Cargando…

The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley

The barley cellulose synthase‐like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Lou, Haoyu, Tucker, Matthew R., Shirley, Neil J., Lahnstein, Jelle, Yang, Xiujuan, Ma, Chao, Schwerdt, Julian, Fusi, Riccardo, Burton, Rachel A., Band, Leah R., Bennett, Malcolm J., Bulone, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324092/
https://www.ncbi.nlm.nih.gov/pubmed/35395116
http://dx.doi.org/10.1111/tpj.15764
_version_ 1784756722109251584
author Lou, Haoyu
Tucker, Matthew R.
Shirley, Neil J.
Lahnstein, Jelle
Yang, Xiujuan
Ma, Chao
Schwerdt, Julian
Fusi, Riccardo
Burton, Rachel A.
Band, Leah R.
Bennett, Malcolm J.
Bulone, Vincent
author_facet Lou, Haoyu
Tucker, Matthew R.
Shirley, Neil J.
Lahnstein, Jelle
Yang, Xiujuan
Ma, Chao
Schwerdt, Julian
Fusi, Riccardo
Burton, Rachel A.
Band, Leah R.
Bennett, Malcolm J.
Bulone, Vincent
author_sort Lou, Haoyu
collection PubMed
description The barley cellulose synthase‐like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 (HvCslF3) gene was shown to be involved in the synthesis of a novel (1,4)‐β‐linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT‐PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1,4)‐β‐linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild‐type (Col‐0) and root hair‐deficient Arabidopsis mutants (csld3 and csld5) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation.
format Online
Article
Text
id pubmed-9324092
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-93240922022-07-30 The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley Lou, Haoyu Tucker, Matthew R. Shirley, Neil J. Lahnstein, Jelle Yang, Xiujuan Ma, Chao Schwerdt, Julian Fusi, Riccardo Burton, Rachel A. Band, Leah R. Bennett, Malcolm J. Bulone, Vincent Plant J Original Articles The barley cellulose synthase‐like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 (HvCslF3) gene was shown to be involved in the synthesis of a novel (1,4)‐β‐linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT‐PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1,4)‐β‐linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild‐type (Col‐0) and root hair‐deficient Arabidopsis mutants (csld3 and csld5) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation. John Wiley and Sons Inc. 2022-05-04 2022-06 /pmc/articles/PMC9324092/ /pubmed/35395116 http://dx.doi.org/10.1111/tpj.15764 Text en © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Lou, Haoyu
Tucker, Matthew R.
Shirley, Neil J.
Lahnstein, Jelle
Yang, Xiujuan
Ma, Chao
Schwerdt, Julian
Fusi, Riccardo
Burton, Rachel A.
Band, Leah R.
Bennett, Malcolm J.
Bulone, Vincent
The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
title The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
title_full The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
title_fullStr The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
title_full_unstemmed The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
title_short The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
title_sort cellulose synthase‐like f3 (cslf3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324092/
https://www.ncbi.nlm.nih.gov/pubmed/35395116
http://dx.doi.org/10.1111/tpj.15764
work_keys_str_mv AT louhaoyu thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT tuckermatthewr thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT shirleyneilj thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT lahnsteinjelle thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT yangxiujuan thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT machao thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT schwerdtjulian thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT fusiriccardo thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT burtonrachela thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT bandleahr thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT bennettmalcolmj thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT bulonevincent thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT louhaoyu cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT tuckermatthewr cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT shirleyneilj cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT lahnsteinjelle cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT yangxiujuan cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT machao cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT schwerdtjulian cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT fusiriccardo cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT burtonrachela cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT bandleahr cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT bennettmalcolmj cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley
AT bulonevincent cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley