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Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal

Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competenc...

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Autores principales: Torrejón, Estefania, Sanches, Gustavo Seron, Moerbeck, Leonardo, Santos, Lenira, André, Marcos Rogério, Domingos, Ana, Antunes, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323395/
https://www.ncbi.nlm.nih.gov/pubmed/35889995
http://dx.doi.org/10.3390/pathogens11070749
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author Torrejón, Estefania
Sanches, Gustavo Seron
Moerbeck, Leonardo
Santos, Lenira
André, Marcos Rogério
Domingos, Ana
Antunes, Sandra
author_facet Torrejón, Estefania
Sanches, Gustavo Seron
Moerbeck, Leonardo
Santos, Lenira
André, Marcos Rogério
Domingos, Ana
Antunes, Sandra
author_sort Torrejón, Estefania
collection PubMed
description Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoG Bartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 10(5) copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats.
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spelling pubmed-93233952022-07-27 Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal Torrejón, Estefania Sanches, Gustavo Seron Moerbeck, Leonardo Santos, Lenira André, Marcos Rogério Domingos, Ana Antunes, Sandra Pathogens Article Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoG Bartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 10(5) copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats. MDPI 2022-06-30 /pmc/articles/PMC9323395/ /pubmed/35889995 http://dx.doi.org/10.3390/pathogens11070749 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Torrejón, Estefania
Sanches, Gustavo Seron
Moerbeck, Leonardo
Santos, Lenira
André, Marcos Rogério
Domingos, Ana
Antunes, Sandra
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
title Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
title_full Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
title_fullStr Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
title_full_unstemmed Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
title_short Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
title_sort molecular survey of bartonella species in stray cats and dogs, humans, and questing ticks from portugal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323395/
https://www.ncbi.nlm.nih.gov/pubmed/35889995
http://dx.doi.org/10.3390/pathogens11070749
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