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Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation

The objectives of this research were to evaluate the policosanol profiles and adenosine-5′-monophosphate-activated protein kinase (AMPK) properties in the seedlings of Korean oat (Avena sativa L.) cultivars at different growth times. Nine policosanols in the silylated hexane extracts were detected u...

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Autores principales: Lee, Han-Gyeol, Woo, So-Yeun, Ahn, Hyung-Jae, Yang, Ji-Yeong, Lee, Mi-Ja, Kim, Hyun-Young, Song, Seung-Yeob, Lee, Jin-Hwan, Seo, Woo-Duck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323369/
https://www.ncbi.nlm.nih.gov/pubmed/35890478
http://dx.doi.org/10.3390/plants11141844
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author Lee, Han-Gyeol
Woo, So-Yeun
Ahn, Hyung-Jae
Yang, Ji-Yeong
Lee, Mi-Ja
Kim, Hyun-Young
Song, Seung-Yeob
Lee, Jin-Hwan
Seo, Woo-Duck
author_facet Lee, Han-Gyeol
Woo, So-Yeun
Ahn, Hyung-Jae
Yang, Ji-Yeong
Lee, Mi-Ja
Kim, Hyun-Young
Song, Seung-Yeob
Lee, Jin-Hwan
Seo, Woo-Duck
author_sort Lee, Han-Gyeol
collection PubMed
description The objectives of this research were to evaluate the policosanol profiles and adenosine-5′-monophosphate-activated protein kinase (AMPK) properties in the seedlings of Korean oat (Avena sativa L.) cultivars at different growth times. Nine policosanols in the silylated hexane extracts were detected using GC-MS and their contents showed considerable differences; specifically, hexacosanol (6) exhibited the highest composition, constituting 88–91% of the total average content. Moreover, the average hexacosanol (6) contents showed remarkable variations of 337.8 (5 days) → 416.8 (7 days) → 458.9 (9 days) → 490.0 (11 days) → 479.2 (13 days) → 427.0 mg/100 g (15 days). The seedlings collected at 11 days showed the highest average policosanol content (541.7 mg/100 g), with the lowest content being 383.4 mg/100 g after 5 days. Interestingly, policosanols from oat seedlings grown for 11 days induced the most prevalent phenotype of AMPK activation in HepG2 cells, indicating that policosanols are an excellent AMPK activator.
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spelling pubmed-93233692022-07-27 Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation Lee, Han-Gyeol Woo, So-Yeun Ahn, Hyung-Jae Yang, Ji-Yeong Lee, Mi-Ja Kim, Hyun-Young Song, Seung-Yeob Lee, Jin-Hwan Seo, Woo-Duck Plants (Basel) Article The objectives of this research were to evaluate the policosanol profiles and adenosine-5′-monophosphate-activated protein kinase (AMPK) properties in the seedlings of Korean oat (Avena sativa L.) cultivars at different growth times. Nine policosanols in the silylated hexane extracts were detected using GC-MS and their contents showed considerable differences; specifically, hexacosanol (6) exhibited the highest composition, constituting 88–91% of the total average content. Moreover, the average hexacosanol (6) contents showed remarkable variations of 337.8 (5 days) → 416.8 (7 days) → 458.9 (9 days) → 490.0 (11 days) → 479.2 (13 days) → 427.0 mg/100 g (15 days). The seedlings collected at 11 days showed the highest average policosanol content (541.7 mg/100 g), with the lowest content being 383.4 mg/100 g after 5 days. Interestingly, policosanols from oat seedlings grown for 11 days induced the most prevalent phenotype of AMPK activation in HepG2 cells, indicating that policosanols are an excellent AMPK activator. MDPI 2022-07-14 /pmc/articles/PMC9323369/ /pubmed/35890478 http://dx.doi.org/10.3390/plants11141844 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Han-Gyeol
Woo, So-Yeun
Ahn, Hyung-Jae
Yang, Ji-Yeong
Lee, Mi-Ja
Kim, Hyun-Young
Song, Seung-Yeob
Lee, Jin-Hwan
Seo, Woo-Duck
Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation
title Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation
title_full Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation
title_fullStr Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation
title_full_unstemmed Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation
title_short Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation
title_sort comparative analysis of policosanols related to growth times from the seedlings of various korean oat (avena sativa l.) cultivars and screening for adenosine 5′-monophosphate-activated protein kinase (ampk) activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323369/
https://www.ncbi.nlm.nih.gov/pubmed/35890478
http://dx.doi.org/10.3390/plants11141844
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