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Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice

Apparent amylose content (AAC) is one of the most important parameters in rice quality evaluation. In this study, four rice reference materials used to test rice AAC were developed. The AAC of rice reference materials were measured by a spectrophotometric method with a defatting procedure, calibrate...

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Autores principales: Shao, Yafang, Zhu, Dawei, Yu, Jing, Fang, Changyun, Mou, Renxiang, Hu, Xianqiao, Zhu, Zhiwei, Chen, Mingxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322866/
https://www.ncbi.nlm.nih.gov/pubmed/35889518
http://dx.doi.org/10.3390/molecules27144647
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author Shao, Yafang
Zhu, Dawei
Yu, Jing
Fang, Changyun
Mou, Renxiang
Hu, Xianqiao
Zhu, Zhiwei
Chen, Mingxue
author_facet Shao, Yafang
Zhu, Dawei
Yu, Jing
Fang, Changyun
Mou, Renxiang
Hu, Xianqiao
Zhu, Zhiwei
Chen, Mingxue
author_sort Shao, Yafang
collection PubMed
description Apparent amylose content (AAC) is one of the most important parameters in rice quality evaluation. In this study, four rice reference materials used to test rice AAC were developed. The AAC of rice reference materials were measured by a spectrophotometric method with a defatting procedure, calibrated from potato amylose and waxy rice amylopectin at the absorption wavelengths of 620 and 720 nm. Homogeneity test (n = 20) was judged by F-test based on the mean squares of among and within bottles, and short- and long-term stability monitoring was performed by T-test to check if there was significant degradation at the delivery temperature of under 40 °C (14 days) and at 0–4 °C storage condition (18 months), respectively. After joint evaluation by ten laboratories, Dixion and Cochran statistical analyses were presented. The expanded uncertainties were calculated based on the uncertainty of homogeneity, short- and long-term stability, and inter-laboratory validation containing factor k = 2. It found that the four reference materials were homogenous and stable, and had the AAC (g/100 g, k = 2) of 2.96 ± 1.01, 10.68 ± 0.66, 17.18 ± 1.04, and 16.09 ± 1.29, respectively, at 620 nm, and 1.46 ± 0.49, 10.44 ± 0.56, 16.82 ± 0.75, and 24.33 ± 0.52, respectively, at 720 nm. It was indicated that 720 nm was more suitable for the determination of rice AAC with lower uncertainties. The determinations of the AAC of 11 rice varieties were carried out by two methods, the method without defatting and with calibration from the four rice reference materials and the method with a defatting procedure and calibrating from potato amylose and waxy rice amylopectin. It confirmed that the undefatted rice reference materials could achieve satisfactory results to test the rice samples with the AAC ranging from 1 to 25 g/100 g. It would greatly reduce the time cost and improve testing efficiency and applicability, and provide technical support for the high-quality development of the rice industry.
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spelling pubmed-93228662022-07-27 Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice Shao, Yafang Zhu, Dawei Yu, Jing Fang, Changyun Mou, Renxiang Hu, Xianqiao Zhu, Zhiwei Chen, Mingxue Molecules Article Apparent amylose content (AAC) is one of the most important parameters in rice quality evaluation. In this study, four rice reference materials used to test rice AAC were developed. The AAC of rice reference materials were measured by a spectrophotometric method with a defatting procedure, calibrated from potato amylose and waxy rice amylopectin at the absorption wavelengths of 620 and 720 nm. Homogeneity test (n = 20) was judged by F-test based on the mean squares of among and within bottles, and short- and long-term stability monitoring was performed by T-test to check if there was significant degradation at the delivery temperature of under 40 °C (14 days) and at 0–4 °C storage condition (18 months), respectively. After joint evaluation by ten laboratories, Dixion and Cochran statistical analyses were presented. The expanded uncertainties were calculated based on the uncertainty of homogeneity, short- and long-term stability, and inter-laboratory validation containing factor k = 2. It found that the four reference materials were homogenous and stable, and had the AAC (g/100 g, k = 2) of 2.96 ± 1.01, 10.68 ± 0.66, 17.18 ± 1.04, and 16.09 ± 1.29, respectively, at 620 nm, and 1.46 ± 0.49, 10.44 ± 0.56, 16.82 ± 0.75, and 24.33 ± 0.52, respectively, at 720 nm. It was indicated that 720 nm was more suitable for the determination of rice AAC with lower uncertainties. The determinations of the AAC of 11 rice varieties were carried out by two methods, the method without defatting and with calibration from the four rice reference materials and the method with a defatting procedure and calibrating from potato amylose and waxy rice amylopectin. It confirmed that the undefatted rice reference materials could achieve satisfactory results to test the rice samples with the AAC ranging from 1 to 25 g/100 g. It would greatly reduce the time cost and improve testing efficiency and applicability, and provide technical support for the high-quality development of the rice industry. MDPI 2022-07-21 /pmc/articles/PMC9322866/ /pubmed/35889518 http://dx.doi.org/10.3390/molecules27144647 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shao, Yafang
Zhu, Dawei
Yu, Jing
Fang, Changyun
Mou, Renxiang
Hu, Xianqiao
Zhu, Zhiwei
Chen, Mingxue
Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice
title Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice
title_full Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice
title_fullStr Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice
title_full_unstemmed Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice
title_short Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice
title_sort development of certified reference materials for the determination of apparent amylose content in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322866/
https://www.ncbi.nlm.nih.gov/pubmed/35889518
http://dx.doi.org/10.3390/molecules27144647
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