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Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), can be transmitted both horizontally and vertically and there is no available cure or prophylaxis. The control of BKD requires continuous surveillance, which is challenging in aquaculture as well as in programs for conservati...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322471/ https://www.ncbi.nlm.nih.gov/pubmed/35363399 http://dx.doi.org/10.1111/jfd.13614 |
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author | Jansson, Eva Aspán, Anna Comin, Arianna Hjort, Maj Jinnerot, Tomas Axén, Charlotte |
author_facet | Jansson, Eva Aspán, Anna Comin, Arianna Hjort, Maj Jinnerot, Tomas Axén, Charlotte |
author_sort | Jansson, Eva |
collection | PubMed |
description | Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), can be transmitted both horizontally and vertically and there is no available cure or prophylaxis. The control of BKD requires continuous surveillance, which is challenging in aquaculture as well as in programs for conservation and restoration of salmonid fish strains. BKD is a notifiable disease in Sweden and is monitored through the mandatory health control program using a polyclonal ELISA for detection of the Rs p57 protein in kidney. Fish must be killed for sampling, an obvious disadvantage especially regarding valuable broodfish. The present study shows that gill‐/cloacal swabs collected in vivo for real‐time PCR (qPCR(gc)), allow a sensitive and specific detection of Rs. The sensitivity of qPCR(gc) was estimated to 97.8% (credible interval (ci) 93.8%–100%) compared to 98.3% (ci 92.7%–100%) and 48.8% (ci 38.8%–58.8%) of kidney samples for qPCR (qPCR(k)) and ELISA (ELISA(k)) respectively, by use of the Bayesian Latent Class Analysis (BLCA). Since the goal of the program is eradication of BKD the most sensitive test is preferrable. Using qPCR(gc) instead of ELISA(k) will result in a lower false negative rate and can be useful for surveillance in aquaculture and in breeding programs with valuable fish. However, a higher false positive rate warrants confirmatory lethal testing before a previously Rs negative farm is subject to restrictions. |
format | Online Article Text |
id | pubmed-9322471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93224712022-07-30 Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease Jansson, Eva Aspán, Anna Comin, Arianna Hjort, Maj Jinnerot, Tomas Axén, Charlotte J Fish Dis Research Articles Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), can be transmitted both horizontally and vertically and there is no available cure or prophylaxis. The control of BKD requires continuous surveillance, which is challenging in aquaculture as well as in programs for conservation and restoration of salmonid fish strains. BKD is a notifiable disease in Sweden and is monitored through the mandatory health control program using a polyclonal ELISA for detection of the Rs p57 protein in kidney. Fish must be killed for sampling, an obvious disadvantage especially regarding valuable broodfish. The present study shows that gill‐/cloacal swabs collected in vivo for real‐time PCR (qPCR(gc)), allow a sensitive and specific detection of Rs. The sensitivity of qPCR(gc) was estimated to 97.8% (credible interval (ci) 93.8%–100%) compared to 98.3% (ci 92.7%–100%) and 48.8% (ci 38.8%–58.8%) of kidney samples for qPCR (qPCR(k)) and ELISA (ELISA(k)) respectively, by use of the Bayesian Latent Class Analysis (BLCA). Since the goal of the program is eradication of BKD the most sensitive test is preferrable. Using qPCR(gc) instead of ELISA(k) will result in a lower false negative rate and can be useful for surveillance in aquaculture and in breeding programs with valuable fish. However, a higher false positive rate warrants confirmatory lethal testing before a previously Rs negative farm is subject to restrictions. John Wiley and Sons Inc. 2022-04-01 2022-06 /pmc/articles/PMC9322471/ /pubmed/35363399 http://dx.doi.org/10.1111/jfd.13614 Text en © 2022 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Jansson, Eva Aspán, Anna Comin, Arianna Hjort, Maj Jinnerot, Tomas Axén, Charlotte Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease |
title | Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease |
title_full | Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease |
title_fullStr | Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease |
title_full_unstemmed | Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease |
title_short | Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease |
title_sort | non‐lethal sampling for the detection of renibacterium salmoninarum by qpcr for diagnosis of bacterial kidney disease |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322471/ https://www.ncbi.nlm.nih.gov/pubmed/35363399 http://dx.doi.org/10.1111/jfd.13614 |
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