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In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks

Adipocytes are energy stores of the body which also play a role in physiological regulation and homeostasis through their endocrine activity. Adipocyte circadian clocks drive rhythms in gene expression, and dysregulation of these circadian rhythms associates with pathological conditions such as diab...

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Autores principales: Martin, Capucine, Johnston, Jonathan D., Henslee, Erin A., van der Veen, Daan R., Labeed, Fatima H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323421/
https://www.ncbi.nlm.nih.gov/pubmed/35543378
http://dx.doi.org/10.1002/elps.202100308
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author Martin, Capucine
Johnston, Jonathan D.
Henslee, Erin A.
van der Veen, Daan R.
Labeed, Fatima H.
author_facet Martin, Capucine
Johnston, Jonathan D.
Henslee, Erin A.
van der Veen, Daan R.
Labeed, Fatima H.
author_sort Martin, Capucine
collection PubMed
description Adipocytes are energy stores of the body which also play a role in physiological regulation and homeostasis through their endocrine activity. Adipocyte circadian clocks drive rhythms in gene expression, and dysregulation of these circadian rhythms associates with pathological conditions such as diabetes. However, although the role of circadian rhythms in adipose cells and related tissues has been studied from phsyiological and molecular perspectives, they have not yet been explored from an electrical perspective. Research into electro‐chronobiology has revealed that electrical properties have important roles in peripheral clock regulation independently of transcription–translation feedback loops. We have used dielectrophoresis to study electrophysiological rhythms in pre‐adipocytes – representing an adipocyte precursor and nucleated cell‐based model, using serum shocking as the cellular method of clock entrainment. The results revealed significant electrophysiological rhythms, culminating in circadian (ca. 24 hourly) cycles in effective membrane capacitance and radius properties, whereas effective membrane conductance was observed to express ultradian (ca. 14 hourly) rhythms. These data shed new light into pre‐adipocyte electrical behaviour and present a potential target for understanding and manipulation of metabolic physiology.
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spelling pubmed-93234212022-07-30 In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks Martin, Capucine Johnston, Jonathan D. Henslee, Erin A. van der Veen, Daan R. Labeed, Fatima H. Electrophoresis Particle and Cell Analysis Adipocytes are energy stores of the body which also play a role in physiological regulation and homeostasis through their endocrine activity. Adipocyte circadian clocks drive rhythms in gene expression, and dysregulation of these circadian rhythms associates with pathological conditions such as diabetes. However, although the role of circadian rhythms in adipose cells and related tissues has been studied from phsyiological and molecular perspectives, they have not yet been explored from an electrical perspective. Research into electro‐chronobiology has revealed that electrical properties have important roles in peripheral clock regulation independently of transcription–translation feedback loops. We have used dielectrophoresis to study electrophysiological rhythms in pre‐adipocytes – representing an adipocyte precursor and nucleated cell‐based model, using serum shocking as the cellular method of clock entrainment. The results revealed significant electrophysiological rhythms, culminating in circadian (ca. 24 hourly) cycles in effective membrane capacitance and radius properties, whereas effective membrane conductance was observed to express ultradian (ca. 14 hourly) rhythms. These data shed new light into pre‐adipocyte electrical behaviour and present a potential target for understanding and manipulation of metabolic physiology. John Wiley and Sons Inc. 2022-05-22 2022-06 /pmc/articles/PMC9323421/ /pubmed/35543378 http://dx.doi.org/10.1002/elps.202100308 Text en © 2022 The Authors. Electrophoresis published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Particle and Cell Analysis
Martin, Capucine
Johnston, Jonathan D.
Henslee, Erin A.
van der Veen, Daan R.
Labeed, Fatima H.
In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
title In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
title_full In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
title_fullStr In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
title_full_unstemmed In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
title_short In vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
title_sort in vitro characterisation of murine pre‐adipose nucleated cells reveals electrophysiological cycles associated with biological clocks
topic Particle and Cell Analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323421/
https://www.ncbi.nlm.nih.gov/pubmed/35543378
http://dx.doi.org/10.1002/elps.202100308
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