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Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels

The use of tobacco products is a major global public health issue, as it is the leading cause of preventable death worldwide. In addition, nicotine (NIC) is a key component of electronic and conventional cigarettes. Although nicotine’s addictive potential is well known, its health effects are not en...

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Autores principales: Chioran, Doina, Sitaru, Adrian, Macasoi, Ioana, Pinzaru, Iulia, Sarau, Cristian Andrei, Dehelean, Cristina, Dinu, Stefania, Szuhanek, Camelia, Zetu, Irina Nicoleta, Serafin, Andra Cristine, Rivis, Mircea, Poenaru, Marioara, Dragoi, Razvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323709/
https://www.ncbi.nlm.nih.gov/pubmed/35886732
http://dx.doi.org/10.3390/ijerph19148881
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author Chioran, Doina
Sitaru, Adrian
Macasoi, Ioana
Pinzaru, Iulia
Sarau, Cristian Andrei
Dehelean, Cristina
Dinu, Stefania
Szuhanek, Camelia
Zetu, Irina Nicoleta
Serafin, Andra Cristine
Rivis, Mircea
Poenaru, Marioara
Dragoi, Razvan
author_facet Chioran, Doina
Sitaru, Adrian
Macasoi, Ioana
Pinzaru, Iulia
Sarau, Cristian Andrei
Dehelean, Cristina
Dinu, Stefania
Szuhanek, Camelia
Zetu, Irina Nicoleta
Serafin, Andra Cristine
Rivis, Mircea
Poenaru, Marioara
Dragoi, Razvan
author_sort Chioran, Doina
collection PubMed
description The use of tobacco products is a major global public health issue, as it is the leading cause of preventable death worldwide. In addition, nicotine (NIC) is a key component of electronic and conventional cigarettes. Although nicotine’s addictive potential is well known, its health effects are not entirely understood. Thus, the main objective of the present study was to evaluate its toxicological profile both in vitro, at the level of three healthy cell lines, and in ovo, at the level of the chorioallantoic membrane. Five different concentrations of nicotine were used in keratinocytes, cardiomyocytes, and hepatocytes for the purpose of evaluating cell viability, cell morphology, and its impact on nuclei. Additionally, the hen’s egg test on the chorioallantoic membrane (HET-CAM) method was used to assess the biocompatibility and irritant potential of the chorioallantoic membrane. Across all cell lines studied, nicotine was proven to be significantly damaging to cell viability, with the highest concentration tested resulting in less than 2% viable cells. Moreover, the morphology of cells changed dramatically, with alterations in their shape and confluence. Nicotine-induced cell death appears to be apoptotic, based on its impact on the nucleus. In addition, nicotine was also found to have a very strong irritating effect on the chorioallantoic membrane. In conclusion, nicotine has an extremely strong toxicological profile, as demonstrated by the drastic reduction of cell viability and the induction of morphological changes and nuclear alterations associated with cellular apoptosis. Additionally, the HET-CAM method led to the observation of a strong irritating effect associated with nicotine.
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spelling pubmed-93237092022-07-27 Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels Chioran, Doina Sitaru, Adrian Macasoi, Ioana Pinzaru, Iulia Sarau, Cristian Andrei Dehelean, Cristina Dinu, Stefania Szuhanek, Camelia Zetu, Irina Nicoleta Serafin, Andra Cristine Rivis, Mircea Poenaru, Marioara Dragoi, Razvan Int J Environ Res Public Health Article The use of tobacco products is a major global public health issue, as it is the leading cause of preventable death worldwide. In addition, nicotine (NIC) is a key component of electronic and conventional cigarettes. Although nicotine’s addictive potential is well known, its health effects are not entirely understood. Thus, the main objective of the present study was to evaluate its toxicological profile both in vitro, at the level of three healthy cell lines, and in ovo, at the level of the chorioallantoic membrane. Five different concentrations of nicotine were used in keratinocytes, cardiomyocytes, and hepatocytes for the purpose of evaluating cell viability, cell morphology, and its impact on nuclei. Additionally, the hen’s egg test on the chorioallantoic membrane (HET-CAM) method was used to assess the biocompatibility and irritant potential of the chorioallantoic membrane. Across all cell lines studied, nicotine was proven to be significantly damaging to cell viability, with the highest concentration tested resulting in less than 2% viable cells. Moreover, the morphology of cells changed dramatically, with alterations in their shape and confluence. Nicotine-induced cell death appears to be apoptotic, based on its impact on the nucleus. In addition, nicotine was also found to have a very strong irritating effect on the chorioallantoic membrane. In conclusion, nicotine has an extremely strong toxicological profile, as demonstrated by the drastic reduction of cell viability and the induction of morphological changes and nuclear alterations associated with cellular apoptosis. Additionally, the HET-CAM method led to the observation of a strong irritating effect associated with nicotine. MDPI 2022-07-21 /pmc/articles/PMC9323709/ /pubmed/35886732 http://dx.doi.org/10.3390/ijerph19148881 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chioran, Doina
Sitaru, Adrian
Macasoi, Ioana
Pinzaru, Iulia
Sarau, Cristian Andrei
Dehelean, Cristina
Dinu, Stefania
Szuhanek, Camelia
Zetu, Irina Nicoleta
Serafin, Andra Cristine
Rivis, Mircea
Poenaru, Marioara
Dragoi, Razvan
Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels
title Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels
title_full Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels
title_fullStr Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels
title_full_unstemmed Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels
title_short Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels
title_sort nicotine exerts cytotoxic effects in a panel of healthy cell lines and strong irritating potential on blood vessels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323709/
https://www.ncbi.nlm.nih.gov/pubmed/35886732
http://dx.doi.org/10.3390/ijerph19148881
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