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Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?

Aflatoxin B1 (AFB1) is a major food safety concern, threatening the health of humans and animals. Bentonite (BEN) is an aluminosilicate clay used as a feed additive to reduce AFB1 presence in contaminated feedstuff. So far, few studies have characterized BEN toxicity and efficacy in vitro. In this s...

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Autores principales: Mucignat, Greta, Bassan, Irene, Giantin, Mery, Pauletto, Marianna, Bardhi, Anisa, Iori, Silvia, Lopparelli, Rosa Maria, Barbarossa, Andrea, Zaghini, Anna, Novelli, Enrico, Dacasto, Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322703/
https://www.ncbi.nlm.nih.gov/pubmed/35878173
http://dx.doi.org/10.3390/toxins14070435
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author Mucignat, Greta
Bassan, Irene
Giantin, Mery
Pauletto, Marianna
Bardhi, Anisa
Iori, Silvia
Lopparelli, Rosa Maria
Barbarossa, Andrea
Zaghini, Anna
Novelli, Enrico
Dacasto, Mauro
author_facet Mucignat, Greta
Bassan, Irene
Giantin, Mery
Pauletto, Marianna
Bardhi, Anisa
Iori, Silvia
Lopparelli, Rosa Maria
Barbarossa, Andrea
Zaghini, Anna
Novelli, Enrico
Dacasto, Mauro
author_sort Mucignat, Greta
collection PubMed
description Aflatoxin B1 (AFB1) is a major food safety concern, threatening the health of humans and animals. Bentonite (BEN) is an aluminosilicate clay used as a feed additive to reduce AFB1 presence in contaminated feedstuff. So far, few studies have characterized BEN toxicity and efficacy in vitro. In this study, cytotoxicity (WST-1 test), the effects on cell permeability (trans-epithelial electrical resistance and lucifer yellow dye incorporation), and transcriptional changes (RNA-seq) caused by BEN, AFB1 and their combination (AFB1 + BEN) were investigated in Caco-2 cells. Up to 0.1 mg/mL, BEN did not affect cell viability and permeability, but it reduced AFB1 cytotoxicity; however, at higher concentrations, BEN was cytotoxic. As to RNA-seq, 0.1 mg/mL BEN did not show effects on cell transcriptome, confirming that the interaction between BEN and AFB1 occurs in the medium. Data from AFB1 and AFB1 + BEN suggested AFB1 provoked most of the transcriptional changes, whereas BEN was preventive. The most interesting AFB1-targeted pathways for which BEN was effective were cell integrity, xenobiotic metabolism and transporters, basal metabolism, inflammation and immune response, p53 biological network, apoptosis and carcinogenesis. To our knowledge, this is the first study assessing the in vitro toxicity and whole-transcriptomic effects of BEN, alone or in the presence of AFB1.
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spelling pubmed-93227032022-07-27 Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure? Mucignat, Greta Bassan, Irene Giantin, Mery Pauletto, Marianna Bardhi, Anisa Iori, Silvia Lopparelli, Rosa Maria Barbarossa, Andrea Zaghini, Anna Novelli, Enrico Dacasto, Mauro Toxins (Basel) Article Aflatoxin B1 (AFB1) is a major food safety concern, threatening the health of humans and animals. Bentonite (BEN) is an aluminosilicate clay used as a feed additive to reduce AFB1 presence in contaminated feedstuff. So far, few studies have characterized BEN toxicity and efficacy in vitro. In this study, cytotoxicity (WST-1 test), the effects on cell permeability (trans-epithelial electrical resistance and lucifer yellow dye incorporation), and transcriptional changes (RNA-seq) caused by BEN, AFB1 and their combination (AFB1 + BEN) were investigated in Caco-2 cells. Up to 0.1 mg/mL, BEN did not affect cell viability and permeability, but it reduced AFB1 cytotoxicity; however, at higher concentrations, BEN was cytotoxic. As to RNA-seq, 0.1 mg/mL BEN did not show effects on cell transcriptome, confirming that the interaction between BEN and AFB1 occurs in the medium. Data from AFB1 and AFB1 + BEN suggested AFB1 provoked most of the transcriptional changes, whereas BEN was preventive. The most interesting AFB1-targeted pathways for which BEN was effective were cell integrity, xenobiotic metabolism and transporters, basal metabolism, inflammation and immune response, p53 biological network, apoptosis and carcinogenesis. To our knowledge, this is the first study assessing the in vitro toxicity and whole-transcriptomic effects of BEN, alone or in the presence of AFB1. MDPI 2022-06-26 /pmc/articles/PMC9322703/ /pubmed/35878173 http://dx.doi.org/10.3390/toxins14070435 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mucignat, Greta
Bassan, Irene
Giantin, Mery
Pauletto, Marianna
Bardhi, Anisa
Iori, Silvia
Lopparelli, Rosa Maria
Barbarossa, Andrea
Zaghini, Anna
Novelli, Enrico
Dacasto, Mauro
Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?
title Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?
title_full Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?
title_fullStr Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?
title_full_unstemmed Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?
title_short Does Bentonite Cause Cytotoxic and Whole-Transcriptomic Adverse Effects in Enterocytes When Used to Reduce Aflatoxin B1 Exposure?
title_sort does bentonite cause cytotoxic and whole-transcriptomic adverse effects in enterocytes when used to reduce aflatoxin b1 exposure?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322703/
https://www.ncbi.nlm.nih.gov/pubmed/35878173
http://dx.doi.org/10.3390/toxins14070435
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