Cargando…

Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation

The bioactive and biocompatible properties of hydroxyapatite (HAp) promote the osseointegration process. HAp is widely used in biomedical applications, especially in orthopedics, as well as a coating material for metallic implants. We obtained composite coatings based on HAp, chitosan (CS), and FGF2...

Descripción completa

Detalles Bibliográficos
Autores principales: Grumezescu, Valentina, Grumezescu, Alexandru Mihai, Ficai, Anton, Negut, Irina, Vasile, Bogdan Ștefan, Gălățeanu, Bianca, Hudiță, Ariana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322700/
https://www.ncbi.nlm.nih.gov/pubmed/35890714
http://dx.doi.org/10.3390/polym14142934
_version_ 1784756369501454336
author Grumezescu, Valentina
Grumezescu, Alexandru Mihai
Ficai, Anton
Negut, Irina
Vasile, Bogdan Ștefan
Gălățeanu, Bianca
Hudiță, Ariana
author_facet Grumezescu, Valentina
Grumezescu, Alexandru Mihai
Ficai, Anton
Negut, Irina
Vasile, Bogdan Ștefan
Gălățeanu, Bianca
Hudiță, Ariana
author_sort Grumezescu, Valentina
collection PubMed
description The bioactive and biocompatible properties of hydroxyapatite (HAp) promote the osseointegration process. HAp is widely used in biomedical applications, especially in orthopedics, as well as a coating material for metallic implants. We obtained composite coatings based on HAp, chitosan (CS), and FGF2 by a matrix-assisted pulsed laser evaporation (MAPLE) technique. The coatings were physico-chemically investigated by means of X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Infrared Microscopy (IRM), and Scanning Electron Microscopy (SEM). Further, biological investigations were performed. The MAPLE-composite coatings were tested in vitro on the MC3T3-E1 cell line in order to endorse cell attachment and growth without toxic effects and to promote pre-osteoblast differentiation towards the osteogenic lineage. These coatings can be considered suitable for bone tissue engineering applications that lack toxicity and promotes cell adhesion and proliferation while also sustaining the differentiation of pre-osteoblasts towards mature bone cells.
format Online
Article
Text
id pubmed-9322700
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93227002022-07-27 Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation Grumezescu, Valentina Grumezescu, Alexandru Mihai Ficai, Anton Negut, Irina Vasile, Bogdan Ștefan Gălățeanu, Bianca Hudiță, Ariana Polymers (Basel) Article The bioactive and biocompatible properties of hydroxyapatite (HAp) promote the osseointegration process. HAp is widely used in biomedical applications, especially in orthopedics, as well as a coating material for metallic implants. We obtained composite coatings based on HAp, chitosan (CS), and FGF2 by a matrix-assisted pulsed laser evaporation (MAPLE) technique. The coatings were physico-chemically investigated by means of X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Infrared Microscopy (IRM), and Scanning Electron Microscopy (SEM). Further, biological investigations were performed. The MAPLE-composite coatings were tested in vitro on the MC3T3-E1 cell line in order to endorse cell attachment and growth without toxic effects and to promote pre-osteoblast differentiation towards the osteogenic lineage. These coatings can be considered suitable for bone tissue engineering applications that lack toxicity and promotes cell adhesion and proliferation while also sustaining the differentiation of pre-osteoblasts towards mature bone cells. MDPI 2022-07-20 /pmc/articles/PMC9322700/ /pubmed/35890714 http://dx.doi.org/10.3390/polym14142934 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grumezescu, Valentina
Grumezescu, Alexandru Mihai
Ficai, Anton
Negut, Irina
Vasile, Bogdan Ștefan
Gălățeanu, Bianca
Hudiță, Ariana
Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
title Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
title_full Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
title_fullStr Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
title_full_unstemmed Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
title_short Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
title_sort composite coatings for osteoblast growth attachment fabricated by matrix-assisted pulsed laser evaporation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322700/
https://www.ncbi.nlm.nih.gov/pubmed/35890714
http://dx.doi.org/10.3390/polym14142934
work_keys_str_mv AT grumezescuvalentina compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation
AT grumezescualexandrumihai compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation
AT ficaianton compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation
AT negutirina compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation
AT vasilebogdanstefan compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation
AT galateanubianca compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation
AT huditaariana compositecoatingsforosteoblastgrowthattachmentfabricatedbymatrixassistedpulsedlaserevaporation