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Long-Term Mastication Changed Salivary Metabolomic Profiles
Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322701/ https://www.ncbi.nlm.nih.gov/pubmed/35888784 http://dx.doi.org/10.3390/metabo12070660 |
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author | Saeki, Yoji Takenouchi, Akane Otani, Etsuyo Kim, Minji Aizawa, Yumi Aita, Yasuko Tomita, Atsumi Sugimoto, Masahiro Matsukubo, Takashi |
author_facet | Saeki, Yoji Takenouchi, Akane Otani, Etsuyo Kim, Minji Aizawa, Yumi Aita, Yasuko Tomita, Atsumi Sugimoto, Masahiro Matsukubo, Takashi |
author_sort | Saeki, Yoji |
collection | PubMed |
description | Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here, we evaluated the effect of long-term mastication on salivary metabolomic profiles. Young women with good oral hygiene (20.8 ± 0.3 years, n = 17) participated. They were prohibited from chewing gum during control periods (4 weeks each) and were instructed to chew a piece of gum base seven times a day for 10 min each time during the intervention period. Paired samples of unstimulated whole saliva collected on the last day of the control and intervention period were compared. Liquid chromatography–time-of-flight mass spectrometry successfully quantified 85 metabolites, of which 41 showed significant differences (p < 0.05, Wilcoxon paired test corrected by false discovery rate). Except for a few metabolites, such as citrate, most metabolites showed lower concentrations after the intervention. The pathways related to glycogenic amino acids, such as alanine, arginine, and glutamine, altered considerably. This study suggests that long-term mastication induces unstimulated salivary component-level changes. |
format | Online Article Text |
id | pubmed-9322701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93227012022-07-27 Long-Term Mastication Changed Salivary Metabolomic Profiles Saeki, Yoji Takenouchi, Akane Otani, Etsuyo Kim, Minji Aizawa, Yumi Aita, Yasuko Tomita, Atsumi Sugimoto, Masahiro Matsukubo, Takashi Metabolites Article Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here, we evaluated the effect of long-term mastication on salivary metabolomic profiles. Young women with good oral hygiene (20.8 ± 0.3 years, n = 17) participated. They were prohibited from chewing gum during control periods (4 weeks each) and were instructed to chew a piece of gum base seven times a day for 10 min each time during the intervention period. Paired samples of unstimulated whole saliva collected on the last day of the control and intervention period were compared. Liquid chromatography–time-of-flight mass spectrometry successfully quantified 85 metabolites, of which 41 showed significant differences (p < 0.05, Wilcoxon paired test corrected by false discovery rate). Except for a few metabolites, such as citrate, most metabolites showed lower concentrations after the intervention. The pathways related to glycogenic amino acids, such as alanine, arginine, and glutamine, altered considerably. This study suggests that long-term mastication induces unstimulated salivary component-level changes. MDPI 2022-07-18 /pmc/articles/PMC9322701/ /pubmed/35888784 http://dx.doi.org/10.3390/metabo12070660 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Saeki, Yoji Takenouchi, Akane Otani, Etsuyo Kim, Minji Aizawa, Yumi Aita, Yasuko Tomita, Atsumi Sugimoto, Masahiro Matsukubo, Takashi Long-Term Mastication Changed Salivary Metabolomic Profiles |
title | Long-Term Mastication Changed Salivary Metabolomic Profiles |
title_full | Long-Term Mastication Changed Salivary Metabolomic Profiles |
title_fullStr | Long-Term Mastication Changed Salivary Metabolomic Profiles |
title_full_unstemmed | Long-Term Mastication Changed Salivary Metabolomic Profiles |
title_short | Long-Term Mastication Changed Salivary Metabolomic Profiles |
title_sort | long-term mastication changed salivary metabolomic profiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322701/ https://www.ncbi.nlm.nih.gov/pubmed/35888784 http://dx.doi.org/10.3390/metabo12070660 |
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