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Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
Numerous sero-epidemiological studies have been initiated to investigate the spread and dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address the concomitant need for serological high-throughput assays, a bead-based multiplex serology assay, specific for SARS-CoV-2, ha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322257/ https://www.ncbi.nlm.nih.gov/pubmed/35889030 http://dx.doi.org/10.3390/microorganisms10071311 |
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author | Jeske, Rima Merle, Uta Müller, Barbara Waterboer, Tim Butt, Julia |
author_facet | Jeske, Rima Merle, Uta Müller, Barbara Waterboer, Tim Butt, Julia |
author_sort | Jeske, Rima |
collection | PubMed |
description | Numerous sero-epidemiological studies have been initiated to investigate the spread and dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address the concomitant need for serological high-throughput assays, a bead-based multiplex serology assay, specific for SARS-CoV-2, had been developed. SARS-CoV-2 serolomics allows for measuring antibody responses to almost the entire SARS-CoV-2 proteome in up to 2000 serum samples per day. To enlarge the pool of eligible sample collection methods, we here test the compatibility of serolomics with dried blood spot (DBS)-derived eluates. Antibody levels of nine SARS-CoV-2 antigens, including the nucleocapsid (N) and receptor-binding domain of the spike protein (S1-RBD), were measured in 142 paired DBS and serum samples. The numeric correlation between the two sample types was high, with a Pearson’s r of 0.88 for both S1-RBD and N and intraclass correlation coefficients of 0.93 and 0.92, respectively. Systematically reduced antibody levels in DBS eluates were compensated by lowering the cutoffs for seropositivity accordingly. This enabled the concordant classification of SARS-CoV-2 seropositivity, without loss in sensitivity. Antibody levels against accessory SARS-CoV-2 antigens also showed a high concordance, demonstrating that DBS-derived eluates are eligible for SARS-CoV-2 serolomics. DBS cards facilitate the collection of blood samples, as they obviate the need for medically trained personnel and can be shipped at room temperature. In combination with SARS-CoV-2 serolomics, DBS cards enable powerful sero-epidemiological studies, thus allowing for the monitoring of patients and epidemiological analyses in resource-poor settings. |
format | Online Article Text |
id | pubmed-9322257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93222572022-07-27 Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics Jeske, Rima Merle, Uta Müller, Barbara Waterboer, Tim Butt, Julia Microorganisms Brief Report Numerous sero-epidemiological studies have been initiated to investigate the spread and dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address the concomitant need for serological high-throughput assays, a bead-based multiplex serology assay, specific for SARS-CoV-2, had been developed. SARS-CoV-2 serolomics allows for measuring antibody responses to almost the entire SARS-CoV-2 proteome in up to 2000 serum samples per day. To enlarge the pool of eligible sample collection methods, we here test the compatibility of serolomics with dried blood spot (DBS)-derived eluates. Antibody levels of nine SARS-CoV-2 antigens, including the nucleocapsid (N) and receptor-binding domain of the spike protein (S1-RBD), were measured in 142 paired DBS and serum samples. The numeric correlation between the two sample types was high, with a Pearson’s r of 0.88 for both S1-RBD and N and intraclass correlation coefficients of 0.93 and 0.92, respectively. Systematically reduced antibody levels in DBS eluates were compensated by lowering the cutoffs for seropositivity accordingly. This enabled the concordant classification of SARS-CoV-2 seropositivity, without loss in sensitivity. Antibody levels against accessory SARS-CoV-2 antigens also showed a high concordance, demonstrating that DBS-derived eluates are eligible for SARS-CoV-2 serolomics. DBS cards facilitate the collection of blood samples, as they obviate the need for medically trained personnel and can be shipped at room temperature. In combination with SARS-CoV-2 serolomics, DBS cards enable powerful sero-epidemiological studies, thus allowing for the monitoring of patients and epidemiological analyses in resource-poor settings. MDPI 2022-06-29 /pmc/articles/PMC9322257/ /pubmed/35889030 http://dx.doi.org/10.3390/microorganisms10071311 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Jeske, Rima Merle, Uta Müller, Barbara Waterboer, Tim Butt, Julia Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics |
title | Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics |
title_full | Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics |
title_fullStr | Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics |
title_full_unstemmed | Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics |
title_short | Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics |
title_sort | performance of dried blood spot samples in sars-cov-2 serolomics |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322257/ https://www.ncbi.nlm.nih.gov/pubmed/35889030 http://dx.doi.org/10.3390/microorganisms10071311 |
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