Resistance and Pathogenicity of Salmonella Thompson Isolated from Incubation End of a Poultry Farm

SIMPLE SUMMARY: Non-typhoid Salmonella is the general term of Salmonella other than typhoid and paratyphoid, which often causes foodborne gastroenteritis in humans, but some serotypes have been proved to be pathogenic to poultry. Salmonella Enterica and Salmonella Typhimurium are the common serotypes pathogenic to poultry and have been systematically studied, but other serotypes have rarely been studied. During Salmonella surveillance in farms, we discovered by chance that Salmonella Thompson, a common non-typhoid Salmonella, is also pathogenic to avian embryos. Therefore, this study aimed to explore antimicrobial resistance and pathogenicity of clinical S. Thompson. Firstly, we found that the core-genome multilocus sequence typing of 14 clinical S. Thompson was consistent with two strains of S. Thompson from humans in China. Secondly, the antimicrobial resistance gene analysis demonstrated that all strains carried the polymyxin resistance gene mcr-9, which had not appeared resistance phenotype. Meanwhile, many essential virulence genes were also found in each S. Thompson isolate. Finally, the bacterial inoculation experiment revealed that clinical S. Thompson was highly pathogenic to newborn chicks after yolk sac inoculation. This study suggests that Salmonella Thompson can circulate between humans and poultry farms and transmit drug resistance genes and demonstrated that Salmonella Thompson is highly pathogenic to chicks and should be guarded against in the hatching stage of poultry farms. ABSTRACT: Salmonella Thompson, an important foodborne pathogen, is rarely found to be pathogenic to poultry. Accidentally, S. Thompson was found to be pathogenic to embryos of white feather broiler at a poultry farm in China. Therefore, this study aimed to explore antimicrobial resistance and pathogenicity of clinical S. Thompson isolated from dead poultry embryos. The phylogenetic tree based on 16S rRNA and seven housekeeping genes showed that the 14 clinical S. Thompson were closely related. The core-genome multilocus sequence typing of 14 clinical S. Thompson based on whole-genome sequencing was cgST-12774, consistent with the only two strains of S. Thompson from humans in China as reported in the NCBI database. The antimicrobial resistance gene analysis demonstrated that all strains carried aac(6′)-Iaa and the polymyxin resistance gene mcr-9. Antimicrobial sensitivity tests for 18 antibiotics showed that S. Thompson isolates displayed resistance against streptomycin (100%), ampicillin (35.7%), and doxycycline (14.3%), but sensitivity to polymyxin B, proving that the mcr-9 gene had not appeared resistance phenotype. Virulence genes Salmonella pathogenicity island (SPI) SPI1-5, type I fimbriae gene (fimA), flagellar assembly genes (bcfC, flhD, fliA, fliC, fljB, flgK, and lpfC), and other virulence genes (iroN, pagC, and cigR) were found in each S. Thompson isolate. Additionally, the bacterial inoculation experiment with 1-day-old chicks revealed that clinical S. Thompson was highly pathogenic to newborn chicks after yolk sac inoculation. This study highlighted that the S. Thompson isolated from poultry embryos and the S. Thompson causing human foodborne diarrhea in some parts of China belong to the same cgMLST typology (cgST-12774) and showed the pathogenicity of this clinical S. Thompson to chicks.