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Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean

BACKGROUND: Herbicide‐resistant weeds pose a challenge to agriculture and food production. New herbicide tolerance traits in crops will provide farmers with more options to effectively manage weeds. Mesotrione, a selective pre‐ and post‐emergent triketone herbicide used in corn production, controls...

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Autores principales: Dai, Shunhong, Georgelis, Nikolaos, Bedair, Mohamed, Hong, Yun‐Jeong, Qi, Qungang, Larue, Clayton T, Sitoula, Bikram, Huang, Wei, Krebel, Brian, Shepard, Michael, Su, Wen, Kretzmer, Keith, Dong, Jiaxin, Slewinski, Thomas, Berger, Sarah, Ellis, Christine, Jerga, Agoston, Varagona, Marguerite
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323515/
https://www.ncbi.nlm.nih.gov/pubmed/35395133
http://dx.doi.org/10.1002/ps.6904
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author Dai, Shunhong
Georgelis, Nikolaos
Bedair, Mohamed
Hong, Yun‐Jeong
Qi, Qungang
Larue, Clayton T
Sitoula, Bikram
Huang, Wei
Krebel, Brian
Shepard, Michael
Su, Wen
Kretzmer, Keith
Dong, Jiaxin
Slewinski, Thomas
Berger, Sarah
Ellis, Christine
Jerga, Agoston
Varagona, Marguerite
author_facet Dai, Shunhong
Georgelis, Nikolaos
Bedair, Mohamed
Hong, Yun‐Jeong
Qi, Qungang
Larue, Clayton T
Sitoula, Bikram
Huang, Wei
Krebel, Brian
Shepard, Michael
Su, Wen
Kretzmer, Keith
Dong, Jiaxin
Slewinski, Thomas
Berger, Sarah
Ellis, Christine
Jerga, Agoston
Varagona, Marguerite
author_sort Dai, Shunhong
collection PubMed
description BACKGROUND: Herbicide‐resistant weeds pose a challenge to agriculture and food production. New herbicide tolerance traits in crops will provide farmers with more options to effectively manage weeds. Mesotrione, a selective pre‐ and post‐emergent triketone herbicide used in corn production, controls broadleaf and some annual grass weeds via hydroxyphenylpyruvate dioxygenase (HPPD) inhibition. Recently, the rice HIS1 gene, responsible for native tolerance to the selective triketone herbicide benzobicyclon, was identified. Expression of HIS1 also confers a modest level of mesotrione resistance in rice. Here we report the use of the HIS1 gene to develop a mesotrione tolerance trait in soybean. RESULTS: Conventional soybean is highly sensitive to mesotrione. Ectopic expression of a codon‐optimized version of the rice HIS1 gene (TDO) in soybean confers a commercial level of mesotrione tolerance. In TDO transgenic soybean plants, mesotrione is rapidly and locally oxidized into noninhibitory metabolites in leaf tissues directly exposed to the herbicide. These metabolites are further converted into compounds similar to known classes of plant secondary metabolites. This rapid metabolism prevents movement of mesotrione from treated leaves into vulnerable emerging leaves. Minimizing the accumulation of the herbicide in vulnerable emerging leaves protects the function of HPPD and carotenoid biosynthesis more generally while providing tolerance to mesotrione. CONCLUSIONS: Mesotrione has a favorable environmental and toxicological profile. The TDO‐mediated soybean mesotrione tolerance trait described here provides farmers with a new option to effectively manage difficult‐to‐control weeds using familiar herbicide chemistry. This trait can also be adapted to other mesotrione‐sensitive crops (e.g. cotton) for effective weed management. © 2022 Bayer Crop Science. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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spelling pubmed-93235152022-07-30 Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean Dai, Shunhong Georgelis, Nikolaos Bedair, Mohamed Hong, Yun‐Jeong Qi, Qungang Larue, Clayton T Sitoula, Bikram Huang, Wei Krebel, Brian Shepard, Michael Su, Wen Kretzmer, Keith Dong, Jiaxin Slewinski, Thomas Berger, Sarah Ellis, Christine Jerga, Agoston Varagona, Marguerite Pest Manag Sci Research Articles BACKGROUND: Herbicide‐resistant weeds pose a challenge to agriculture and food production. New herbicide tolerance traits in crops will provide farmers with more options to effectively manage weeds. Mesotrione, a selective pre‐ and post‐emergent triketone herbicide used in corn production, controls broadleaf and some annual grass weeds via hydroxyphenylpyruvate dioxygenase (HPPD) inhibition. Recently, the rice HIS1 gene, responsible for native tolerance to the selective triketone herbicide benzobicyclon, was identified. Expression of HIS1 also confers a modest level of mesotrione resistance in rice. Here we report the use of the HIS1 gene to develop a mesotrione tolerance trait in soybean. RESULTS: Conventional soybean is highly sensitive to mesotrione. Ectopic expression of a codon‐optimized version of the rice HIS1 gene (TDO) in soybean confers a commercial level of mesotrione tolerance. In TDO transgenic soybean plants, mesotrione is rapidly and locally oxidized into noninhibitory metabolites in leaf tissues directly exposed to the herbicide. These metabolites are further converted into compounds similar to known classes of plant secondary metabolites. This rapid metabolism prevents movement of mesotrione from treated leaves into vulnerable emerging leaves. Minimizing the accumulation of the herbicide in vulnerable emerging leaves protects the function of HPPD and carotenoid biosynthesis more generally while providing tolerance to mesotrione. CONCLUSIONS: Mesotrione has a favorable environmental and toxicological profile. The TDO‐mediated soybean mesotrione tolerance trait described here provides farmers with a new option to effectively manage difficult‐to‐control weeds using familiar herbicide chemistry. This trait can also be adapted to other mesotrione‐sensitive crops (e.g. cotton) for effective weed management. © 2022 Bayer Crop Science. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. John Wiley & Sons, Ltd. 2022-04-25 2022-07 /pmc/articles/PMC9323515/ /pubmed/35395133 http://dx.doi.org/10.1002/ps.6904 Text en © 2022 Bayer Crop Science. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Dai, Shunhong
Georgelis, Nikolaos
Bedair, Mohamed
Hong, Yun‐Jeong
Qi, Qungang
Larue, Clayton T
Sitoula, Bikram
Huang, Wei
Krebel, Brian
Shepard, Michael
Su, Wen
Kretzmer, Keith
Dong, Jiaxin
Slewinski, Thomas
Berger, Sarah
Ellis, Christine
Jerga, Agoston
Varagona, Marguerite
Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
title Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
title_full Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
title_fullStr Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
title_full_unstemmed Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
title_short Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
title_sort ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323515/
https://www.ncbi.nlm.nih.gov/pubmed/35395133
http://dx.doi.org/10.1002/ps.6904
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