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Long-Term Mastication Changed Salivary Metabolomic Profiles

Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here,...

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Autores principales: Saeki, Yoji, Takenouchi, Akane, Otani, Etsuyo, Kim, Minji, Aizawa, Yumi, Aita, Yasuko, Tomita, Atsumi, Sugimoto, Masahiro, Matsukubo, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322701/
https://www.ncbi.nlm.nih.gov/pubmed/35888784
http://dx.doi.org/10.3390/metabo12070660
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author Saeki, Yoji
Takenouchi, Akane
Otani, Etsuyo
Kim, Minji
Aizawa, Yumi
Aita, Yasuko
Tomita, Atsumi
Sugimoto, Masahiro
Matsukubo, Takashi
author_facet Saeki, Yoji
Takenouchi, Akane
Otani, Etsuyo
Kim, Minji
Aizawa, Yumi
Aita, Yasuko
Tomita, Atsumi
Sugimoto, Masahiro
Matsukubo, Takashi
author_sort Saeki, Yoji
collection PubMed
description Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here, we evaluated the effect of long-term mastication on salivary metabolomic profiles. Young women with good oral hygiene (20.8 ± 0.3 years, n = 17) participated. They were prohibited from chewing gum during control periods (4 weeks each) and were instructed to chew a piece of gum base seven times a day for 10 min each time during the intervention period. Paired samples of unstimulated whole saliva collected on the last day of the control and intervention period were compared. Liquid chromatography–time-of-flight mass spectrometry successfully quantified 85 metabolites, of which 41 showed significant differences (p < 0.05, Wilcoxon paired test corrected by false discovery rate). Except for a few metabolites, such as citrate, most metabolites showed lower concentrations after the intervention. The pathways related to glycogenic amino acids, such as alanine, arginine, and glutamine, altered considerably. This study suggests that long-term mastication induces unstimulated salivary component-level changes.
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spelling pubmed-93227012022-07-27 Long-Term Mastication Changed Salivary Metabolomic Profiles Saeki, Yoji Takenouchi, Akane Otani, Etsuyo Kim, Minji Aizawa, Yumi Aita, Yasuko Tomita, Atsumi Sugimoto, Masahiro Matsukubo, Takashi Metabolites Article Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here, we evaluated the effect of long-term mastication on salivary metabolomic profiles. Young women with good oral hygiene (20.8 ± 0.3 years, n = 17) participated. They were prohibited from chewing gum during control periods (4 weeks each) and were instructed to chew a piece of gum base seven times a day for 10 min each time during the intervention period. Paired samples of unstimulated whole saliva collected on the last day of the control and intervention period were compared. Liquid chromatography–time-of-flight mass spectrometry successfully quantified 85 metabolites, of which 41 showed significant differences (p < 0.05, Wilcoxon paired test corrected by false discovery rate). Except for a few metabolites, such as citrate, most metabolites showed lower concentrations after the intervention. The pathways related to glycogenic amino acids, such as alanine, arginine, and glutamine, altered considerably. This study suggests that long-term mastication induces unstimulated salivary component-level changes. MDPI 2022-07-18 /pmc/articles/PMC9322701/ /pubmed/35888784 http://dx.doi.org/10.3390/metabo12070660 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saeki, Yoji
Takenouchi, Akane
Otani, Etsuyo
Kim, Minji
Aizawa, Yumi
Aita, Yasuko
Tomita, Atsumi
Sugimoto, Masahiro
Matsukubo, Takashi
Long-Term Mastication Changed Salivary Metabolomic Profiles
title Long-Term Mastication Changed Salivary Metabolomic Profiles
title_full Long-Term Mastication Changed Salivary Metabolomic Profiles
title_fullStr Long-Term Mastication Changed Salivary Metabolomic Profiles
title_full_unstemmed Long-Term Mastication Changed Salivary Metabolomic Profiles
title_short Long-Term Mastication Changed Salivary Metabolomic Profiles
title_sort long-term mastication changed salivary metabolomic profiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322701/
https://www.ncbi.nlm.nih.gov/pubmed/35888784
http://dx.doi.org/10.3390/metabo12070660
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