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Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease

Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), can be transmitted both horizontally and vertically and there is no available cure or prophylaxis. The control of BKD requires continuous surveillance, which is challenging in aquaculture as well as in programs for conservati...

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Autores principales: Jansson, Eva, Aspán, Anna, Comin, Arianna, Hjort, Maj, Jinnerot, Tomas, Axén, Charlotte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322471/
https://www.ncbi.nlm.nih.gov/pubmed/35363399
http://dx.doi.org/10.1111/jfd.13614
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author Jansson, Eva
Aspán, Anna
Comin, Arianna
Hjort, Maj
Jinnerot, Tomas
Axén, Charlotte
author_facet Jansson, Eva
Aspán, Anna
Comin, Arianna
Hjort, Maj
Jinnerot, Tomas
Axén, Charlotte
author_sort Jansson, Eva
collection PubMed
description Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), can be transmitted both horizontally and vertically and there is no available cure or prophylaxis. The control of BKD requires continuous surveillance, which is challenging in aquaculture as well as in programs for conservation and restoration of salmonid fish strains. BKD is a notifiable disease in Sweden and is monitored through the mandatory health control program using a polyclonal ELISA for detection of the Rs p57 protein in kidney. Fish must be killed for sampling, an obvious disadvantage especially regarding valuable broodfish. The present study shows that gill‐/cloacal swabs collected in vivo for real‐time PCR (qPCR(gc)), allow a sensitive and specific detection of Rs. The sensitivity of qPCR(gc) was estimated to 97.8% (credible interval (ci) 93.8%–100%) compared to 98.3% (ci 92.7%–100%) and 48.8% (ci 38.8%–58.8%) of kidney samples for qPCR (qPCR(k)) and ELISA (ELISA(k)) respectively, by use of the Bayesian Latent Class Analysis (BLCA). Since the goal of the program is eradication of BKD the most sensitive test is preferrable. Using qPCR(gc) instead of ELISA(k) will result in a lower false negative rate and can be useful for surveillance in aquaculture and in breeding programs with valuable fish. However, a higher false positive rate warrants confirmatory lethal testing before a previously Rs negative farm is subject to restrictions.
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spelling pubmed-93224712022-07-30 Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease Jansson, Eva Aspán, Anna Comin, Arianna Hjort, Maj Jinnerot, Tomas Axén, Charlotte J Fish Dis Research Articles Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), can be transmitted both horizontally and vertically and there is no available cure or prophylaxis. The control of BKD requires continuous surveillance, which is challenging in aquaculture as well as in programs for conservation and restoration of salmonid fish strains. BKD is a notifiable disease in Sweden and is monitored through the mandatory health control program using a polyclonal ELISA for detection of the Rs p57 protein in kidney. Fish must be killed for sampling, an obvious disadvantage especially regarding valuable broodfish. The present study shows that gill‐/cloacal swabs collected in vivo for real‐time PCR (qPCR(gc)), allow a sensitive and specific detection of Rs. The sensitivity of qPCR(gc) was estimated to 97.8% (credible interval (ci) 93.8%–100%) compared to 98.3% (ci 92.7%–100%) and 48.8% (ci 38.8%–58.8%) of kidney samples for qPCR (qPCR(k)) and ELISA (ELISA(k)) respectively, by use of the Bayesian Latent Class Analysis (BLCA). Since the goal of the program is eradication of BKD the most sensitive test is preferrable. Using qPCR(gc) instead of ELISA(k) will result in a lower false negative rate and can be useful for surveillance in aquaculture and in breeding programs with valuable fish. However, a higher false positive rate warrants confirmatory lethal testing before a previously Rs negative farm is subject to restrictions. John Wiley and Sons Inc. 2022-04-01 2022-06 /pmc/articles/PMC9322471/ /pubmed/35363399 http://dx.doi.org/10.1111/jfd.13614 Text en © 2022 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Jansson, Eva
Aspán, Anna
Comin, Arianna
Hjort, Maj
Jinnerot, Tomas
Axén, Charlotte
Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
title Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
title_full Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
title_fullStr Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
title_full_unstemmed Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
title_short Non‐lethal sampling for the detection of Renibacterium salmoninarum by qPCR for diagnosis of bacterial kidney disease
title_sort non‐lethal sampling for the detection of renibacterium salmoninarum by qpcr for diagnosis of bacterial kidney disease
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322471/
https://www.ncbi.nlm.nih.gov/pubmed/35363399
http://dx.doi.org/10.1111/jfd.13614
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