Cargando…
Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells
Articaine (ATC) and lidocaine (LDC) are the local anesthetics (LAs) currently most employed in dentistry. Cases of paresthesia, reported more frequently for ATC, have raised concerns about their potential neurotoxicity, calling for further investigation of their biological effects in neuronal cells....
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323911/ https://www.ncbi.nlm.nih.gov/pubmed/35888705 http://dx.doi.org/10.3390/metabo12070581 |
_version_ | 1784756674747170816 |
---|---|
author | da Silva, Gustavo H. Rodrigues Mendes, Luís F. de Carvalho, Fabíola V. de Paula, Eneida Duarte, Iola F. |
author_facet | da Silva, Gustavo H. Rodrigues Mendes, Luís F. de Carvalho, Fabíola V. de Paula, Eneida Duarte, Iola F. |
author_sort | da Silva, Gustavo H. Rodrigues |
collection | PubMed |
description | Articaine (ATC) and lidocaine (LDC) are the local anesthetics (LAs) currently most employed in dentistry. Cases of paresthesia, reported more frequently for ATC, have raised concerns about their potential neurotoxicity, calling for further investigation of their biological effects in neuronal cells. In this work, the impact of ATC and LDC on the metabolism of SH-SY5Y cells was investigated through (1)H NMR metabolomics. For each LA, in vitro cultured cells were exposed to concentrations causing 10 and 50% reductions in cell viability, and their metabolic intracellular and extracellular profiles were characterized. Most effects were common to ATC and LDC, although with varying magnitudes. The metabolic variations elicited by the two LAs suggested (i) downregulation of glycolysis and of glucose-dependent pathways (e.g., one-carbon metabolism and hexosamine biosynthetic pathway), (ii) disturbance of branched chain amino acids (BCAA) catabolism, (iii) downregulation of TCA cycle anaplerotic fueling and activation of alternative energy producing pathways, (iv) interference with choline metabolism and (v) lipid droplet build-up. Interestingly, LDC had a greater impact on membrane phospholipid turnover, as suggested by higher phosphatidylcholine to phosphocholine conversion. Moreover, LDC elicited an increase in triglycerides, whereas cholesteryl esters accumulated in ATC-exposed cells, suggesting a different composition and handling of lipid droplets. |
format | Online Article Text |
id | pubmed-9323911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93239112022-07-27 Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells da Silva, Gustavo H. Rodrigues Mendes, Luís F. de Carvalho, Fabíola V. de Paula, Eneida Duarte, Iola F. Metabolites Article Articaine (ATC) and lidocaine (LDC) are the local anesthetics (LAs) currently most employed in dentistry. Cases of paresthesia, reported more frequently for ATC, have raised concerns about their potential neurotoxicity, calling for further investigation of their biological effects in neuronal cells. In this work, the impact of ATC and LDC on the metabolism of SH-SY5Y cells was investigated through (1)H NMR metabolomics. For each LA, in vitro cultured cells were exposed to concentrations causing 10 and 50% reductions in cell viability, and their metabolic intracellular and extracellular profiles were characterized. Most effects were common to ATC and LDC, although with varying magnitudes. The metabolic variations elicited by the two LAs suggested (i) downregulation of glycolysis and of glucose-dependent pathways (e.g., one-carbon metabolism and hexosamine biosynthetic pathway), (ii) disturbance of branched chain amino acids (BCAA) catabolism, (iii) downregulation of TCA cycle anaplerotic fueling and activation of alternative energy producing pathways, (iv) interference with choline metabolism and (v) lipid droplet build-up. Interestingly, LDC had a greater impact on membrane phospholipid turnover, as suggested by higher phosphatidylcholine to phosphocholine conversion. Moreover, LDC elicited an increase in triglycerides, whereas cholesteryl esters accumulated in ATC-exposed cells, suggesting a different composition and handling of lipid droplets. MDPI 2022-06-23 /pmc/articles/PMC9323911/ /pubmed/35888705 http://dx.doi.org/10.3390/metabo12070581 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article da Silva, Gustavo H. Rodrigues Mendes, Luís F. de Carvalho, Fabíola V. de Paula, Eneida Duarte, Iola F. Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells |
title | Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells |
title_full | Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells |
title_fullStr | Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells |
title_full_unstemmed | Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells |
title_short | Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells |
title_sort | comparative metabolomics study of the impact of articaine and lidocaine on the metabolism of sh-sy5y neuronal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323911/ https://www.ncbi.nlm.nih.gov/pubmed/35888705 http://dx.doi.org/10.3390/metabo12070581 |
work_keys_str_mv | AT dasilvagustavohrodrigues comparativemetabolomicsstudyoftheimpactofarticaineandlidocaineonthemetabolismofshsy5yneuronalcells AT mendesluisf comparativemetabolomicsstudyoftheimpactofarticaineandlidocaineonthemetabolismofshsy5yneuronalcells AT decarvalhofabiolav comparativemetabolomicsstudyoftheimpactofarticaineandlidocaineonthemetabolismofshsy5yneuronalcells AT depaulaeneida comparativemetabolomicsstudyoftheimpactofarticaineandlidocaineonthemetabolismofshsy5yneuronalcells AT duarteiolaf comparativemetabolomicsstudyoftheimpactofarticaineandlidocaineonthemetabolismofshsy5yneuronalcells |