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Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics

Numerous sero-epidemiological studies have been initiated to investigate the spread and dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address the concomitant need for serological high-throughput assays, a bead-based multiplex serology assay, specific for SARS-CoV-2, ha...

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Autores principales: Jeske, Rima, Merle, Uta, Müller, Barbara, Waterboer, Tim, Butt, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322257/
https://www.ncbi.nlm.nih.gov/pubmed/35889030
http://dx.doi.org/10.3390/microorganisms10071311
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author Jeske, Rima
Merle, Uta
Müller, Barbara
Waterboer, Tim
Butt, Julia
author_facet Jeske, Rima
Merle, Uta
Müller, Barbara
Waterboer, Tim
Butt, Julia
author_sort Jeske, Rima
collection PubMed
description Numerous sero-epidemiological studies have been initiated to investigate the spread and dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address the concomitant need for serological high-throughput assays, a bead-based multiplex serology assay, specific for SARS-CoV-2, had been developed. SARS-CoV-2 serolomics allows for measuring antibody responses to almost the entire SARS-CoV-2 proteome in up to 2000 serum samples per day. To enlarge the pool of eligible sample collection methods, we here test the compatibility of serolomics with dried blood spot (DBS)-derived eluates. Antibody levels of nine SARS-CoV-2 antigens, including the nucleocapsid (N) and receptor-binding domain of the spike protein (S1-RBD), were measured in 142 paired DBS and serum samples. The numeric correlation between the two sample types was high, with a Pearson’s r of 0.88 for both S1-RBD and N and intraclass correlation coefficients of 0.93 and 0.92, respectively. Systematically reduced antibody levels in DBS eluates were compensated by lowering the cutoffs for seropositivity accordingly. This enabled the concordant classification of SARS-CoV-2 seropositivity, without loss in sensitivity. Antibody levels against accessory SARS-CoV-2 antigens also showed a high concordance, demonstrating that DBS-derived eluates are eligible for SARS-CoV-2 serolomics. DBS cards facilitate the collection of blood samples, as they obviate the need for medically trained personnel and can be shipped at room temperature. In combination with SARS-CoV-2 serolomics, DBS cards enable powerful sero-epidemiological studies, thus allowing for the monitoring of patients and epidemiological analyses in resource-poor settings.
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spelling pubmed-93222572022-07-27 Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics Jeske, Rima Merle, Uta Müller, Barbara Waterboer, Tim Butt, Julia Microorganisms Brief Report Numerous sero-epidemiological studies have been initiated to investigate the spread and dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address the concomitant need for serological high-throughput assays, a bead-based multiplex serology assay, specific for SARS-CoV-2, had been developed. SARS-CoV-2 serolomics allows for measuring antibody responses to almost the entire SARS-CoV-2 proteome in up to 2000 serum samples per day. To enlarge the pool of eligible sample collection methods, we here test the compatibility of serolomics with dried blood spot (DBS)-derived eluates. Antibody levels of nine SARS-CoV-2 antigens, including the nucleocapsid (N) and receptor-binding domain of the spike protein (S1-RBD), were measured in 142 paired DBS and serum samples. The numeric correlation between the two sample types was high, with a Pearson’s r of 0.88 for both S1-RBD and N and intraclass correlation coefficients of 0.93 and 0.92, respectively. Systematically reduced antibody levels in DBS eluates were compensated by lowering the cutoffs for seropositivity accordingly. This enabled the concordant classification of SARS-CoV-2 seropositivity, without loss in sensitivity. Antibody levels against accessory SARS-CoV-2 antigens also showed a high concordance, demonstrating that DBS-derived eluates are eligible for SARS-CoV-2 serolomics. DBS cards facilitate the collection of blood samples, as they obviate the need for medically trained personnel and can be shipped at room temperature. In combination with SARS-CoV-2 serolomics, DBS cards enable powerful sero-epidemiological studies, thus allowing for the monitoring of patients and epidemiological analyses in resource-poor settings. MDPI 2022-06-29 /pmc/articles/PMC9322257/ /pubmed/35889030 http://dx.doi.org/10.3390/microorganisms10071311 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Jeske, Rima
Merle, Uta
Müller, Barbara
Waterboer, Tim
Butt, Julia
Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
title Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
title_full Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
title_fullStr Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
title_full_unstemmed Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
title_short Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
title_sort performance of dried blood spot samples in sars-cov-2 serolomics
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322257/
https://www.ncbi.nlm.nih.gov/pubmed/35889030
http://dx.doi.org/10.3390/microorganisms10071311
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