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SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study
The SARS-CoV-2 variant of concern, α, spread worldwide at the beginning of 2021. It was suggested that this variant was associated with a higher risk of mortality than other variants. We aimed to characterize the genetic diversity of SARS-CoV-2 variants isolated from patients with severe COVID-19 an...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322524/ https://www.ncbi.nlm.nih.gov/pubmed/35891509 http://dx.doi.org/10.3390/v14071529 |
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author | Fourati, Slim Audureau, Etienne Arrestier, Romain Marot, Stéphane Dubois, Claire Voiriot, Guillaume Luyt, Charles-Edouard Urbina, Tomas Mayaux, Julien Roque-Afonso, Anne-Marie Pham, Tài Landraud, Luce Visseaux, Benoit Roux, Damien Bellaiche, Raphael L’honneur, Anne-Sophie Ait Hamou, Zakaria Brichler, Ségolène Gaudry, Stéphane Salmona, Maud Clere-Jehl, Raphaël Azoulay, Elie Morand-Joubert, Laurence Marcelin, Anne-Geneviève Chaix, Marie-Laure Descamps, Diane Mekontso Dessap, Armand Rodriguez, Christophe Pawlotsky, Jean-Michel de Prost, Nicolas |
author_facet | Fourati, Slim Audureau, Etienne Arrestier, Romain Marot, Stéphane Dubois, Claire Voiriot, Guillaume Luyt, Charles-Edouard Urbina, Tomas Mayaux, Julien Roque-Afonso, Anne-Marie Pham, Tài Landraud, Luce Visseaux, Benoit Roux, Damien Bellaiche, Raphael L’honneur, Anne-Sophie Ait Hamou, Zakaria Brichler, Ségolène Gaudry, Stéphane Salmona, Maud Clere-Jehl, Raphaël Azoulay, Elie Morand-Joubert, Laurence Marcelin, Anne-Geneviève Chaix, Marie-Laure Descamps, Diane Mekontso Dessap, Armand Rodriguez, Christophe Pawlotsky, Jean-Michel de Prost, Nicolas |
author_sort | Fourati, Slim |
collection | PubMed |
description | The SARS-CoV-2 variant of concern, α, spread worldwide at the beginning of 2021. It was suggested that this variant was associated with a higher risk of mortality than other variants. We aimed to characterize the genetic diversity of SARS-CoV-2 variants isolated from patients with severe COVID-19 and unravel the relationships between specific viral mutations/mutational patterns and clinical outcomes. This is a prospective multicenter observational cohort study. Patients aged ≥18 years admitted to 11 intensive care units (ICUs) in hospitals in the Greater Paris area for SARS-CoV-2 infection and acute respiratory failure between 1 October 2020 and 30 May 2021 were included. The primary clinical endpoint was day-28 mortality. Full-length SARS-CoV-2 genomes were sequenced by means of next-generation sequencing (Illumina COVIDSeq). In total, 413 patients were included, 183 (44.3%) were infected with pre-existing variants, 197 (47.7%) were infected with variant α, and 33 (8.0%) were infected with other variants. The patients infected with pre-existing variants were significantly older (64.9 ± 11.9 vs. 60.5 ± 11.8 years; p = 0.0005) and had more frequent COPD (11.5% vs. 4.1%; p = 0.009) and higher SOFA scores (4 [3–8] vs. 3 [2–4]; 0.0002). The day-28 mortality was no different between the patients infected with pre-existing, α, or other variants (31.1% vs. 26.2% vs. 30.3%; p = 0.550). There was no association between day-28 mortality and specific variants or the presence of specific mutations. At ICU admission, the patients infected with pre-existing variants had a different clinical presentation from those infected with variant α, but mortality did not differ between these groups. There was no association between specific variants or SARS-CoV-2 genome mutational pattern and day-28 mortality. |
format | Online Article Text |
