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Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry
Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targ...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323402/ https://www.ncbi.nlm.nih.gov/pubmed/35889316 http://dx.doi.org/10.3390/molecules27144444 |
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author | Koulis, Georgios A. Tsagkaris, Aristeidis S. Katsianou, Panagiota A. Gialouris, Panagiotis-Loukas P. Martakos, Ioannis Stergiou, Fotis Fiore, Alberto Panagopoulou, Eleni I. Karabournioti, Sofia Baessmann, Carsten van der Borg, Noud Dasenaki, Marilena E. Proestos, Charalampos Thomaidis, Nikolaos S. |
author_facet | Koulis, Georgios A. Tsagkaris, Aristeidis S. Katsianou, Panagiota A. Gialouris, Panagiotis-Loukas P. Martakos, Ioannis Stergiou, Fotis Fiore, Alberto Panagopoulou, Eleni I. Karabournioti, Sofia Baessmann, Carsten van der Borg, Noud Dasenaki, Marilena E. Proestos, Charalampos Thomaidis, Nikolaos S. |
author_sort | Koulis, Georgios A. |
collection | PubMed |
description | Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches. |
format | Online Article Text |
id | pubmed-9323402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93234022022-07-27 Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry Koulis, Georgios A. Tsagkaris, Aristeidis S. Katsianou, Panagiota A. Gialouris, Panagiotis-Loukas P. Martakos, Ioannis Stergiou, Fotis Fiore, Alberto Panagopoulou, Eleni I. Karabournioti, Sofia Baessmann, Carsten van der Borg, Noud Dasenaki, Marilena E. Proestos, Charalampos Thomaidis, Nikolaos S. Molecules Article Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches. MDPI 2022-07-11 /pmc/articles/PMC9323402/ /pubmed/35889316 http://dx.doi.org/10.3390/molecules27144444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Koulis, Georgios A. Tsagkaris, Aristeidis S. Katsianou, Panagiota A. Gialouris, Panagiotis-Loukas P. Martakos, Ioannis Stergiou, Fotis Fiore, Alberto Panagopoulou, Eleni I. Karabournioti, Sofia Baessmann, Carsten van der Borg, Noud Dasenaki, Marilena E. Proestos, Charalampos Thomaidis, Nikolaos S. Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry |
title | Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry |
title_full | Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry |
title_fullStr | Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry |
title_full_unstemmed | Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry |
title_short | Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry |
title_sort | thorough investigation of the phenolic profile of reputable greek honey varieties: varietal discrimination and floral markers identification using liquid chromatography–high-resolution mass spectrometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323402/ https://www.ncbi.nlm.nih.gov/pubmed/35889316 http://dx.doi.org/10.3390/molecules27144444 |
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