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Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrod...

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Detalles Bibliográficos
Autores principales: Wei, Zheng, Cai, Xiaoping, Cui, Weifeng, Zhang, Junping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324264/
https://www.ncbi.nlm.nih.gov/pubmed/35889454
http://dx.doi.org/10.3390/molecules27144578
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author Wei, Zheng
Cai, Xiaoping
Cui, Weifeng
Zhang, Junping
author_facet Wei, Zheng
Cai, Xiaoping
Cui, Weifeng
Zhang, Junping
author_sort Wei, Zheng
collection PubMed
description A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrodes were placed in a 3-mercaptopropionic acid (MPA)/β-mercaptoethanol (ME) mixture for 24 h for self-assembly. The electrodes were then placed in an EDC/NHS mixture for 1 h. Layer modification was performed by stepwise dropwise addition of CA19-9 antibody, BSA, and antigen. Differential pulse voltammetry was used to characterize this immunosensor preparation process. The assembled electrochemical immunosensor enables linear detection in the concentration range of 0.05–500 U/mL of CA19-9, and the detection limit was calculated as 0.01 U/mL. The results of the specificity measurement test showed that the signal change of the interfering substance was much lower than the response value of the detected antigen, indicating that the sensor has good specificity and strong anti-interference ability. The repeatability test results showed that the relative standard deviations were less than 5%, showing good accuracy and precision. The CA19-9 electrochemical immunosensor was used for the actual sample detection, and the experimental results of the standard serum addition method showed that the RSD values of the test concentrations were all less than 10%. The recoveries were 102.4–115.0%, indicating that the assay has high precision, good accuracy, and high potential application value.
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spelling pubmed-93242642022-07-27 Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer Wei, Zheng Cai, Xiaoping Cui, Weifeng Zhang, Junping Molecules Article A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrodes were placed in a 3-mercaptopropionic acid (MPA)/β-mercaptoethanol (ME) mixture for 24 h for self-assembly. The electrodes were then placed in an EDC/NHS mixture for 1 h. Layer modification was performed by stepwise dropwise addition of CA19-9 antibody, BSA, and antigen. Differential pulse voltammetry was used to characterize this immunosensor preparation process. The assembled electrochemical immunosensor enables linear detection in the concentration range of 0.05–500 U/mL of CA19-9, and the detection limit was calculated as 0.01 U/mL. The results of the specificity measurement test showed that the signal change of the interfering substance was much lower than the response value of the detected antigen, indicating that the sensor has good specificity and strong anti-interference ability. The repeatability test results showed that the relative standard deviations were less than 5%, showing good accuracy and precision. The CA19-9 electrochemical immunosensor was used for the actual sample detection, and the experimental results of the standard serum addition method showed that the RSD values of the test concentrations were all less than 10%. The recoveries were 102.4–115.0%, indicating that the assay has high precision, good accuracy, and high potential application value. MDPI 2022-07-18 /pmc/articles/PMC9324264/ /pubmed/35889454 http://dx.doi.org/10.3390/molecules27144578 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Zheng
Cai, Xiaoping
Cui, Weifeng
Zhang, Junping
Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer
title Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer
title_full Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer
title_fullStr Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer
title_full_unstemmed Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer
title_short Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer
title_sort electrochemical immunoassay for tumor marker ca19-9 detection based on self-assembled monolayer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324264/
https://www.ncbi.nlm.nih.gov/pubmed/35889454
http://dx.doi.org/10.3390/molecules27144578
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AT caixiaoping electrochemicalimmunoassayfortumormarkerca199detectionbasedonselfassembledmonolayer
AT cuiweifeng electrochemicalimmunoassayfortumormarkerca199detectionbasedonselfassembledmonolayer
AT zhangjunping electrochemicalimmunoassayfortumormarkerca199detectionbasedonselfassembledmonolayer