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Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes

Ixodes ricinus and Ixodes persulcatus ticks are the main vectors of tick-borne encephalitis virus and some bacterial pathogens. The regions where these tick species live overlap, forming large sympatric areas. It has previously been shown that these tick species have no morphological barrier, and in...

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Autores principales: Litov, Alexander G., Belova, Oxana A., Bugmyrin, Sergey V., Kholodilov, Ivan S., Romanova, Lidia Iu., Karganova, Galina G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323786/
https://www.ncbi.nlm.nih.gov/pubmed/35889025
http://dx.doi.org/10.3390/microorganisms10071306
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author Litov, Alexander G.
Belova, Oxana A.
Bugmyrin, Sergey V.
Kholodilov, Ivan S.
Romanova, Lidia Iu.
Karganova, Galina G.
author_facet Litov, Alexander G.
Belova, Oxana A.
Bugmyrin, Sergey V.
Kholodilov, Ivan S.
Romanova, Lidia Iu.
Karganova, Galina G.
author_sort Litov, Alexander G.
collection PubMed
description Ixodes ricinus and Ixodes persulcatus ticks are the main vectors of tick-borne encephalitis virus and some bacterial pathogens. The regions where these tick species live overlap, forming large sympatric areas. It has previously been shown that these tick species have no morphological barrier, and interspecies crossing is possible with the appearance of sterile hybrids. It has also been shown that hybrid larvae and nymphs can be differentiated using discriminant functions based on a set of morphological features. However, such an approach is laborious and rather ineffective with adult ticks, making a molecular approach necessary. In the current work, we tested the ability of different systems to differentiate laboratory-obtained hybrid ticks. Our data suggest that commonly used primer sets that target rRNA are unsuitable for hybrid tick determination, likely due to the rRNA region being linked with the X chromosome in I. ricinus and I. persulcatus ticks. We tested several primer sets targeting different non rRNA genes to assess their ability to determine hybrids. The best primer set, Toll_R, targeting the putative Toll gene, showed little to no bias when used for DNA amplification from hybrid ticks. Thus, Toll gene can be further used for hybrid detection.
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spelling pubmed-93237862022-07-27 Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes Litov, Alexander G. Belova, Oxana A. Bugmyrin, Sergey V. Kholodilov, Ivan S. Romanova, Lidia Iu. Karganova, Galina G. Microorganisms Article Ixodes ricinus and Ixodes persulcatus ticks are the main vectors of tick-borne encephalitis virus and some bacterial pathogens. The regions where these tick species live overlap, forming large sympatric areas. It has previously been shown that these tick species have no morphological barrier, and interspecies crossing is possible with the appearance of sterile hybrids. It has also been shown that hybrid larvae and nymphs can be differentiated using discriminant functions based on a set of morphological features. However, such an approach is laborious and rather ineffective with adult ticks, making a molecular approach necessary. In the current work, we tested the ability of different systems to differentiate laboratory-obtained hybrid ticks. Our data suggest that commonly used primer sets that target rRNA are unsuitable for hybrid tick determination, likely due to the rRNA region being linked with the X chromosome in I. ricinus and I. persulcatus ticks. We tested several primer sets targeting different non rRNA genes to assess their ability to determine hybrids. The best primer set, Toll_R, targeting the putative Toll gene, showed little to no bias when used for DNA amplification from hybrid ticks. Thus, Toll gene can be further used for hybrid detection. MDPI 2022-06-27 /pmc/articles/PMC9323786/ /pubmed/35889025 http://dx.doi.org/10.3390/microorganisms10071306 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Litov, Alexander G.
Belova, Oxana A.
Bugmyrin, Sergey V.
Kholodilov, Ivan S.
Romanova, Lidia Iu.
Karganova, Galina G.
Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes
title Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes
title_full Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes
title_fullStr Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes
title_full_unstemmed Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes
title_short Differentiation of Laboratory-Obtained Ixodes ricinus × Ixodes persulcatus Hybrid Ticks: Selection of Suitable Genes
title_sort differentiation of laboratory-obtained ixodes ricinus × ixodes persulcatus hybrid ticks: selection of suitable genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323786/
https://www.ncbi.nlm.nih.gov/pubmed/35889025
http://dx.doi.org/10.3390/microorganisms10071306
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