vitamin A is a vital nutritional substances that regulates biological activities including development, but is also associated with disease onset. The extent of vitamin A intake influences the retinoid content in the liver, the most important organ for the storage of vitamin A. Measurement of endoge...

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Autores principales: Yokota, Satoshi, Oshio, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322232/
https://www.ncbi.nlm.nih.gov/pubmed/29567219
http://dx.doi.org/10.1016/j.jfda.2017.07.002
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author Yokota, Satoshi
Oshio, Shigeru
author_facet Yokota, Satoshi
Oshio, Shigeru
author_sort Yokota, Satoshi
collection PubMed
description Vitamin A is a vital nutritional substances that regulates biological activities including development, but is also associated with disease onset. The extent of vitamin A intake influences the retinoid content in the liver, the most important organ for the storage of vitamin A. Measurement of endogenous retinoid in biological samples is important to understand retinoid homeostasis. Here we present a reliable, highly sensitive, and robust method for the quantification of retinol and retinyl palmitate using a reverse-phase HPLC/ UV isocratic method. We determined the impact of chronic dietary vitamin A on retinoid levels in livers of mice fed an AIN-93G semi-purified diet (4 IU/g) compared with an excess vitamin A diet (1000 IU/g) over a period from birth to 10 weeks of age. Coefficients of variation for intra-assays for both retinoids were less than 5%, suggesting a higher reproducibility than any other HPLC/UV gradient method. Limits of detection and quantification for retinol were 0.08 pmol, and 0.27 pmol, respectively, which are remarkably higher than previous results. Supplementation with higher doses of vitamin A over the study period significantly increased liver retinol and retinyl palmitate concentrations in adult mice. The assays described here provide a sensitive and rigorous quantification of endogenous retinol and retinyl palmitate, which can be used to help determine retinoid homeostasis in disease states, such as toxic hepatitis and liver cancer.
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spelling pubmed-93222322022-08-09 A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method Yokota, Satoshi Oshio, Shigeru J Food Drug Anal Original Article Vitamin A is a vital nutritional substances that regulates biological activities including development, but is also associated with disease onset. The extent of vitamin A intake influences the retinoid content in the liver, the most important organ for the storage of vitamin A. Measurement of endogenous retinoid in biological samples is important to understand retinoid homeostasis. Here we present a reliable, highly sensitive, and robust method for the quantification of retinol and retinyl palmitate using a reverse-phase HPLC/ UV isocratic method. We determined the impact of chronic dietary vitamin A on retinoid levels in livers of mice fed an AIN-93G semi-purified diet (4 IU/g) compared with an excess vitamin A diet (1000 IU/g) over a period from birth to 10 weeks of age. Coefficients of variation for intra-assays for both retinoids were less than 5%, suggesting a higher reproducibility than any other HPLC/UV gradient method. Limits of detection and quantification for retinol were 0.08 pmol, and 0.27 pmol, respectively, which are remarkably higher than previous results. Supplementation with higher doses of vitamin A over the study period significantly increased liver retinol and retinyl palmitate concentrations in adult mice. The assays described here provide a sensitive and rigorous quantification of endogenous retinol and retinyl palmitate, which can be used to help determine retinoid homeostasis in disease states, such as toxic hepatitis and liver cancer. Taiwan Food and Drug Administration 2017-07-25 /pmc/articles/PMC9322232/ /pubmed/29567219 http://dx.doi.org/10.1016/j.jfda.2017.07.002 Text en © 2018 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Yokota, Satoshi
Oshio, Shigeru
A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
title A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
title_full A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
title_fullStr A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
title_full_unstemmed A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
title_short A simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
title_sort simple and robust quantitative analysis of retinol and retinyl palmitate using a liquid chromatographic isocratic method
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322232/
https://www.ncbi.nlm.nih.gov/pubmed/29567219
http://dx.doi.org/10.1016/j.jfda.2017.07.002
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