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The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis

Worldwide, poultry infections by Salmonella are the cause of significant economic losses, not only due to reduced production (due to fowl typhoid disease), but also considering the efforts and control measures that must be constantly applied, especially due to zoonotic serovars. Poultry is a common...

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Autores principales: Huberman, Yosef Daniel, Caballero-García, Melanie, Rojas, Rober, Ascanio, Silvia, Olmos, Leandro Hipólito, Malena, Rosana, Lomónaco, Jorgelina, Nievas, Paula, Chero, Paula, Lévano-Gracía, Julio, Mendoza-Espinoza, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323758/
https://www.ncbi.nlm.nih.gov/pubmed/35891276
http://dx.doi.org/10.3390/vaccines10071113
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author Huberman, Yosef Daniel
Caballero-García, Melanie
Rojas, Rober
Ascanio, Silvia
Olmos, Leandro Hipólito
Malena, Rosana
Lomónaco, Jorgelina
Nievas, Paula
Chero, Paula
Lévano-Gracía, Julio
Mendoza-Espinoza, Alfredo
author_facet Huberman, Yosef Daniel
Caballero-García, Melanie
Rojas, Rober
Ascanio, Silvia
Olmos, Leandro Hipólito
Malena, Rosana
Lomónaco, Jorgelina
Nievas, Paula
Chero, Paula
Lévano-Gracía, Julio
Mendoza-Espinoza, Alfredo
author_sort Huberman, Yosef Daniel
collection PubMed
description Worldwide, poultry infections by Salmonella are the cause of significant economic losses, not only due to reduced production (due to fowl typhoid disease), but also considering the efforts and control measures that must be constantly applied, especially due to zoonotic serovars. Poultry is a common reservoir of Salmonella and its transmission into the food chain is a risk for humans. The vaccination of layers plays an important role in the overall efforts to prevent Salmonella infections. An inactivated trivalent vaccine was prepared with S. Enteritidis, S. Typhimurium, and S. Infantis strains. Infection trials were performed to evaluate the efficacy of three vaccination schedules using inactivated and live S. Gallinarum 9R vaccines. For this purpose, at week 5 of life, one subcutaneous dose of live S. Gallinarum 9R vaccine (1–5 × 10(7) CFU) was given to Groups 1 and 2. At weeks 8 and 11 of life, chickens were also vaccinated with one (Group 1) or two (Groups 2 and 3) intramuscular doses of the inactivated oil-adjuvant trivalent vaccine (1 × 10(8) CFU/dose of each antigen). Group 4 consisted of chickens that remained unvaccinated (control). At week 14 of life, the efficacy of the vaccination plans was evaluated in three separate inoculation trials with S. Enteritidis, S. Typhimurium, or S. Infantis. After vaccination with the inactivated vaccine, homologous antibody production was observed, and after challenge, a significant reduction in the faecal shedding, invasion, and colonization of S. Typhimurium and S. Infantis was achieved by all vaccination schedules, while the vaccination with at least one dose of the live S. Gallinarum 9R vaccine was necessary to obtain such a significant protection against S. Enteritidis infection.
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spelling pubmed-93237582022-07-27 The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis Huberman, Yosef Daniel Caballero-García, Melanie Rojas, Rober Ascanio, Silvia Olmos, Leandro Hipólito Malena, Rosana Lomónaco, Jorgelina Nievas, Paula Chero, Paula Lévano-Gracía, Julio Mendoza-Espinoza, Alfredo Vaccines (Basel) Article Worldwide, poultry infections by Salmonella are the cause of significant economic losses, not only due to reduced production (due to fowl typhoid disease), but also considering the efforts and control measures that must be constantly applied, especially due to zoonotic serovars. Poultry is a common reservoir of Salmonella and its transmission into the food chain is a risk for humans. The vaccination of layers plays an important role in the overall efforts to prevent Salmonella infections. An inactivated trivalent vaccine was prepared with S. Enteritidis, S. Typhimurium, and S. Infantis strains. Infection trials were performed to evaluate the efficacy of three vaccination schedules using inactivated and live S. Gallinarum 9R vaccines. For this purpose, at week 5 of life, one subcutaneous dose of live S. Gallinarum 9R vaccine (1–5 × 10(7) CFU) was given to Groups 1 and 2. At weeks 8 and 11 of life, chickens were also vaccinated with one (Group 1) or two (Groups 2 and 3) intramuscular doses of the inactivated oil-adjuvant trivalent vaccine (1 × 10(8) CFU/dose of each antigen). Group 4 consisted of chickens that remained unvaccinated (control). At week 14 of life, the efficacy of the vaccination plans was evaluated in three separate inoculation trials with S. Enteritidis, S. Typhimurium, or S. Infantis. After vaccination with the inactivated vaccine, homologous antibody production was observed, and after challenge, a significant reduction in the faecal shedding, invasion, and colonization of S. Typhimurium and S. Infantis was achieved by all vaccination schedules, while the vaccination with at least one dose of the live S. Gallinarum 9R vaccine was necessary to obtain such a significant protection against S. Enteritidis infection. MDPI 2022-07-12 /pmc/articles/PMC9323758/ /pubmed/35891276 http://dx.doi.org/10.3390/vaccines10071113 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huberman, Yosef Daniel
Caballero-García, Melanie
Rojas, Rober
Ascanio, Silvia
Olmos, Leandro Hipólito
Malena, Rosana
Lomónaco, Jorgelina
Nievas, Paula
Chero, Paula
Lévano-Gracía, Julio
Mendoza-Espinoza, Alfredo
The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis
title The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis
title_full The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis
title_fullStr The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis
title_full_unstemmed The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis
title_short The Efficacy of a Trivalent Inactivated Salmonella Vaccine Combined with the Live S. Gallinarum 9R Vaccine in Young Layers after Experimental Infections with S. Enteritidis, S. Typhimurium, and S. Infantis
title_sort efficacy of a trivalent inactivated salmonella vaccine combined with the live s. gallinarum 9r vaccine in young layers after experimental infections with s. enteritidis, s. typhimurium, and s. infantis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323758/
https://www.ncbi.nlm.nih.gov/pubmed/35891276
http://dx.doi.org/10.3390/vaccines10071113
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