Cargando…
The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley
The barley cellulose synthase‐like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, ma...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324092/ https://www.ncbi.nlm.nih.gov/pubmed/35395116 http://dx.doi.org/10.1111/tpj.15764 |
_version_ | 1784756722109251584 |
---|---|
author | Lou, Haoyu Tucker, Matthew R. Shirley, Neil J. Lahnstein, Jelle Yang, Xiujuan Ma, Chao Schwerdt, Julian Fusi, Riccardo Burton, Rachel A. Band, Leah R. Bennett, Malcolm J. Bulone, Vincent |
author_facet | Lou, Haoyu Tucker, Matthew R. Shirley, Neil J. Lahnstein, Jelle Yang, Xiujuan Ma, Chao Schwerdt, Julian Fusi, Riccardo Burton, Rachel A. Band, Leah R. Bennett, Malcolm J. Bulone, Vincent |
author_sort | Lou, Haoyu |
collection | PubMed |
description | The barley cellulose synthase‐like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 (HvCslF3) gene was shown to be involved in the synthesis of a novel (1,4)‐β‐linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT‐PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1,4)‐β‐linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild‐type (Col‐0) and root hair‐deficient Arabidopsis mutants (csld3 and csld5) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation. |
format | Online Article Text |
id | pubmed-9324092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93240922022-07-30 The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley Lou, Haoyu Tucker, Matthew R. Shirley, Neil J. Lahnstein, Jelle Yang, Xiujuan Ma, Chao Schwerdt, Julian Fusi, Riccardo Burton, Rachel A. Band, Leah R. Bennett, Malcolm J. Bulone, Vincent Plant J Original Articles The barley cellulose synthase‐like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 (HvCslF3) gene was shown to be involved in the synthesis of a novel (1,4)‐β‐linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT‐PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1,4)‐β‐linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild‐type (Col‐0) and root hair‐deficient Arabidopsis mutants (csld3 and csld5) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation. John Wiley and Sons Inc. 2022-05-04 2022-06 /pmc/articles/PMC9324092/ /pubmed/35395116 http://dx.doi.org/10.1111/tpj.15764 Text en © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Lou, Haoyu Tucker, Matthew R. Shirley, Neil J. Lahnstein, Jelle Yang, Xiujuan Ma, Chao Schwerdt, Julian Fusi, Riccardo Burton, Rachel A. Band, Leah R. Bennett, Malcolm J. Bulone, Vincent The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
title | The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
title_full | The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
title_fullStr | The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
title_full_unstemmed | The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
title_short | The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
title_sort | cellulose synthase‐like f3 (cslf3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324092/ https://www.ncbi.nlm.nih.gov/pubmed/35395116 http://dx.doi.org/10.1111/tpj.15764 |
work_keys_str_mv | AT louhaoyu thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT tuckermatthewr thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT shirleyneilj thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT lahnsteinjelle thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT yangxiujuan thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT machao thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT schwerdtjulian thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT fusiriccardo thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT burtonrachela thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT bandleahr thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT bennettmalcolmj thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT bulonevincent thecellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT louhaoyu cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT tuckermatthewr cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT shirleyneilj cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT lahnsteinjelle cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT yangxiujuan cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT machao cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT schwerdtjulian cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT fusiriccardo cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT burtonrachela cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT bandleahr cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT bennettmalcolmj cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley AT bulonevincent cellulosesynthaselikef3cslf3genemediatescellwallpolysaccharidesynthesisandaffectsrootgrowthanddifferentiationinbarley |