Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe

MiRNA-targeted therapy is an active research field in precision cancer therapy. Studying the effect of miRNA expression changes on apoptosis is important for evaluating miRNA-targeted therapy and realizing personalized precision therapy for cancer patients. Here, a new fluorescent nanoprobe was desi...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Mingyao, Chen, Zhe, Ge, Chuandong, Hu, Wei, Xu, Jing, Yang, Limin, Luan, Mingming, Wang, Nianxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323288/
https://www.ncbi.nlm.nih.gov/pubmed/35890245
http://dx.doi.org/10.3390/pharmaceutics14071349
_version_ 1784756513568456704
author Ren, Mingyao
Chen, Zhe
Ge, Chuandong
Hu, Wei
Xu, Jing
Yang, Limin
Luan, Mingming
Wang, Nianxing
author_facet Ren, Mingyao
Chen, Zhe
Ge, Chuandong
Hu, Wei
Xu, Jing
Yang, Limin
Luan, Mingming
Wang, Nianxing
author_sort Ren, Mingyao
collection PubMed
description MiRNA-targeted therapy is an active research field in precision cancer therapy. Studying the effect of miRNA expression changes on apoptosis is important for evaluating miRNA-targeted therapy and realizing personalized precision therapy for cancer patients. Here, a new fluorescent nanoprobe was designed for the simultaneous imaging of miRNA-21 and apoptotic protein caspase-3 in cancer cells by using gold nanoparticles as the core and polydopamine as the shell. Confocal imaging indicated that the nanoprobe could be successfully applied for in situ monitoring of miRNA regulation of apoptosis. This design strategy is critical for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA, and developing personalized treatment plans.
format Online
Article
Text
id pubmed-9323288
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93232882022-07-27 Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe Ren, Mingyao Chen, Zhe Ge, Chuandong Hu, Wei Xu, Jing Yang, Limin Luan, Mingming Wang, Nianxing Pharmaceutics Article MiRNA-targeted therapy is an active research field in precision cancer therapy. Studying the effect of miRNA expression changes on apoptosis is important for evaluating miRNA-targeted therapy and realizing personalized precision therapy for cancer patients. Here, a new fluorescent nanoprobe was designed for the simultaneous imaging of miRNA-21 and apoptotic protein caspase-3 in cancer cells by using gold nanoparticles as the core and polydopamine as the shell. Confocal imaging indicated that the nanoprobe could be successfully applied for in situ monitoring of miRNA regulation of apoptosis. This design strategy is critical for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA, and developing personalized treatment plans. MDPI 2022-06-25 /pmc/articles/PMC9323288/ /pubmed/35890245 http://dx.doi.org/10.3390/pharmaceutics14071349 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ren, Mingyao
Chen, Zhe
Ge, Chuandong
Hu, Wei
Xu, Jing
Yang, Limin
Luan, Mingming
Wang, Nianxing
Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe
title Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe
title_full Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe
title_fullStr Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe
title_full_unstemmed Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe
title_short Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe
title_sort visualizing mirna regulation of apoptosis for investigating the feasibility of mirna-targeted therapy using a fluorescent nanoprobe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323288/
https://www.ncbi.nlm.nih.gov/pubmed/35890245
http://dx.doi.org/10.3390/pharmaceutics14071349
work_keys_str_mv AT renmingyao visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT chenzhe visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT gechuandong visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT huwei visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT xujing visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT yanglimin visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT luanmingming visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe
AT wangnianxing visualizingmirnaregulationofapoptosisforinvestigatingthefeasibilityofmirnatargetedtherapyusingafluorescentnanoprobe

Ejemplares similares