Cargando…

Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein

Transcription factors (TFs) play an important role in plant development; however, their role during viral infection largely remains unknown. The present study was designed to uncover the role transcription factors play in Cucumber mosaic virus (CMV) infection. During the screening of an Arabidopsis...

Descripción completa

Detalles Bibliográficos
Autores principales: Rattan, Usha Kumari, Kumar, Surender, Kumari, Reenu, Bharti, Monika, Hallan, Vipin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323240/
https://www.ncbi.nlm.nih.gov/pubmed/35890032
http://dx.doi.org/10.3390/pathogens11070788
_version_ 1784756501647196160
author Rattan, Usha Kumari
Kumar, Surender
Kumari, Reenu
Bharti, Monika
Hallan, Vipin
author_facet Rattan, Usha Kumari
Kumar, Surender
Kumari, Reenu
Bharti, Monika
Hallan, Vipin
author_sort Rattan, Usha Kumari
collection PubMed
description Transcription factors (TFs) play an important role in plant development; however, their role during viral infection largely remains unknown. The present study was designed to uncover the role transcription factors play in Cucumber mosaic virus (CMV) infection. During the screening of an Arabidopsis thaliana (Col-0) transcription factor library, using the CMV 2b protein as bait in the yeast two-hybrid system, the 2b protein interacted with Homeobox protein 27 (HB27). HB27 belongs to the zinc finger homeodomain family and is known to have a regulatory role in flower development, and responses to biotic and abiotic stress. The interaction between CMV 2b and HB27 proteins was further validated using in planta (bimolecular fluorescence complementation assay) and in vitro far-Western blotting (FWB) methods. In the bimolecular fluorescence complementation assay, these proteins reconstituted YFP fluorescence in the nucleus and the cytoplasmic region as small fluorescent dots. In FWB, positive interaction was detected using bait anti-MYC antibody on the target HB27-HA protein. During CMV infection, upregulation (~3-fold) of the HB27 transcript was observed at 14 days post-infection (dpi) in A. thaliana plants, and expression declined to the same as healthy plants at 21 dpi. To understand the role of the HB27 protein during CMV infection, virus accumulation was determined in HB27-overexpressing (HB27 OE) and knockout mutants. In HB27-overexpressing lines, infected plants developed mild symptoms, accumulating a lower virus titer at 21 dpi compared to wild-type plants. Additionally, knockout HB27 mutants had more severe symptoms and a higher viral accumulation than wild-type plants. These results indicate that HB27 plays an important role in the regulation of plant defense against plant virus infection.
format Online
Article
Text
id pubmed-9323240
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93232402022-07-27 Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein Rattan, Usha Kumari Kumar, Surender Kumari, Reenu Bharti, Monika Hallan, Vipin Pathogens Article Transcription factors (TFs) play an important role in plant development; however, their role during viral infection largely remains unknown. The present study was designed to uncover the role transcription factors play in Cucumber mosaic virus (CMV) infection. During the screening of an Arabidopsis thaliana (Col-0) transcription factor library, using the CMV 2b protein as bait in the yeast two-hybrid system, the 2b protein interacted with Homeobox protein 27 (HB27). HB27 belongs to the zinc finger homeodomain family and is known to have a regulatory role in flower development, and responses to biotic and abiotic stress. The interaction between CMV 2b and HB27 proteins was further validated using in planta (bimolecular fluorescence complementation assay) and in vitro far-Western blotting (FWB) methods. In the bimolecular fluorescence complementation assay, these proteins reconstituted YFP fluorescence in the nucleus and the cytoplasmic region as small fluorescent dots. In FWB, positive interaction was detected using bait anti-MYC antibody on the target HB27-HA protein. During CMV infection, upregulation (~3-fold) of the HB27 transcript was observed at 14 days post-infection (dpi) in A. thaliana plants, and expression declined to the same as healthy plants at 21 dpi. To understand the role of the HB27 protein during CMV infection, virus accumulation was determined in HB27-overexpressing (HB27 OE) and knockout mutants. In HB27-overexpressing lines, infected plants developed mild symptoms, accumulating a lower virus titer at 21 dpi compared to wild-type plants. Additionally, knockout HB27 mutants had more severe symptoms and a higher viral accumulation than wild-type plants. These results indicate that HB27 plays an important role in the regulation of plant defense against plant virus infection. MDPI 2022-07-12 /pmc/articles/PMC9323240/ /pubmed/35890032 http://dx.doi.org/10.3390/pathogens11070788 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rattan, Usha Kumari
Kumar, Surender
Kumari, Reenu
Bharti, Monika
Hallan, Vipin
Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein
title Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein
title_full Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein
title_fullStr Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein
title_full_unstemmed Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein
title_short Homeobox 27, a Homeodomain Transcription Factor, Confers Tolerances to CMV by Associating with Cucumber Mosaic Virus 2b Protein
title_sort homeobox 27, a homeodomain transcription factor, confers tolerances to cmv by associating with cucumber mosaic virus 2b protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323240/
https://www.ncbi.nlm.nih.gov/pubmed/35890032
http://dx.doi.org/10.3390/pathogens11070788
work_keys_str_mv AT rattanushakumari homeobox27ahomeodomaintranscriptionfactorconferstolerancestocmvbyassociatingwithcucumbermosaicvirus2bprotein
AT kumarsurender homeobox27ahomeodomaintranscriptionfactorconferstolerancestocmvbyassociatingwithcucumbermosaicvirus2bprotein
AT kumarireenu homeobox27ahomeodomaintranscriptionfactorconferstolerancestocmvbyassociatingwithcucumbermosaicvirus2bprotein
AT bhartimonika homeobox27ahomeodomaintranscriptionfactorconferstolerancestocmvbyassociatingwithcucumbermosaicvirus2bprotein
AT hallanvipin homeobox27ahomeodomaintranscriptionfactorconferstolerancestocmvbyassociatingwithcucumbermosaicvirus2bprotein