id | pubmed-9322524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93225242022-07-27 SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study Fourati, Slim Audureau, Etienne Arrestier, Romain Marot, Stéphane Dubois, Claire Voiriot, Guillaume Luyt, Charles-Edouard Urbina, Tomas Mayaux, Julien Roque-Afonso, Anne-Marie Pham, Tài Landraud, Luce Visseaux, Benoit Roux, Damien Bellaiche, Raphael L’honneur, Anne-Sophie Ait Hamou, Zakaria Brichler, Ségolène Gaudry, Stéphane Salmona, Maud Clere-Jehl, Raphaël Azoulay, Elie Morand-Joubert, Laurence Marcelin, Anne-Geneviève Chaix, Marie-Laure Descamps, Diane Mekontso Dessap, Armand Rodriguez, Christophe Pawlotsky, Jean-Michel de Prost, Nicolas Viruses Article The SARS-CoV-2 variant of concern, α, spread worldwide at the beginning of 2021. It was suggested that this variant was associated with a higher risk of mortality than other variants. We aimed to characterize the genetic diversity of SARS-CoV-2 variants isolated from patients with severe COVID-19 and unravel the relationships between specific viral mutations/mutational patterns and clinical outcomes. This is a prospective multicenter observational cohort study. Patients aged ≥18 years admitted to 11 intensive care units (ICUs) in hospitals in the Greater Paris area for SARS-CoV-2 infection and acute respiratory failure between 1 October 2020 and 30 May 2021 were included. The primary clinical endpoint was day-28 mortality. Full-length SARS-CoV-2 genomes were sequenced by means of next-generation sequencing (Illumina COVIDSeq). In total, 413 patients were included, 183 (44.3%) were infected with pre-existing variants, 197 (47.7%) were infected with variant α, and 33 (8.0%) were infected with other variants. The patients infected with pre-existing variants were significantly older (64.9 ± 11.9 vs. 60.5 ± 11.8 years; p = 0.0005) and had more frequent COPD (11.5% vs. 4.1%; p = 0.009) and higher SOFA scores (4 [3–8] vs. 3 [2–4]; 0.0002). The day-28 mortality was no different between the patients infected with pre-existing, α, or other variants (31.1% vs. 26.2% vs. 30.3%; p = 0.550). There was no association between day-28 mortality and specific variants or the presence of specific mutations. At ICU admission, the patients infected with pre-existing variants had a different clinical presentation from those infected with variant α, but mortality did not differ between these groups. There was no association between specific variants or SARS-CoV-2 genome mutational pattern and day-28 mortality. MDPI 2022-07-13 /pmc/articles/PMC9322524/ /pubmed/35891509 http://dx.doi.org/10.3390/v14071529 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fourati, Slim Audureau, Etienne Arrestier, Romain Marot, Stéphane Dubois, Claire Voiriot, Guillaume Luyt, Charles-Edouard Urbina, Tomas Mayaux, Julien Roque-Afonso, Anne-Marie Pham, Tài Landraud, Luce Visseaux, Benoit Roux, Damien Bellaiche, Raphael L’honneur, Anne-Sophie Ait Hamou, Zakaria Brichler, Ségolène Gaudry, Stéphane Salmona, Maud Clere-Jehl, Raphaël Azoulay, Elie Morand-Joubert, Laurence Marcelin, Anne-Geneviève Chaix, Marie-Laure Descamps, Diane Mekontso Dessap, Armand Rodriguez, Christophe Pawlotsky, Jean-Michel de Prost, Nicolas SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study |
title | SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study |
title_full | SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study |
title_fullStr | SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study |
title_full_unstemmed | SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study |
title_short | SARS-CoV-2 Genomic Characteristics and Clinical Impact of SARS-CoV-2 Viral Diversity in Critically Ill COVID-19 Patients: A Prospective Multicenter Cohort Study |
title_sort | sars-cov-2 genomic characteristics and clinical impact of sars-cov-2 viral diversity in critically ill covid-19 patients: a prospective multicenter cohort study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322524/ https://www.ncbi.nlm.nih.gov/pubmed/35891509 http://dx.doi.org/10.3390/v14071529 |
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