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Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes

Magnetic levitation (MagLev) has recently emerged as a powerful method to develop diagnostic technologies based on the exploitation of the nanoparticle (NP)–protein corona. However, experimental procedures improving the robustness, reproducibility, and accuracy of this technology are largely unexplo...

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Autores principales: Digiacomo, Luca, Quagliarini, Erica, Marmiroli, Benedetta, Sartori, Barbara, Perini, Giordano, Papi, Massimiliano, Capriotti, Anna Laura, Montone, Carmela Maria, Cerrato, Andrea, Caracciolo, Giulio, Pozzi, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324036/
https://www.ncbi.nlm.nih.gov/pubmed/35889600
http://dx.doi.org/10.3390/nano12142376
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author Digiacomo, Luca
Quagliarini, Erica
Marmiroli, Benedetta
Sartori, Barbara
Perini, Giordano
Papi, Massimiliano
Capriotti, Anna Laura
Montone, Carmela Maria
Cerrato, Andrea
Caracciolo, Giulio
Pozzi, Daniela
author_facet Digiacomo, Luca
Quagliarini, Erica
Marmiroli, Benedetta
Sartori, Barbara
Perini, Giordano
Papi, Massimiliano
Capriotti, Anna Laura
Montone, Carmela Maria
Cerrato, Andrea
Caracciolo, Giulio
Pozzi, Daniela
author_sort Digiacomo, Luca
collection PubMed
description Magnetic levitation (MagLev) has recently emerged as a powerful method to develop diagnostic technologies based on the exploitation of the nanoparticle (NP)–protein corona. However, experimental procedures improving the robustness, reproducibility, and accuracy of this technology are largely unexplored. To contribute to filling this gap, here, we investigated the effect of total flow rate (TFR) and flow rate ratio (FRR) on the MagLev patterns of microfluidic-generated graphene oxide (GO)–protein complexes using bulk mixing of GO and human plasma (HP) as a reference. Levitating and precipitating fractions of GO-HP samples were characterized in terms of atomic force microscopy (AFM), bicinchoninic acid assay (BCA), and one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (1D SDS-PAGE), and nanoliquid chromatography–tandem mass spectrometry (nano-LC-MS/MS). We identified combinations of TFR and FRR (e.g., TFR = 35 μL/min and FRR (GO:HP) = 9:1 or TFR = 3.5 μL/min and FRR (GO:HP) = 19:1), leading to MagLev patterns dominated by levitating and precipitating fractions with bulk-like features. Since a typical MagLev experiment for disease detection is based on a sequence of optimization, exploration, and validation steps, this implies that the optimization (e.g., searching for optimal NP:HP ratios) and exploration (e.g., searching for MagLev signatures) steps can be performed using samples generated by bulk mixing. When these steps are completed, the validation step, which involves using human specimens that are often available in limited amounts, can be made by highly reproducible microfluidic mixing without any ex novo optimization process. The relevance of developing diagnostic technologies based on MagLev of coronated nanomaterials is also discussed.
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spelling pubmed-93240362022-07-27 Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes Digiacomo, Luca Quagliarini, Erica Marmiroli, Benedetta Sartori, Barbara Perini, Giordano Papi, Massimiliano Capriotti, Anna Laura Montone, Carmela Maria Cerrato, Andrea Caracciolo, Giulio Pozzi, Daniela Nanomaterials (Basel) Article Magnetic levitation (MagLev) has recently emerged as a powerful method to develop diagnostic technologies based on the exploitation of the nanoparticle (NP)–protein corona. However, experimental procedures improving the robustness, reproducibility, and accuracy of this technology are largely unexplored. To contribute to filling this gap, here, we investigated the effect of total flow rate (TFR) and flow rate ratio (FRR) on the MagLev patterns of microfluidic-generated graphene oxide (GO)–protein complexes using bulk mixing of GO and human plasma (HP) as a reference. Levitating and precipitating fractions of GO-HP samples were characterized in terms of atomic force microscopy (AFM), bicinchoninic acid assay (BCA), and one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (1D SDS-PAGE), and nanoliquid chromatography–tandem mass spectrometry (nano-LC-MS/MS). We identified combinations of TFR and FRR (e.g., TFR = 35 μL/min and FRR (GO:HP) = 9:1 or TFR = 3.5 μL/min and FRR (GO:HP) = 19:1), leading to MagLev patterns dominated by levitating and precipitating fractions with bulk-like features. Since a typical MagLev experiment for disease detection is based on a sequence of optimization, exploration, and validation steps, this implies that the optimization (e.g., searching for optimal NP:HP ratios) and exploration (e.g., searching for MagLev signatures) steps can be performed using samples generated by bulk mixing. When these steps are completed, the validation step, which involves using human specimens that are often available in limited amounts, can be made by highly reproducible microfluidic mixing without any ex novo optimization process. The relevance of developing diagnostic technologies based on MagLev of coronated nanomaterials is also discussed. MDPI 2022-07-11 /pmc/articles/PMC9324036/ /pubmed/35889600 http://dx.doi.org/10.3390/nano12142376 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Digiacomo, Luca
Quagliarini, Erica
Marmiroli, Benedetta
Sartori, Barbara
Perini, Giordano
Papi, Massimiliano
Capriotti, Anna Laura
Montone, Carmela Maria
Cerrato, Andrea
Caracciolo, Giulio
Pozzi, Daniela
Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
title Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
title_full Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
title_fullStr Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
title_full_unstemmed Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
title_short Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
title_sort magnetic levitation patterns of microfluidic-generated nanoparticle–protein complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324036/
https://www.ncbi.nlm.nih.gov/pubmed/35889600
http://dx.doi.org/10.3390/nano12142376
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