Cargando…
Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4
The inorganic selenium is absorbed and utilized inefficiently, and the range between toxicity and demand is narrow, so the application is strictly limited. Selenium nanoparticles have higher bioactivity and biosafety properties, including increased antioxidant and anticancer properties. Thus, produc...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324468/ https://www.ncbi.nlm.nih.gov/pubmed/35889450 http://dx.doi.org/10.3390/molecules27144585 |
_version_ | 1784756814391279616 |
---|---|
author | Wang, Yidan Yu, Yonghe Duan, Yuhua Wang, Qin Cong, Xin He, Yi Gao, Chao Hafeez, Muhammad Jan, Saad Rasheed, Syed Majid Cheng, Shuiyuan Wang, Zhangqian |
author_facet | Wang, Yidan Yu, Yonghe Duan, Yuhua Wang, Qin Cong, Xin He, Yi Gao, Chao Hafeez, Muhammad Jan, Saad Rasheed, Syed Majid Cheng, Shuiyuan Wang, Zhangqian |
author_sort | Wang, Yidan |
collection | PubMed |
description | The inorganic selenium is absorbed and utilized inefficiently, and the range between toxicity and demand is narrow, so the application is strictly limited. Selenium nanoparticles have higher bioactivity and biosafety properties, including increased antioxidant and anticancer properties. Thus, producing and applying eco-friendly, non-toxic selenium nanoparticles in feed additives is crucial. Bacillus paralicheniformis Y4 was investigated for its potential ability to produce selenium nanoparticles and the activity of carboxymethyl cellulases. The selenium nanoparticles were characterized using zeta potential analyses, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Additionally, evaluations of the anti-α-glucosidase activity and the antioxidant activity of the selenium nanoparticles and the ethyl acetate extracts of Y4 were conducted. B. paralicheniformis Y4 exhibited high selenite tolerance of 400 mM and the selenium nanoparticles had an average particle size of 80 nm with a zeta potential value of −35.8 mV at a pH of 7.0, suggesting that the particles are relatively stable against aggregation. After 72 h of incubation with 5 mM selenite, B. paralicheniformis Y4 was able to reduce it by 76.4%, yielding red spherical bio-derived selenium nanoparticles and increasing the carboxymethyl cellulase activity by 1.49 times to 8.96 U/mL. For the first time, this study reports that the carboxymethyl cellulase activity of Bacillus paralicheniforis was greatly enhanced by selenite. The results also indicated that B. paralicheniformis Y4 could be capable of ecologically removing selenite from contaminated sites and has great potential for producing selenium nanoparticles as feed additives to enhance the added value of agricultural products. |
format | Online Article Text |
id | pubmed-9324468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93244682022-07-27 Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 Wang, Yidan Yu, Yonghe Duan, Yuhua Wang, Qin Cong, Xin He, Yi Gao, Chao Hafeez, Muhammad Jan, Saad Rasheed, Syed Majid Cheng, Shuiyuan Wang, Zhangqian Molecules Article The inorganic selenium is absorbed and utilized inefficiently, and the range between toxicity and demand is narrow, so the application is strictly limited. Selenium nanoparticles have higher bioactivity and biosafety properties, including increased antioxidant and anticancer properties. Thus, producing and applying eco-friendly, non-toxic selenium nanoparticles in feed additives is crucial. Bacillus paralicheniformis Y4 was investigated for its potential ability to produce selenium nanoparticles and the activity of carboxymethyl cellulases. The selenium nanoparticles were characterized using zeta potential analyses, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Additionally, evaluations of the anti-α-glucosidase activity and the antioxidant activity of the selenium nanoparticles and the ethyl acetate extracts of Y4 were conducted. B. paralicheniformis Y4 exhibited high selenite tolerance of 400 mM and the selenium nanoparticles had an average particle size of 80 nm with a zeta potential value of −35.8 mV at a pH of 7.0, suggesting that the particles are relatively stable against aggregation. After 72 h of incubation with 5 mM selenite, B. paralicheniformis Y4 was able to reduce it by 76.4%, yielding red spherical bio-derived selenium nanoparticles and increasing the carboxymethyl cellulase activity by 1.49 times to 8.96 U/mL. For the first time, this study reports that the carboxymethyl cellulase activity of Bacillus paralicheniforis was greatly enhanced by selenite. The results also indicated that B. paralicheniformis Y4 could be capable of ecologically removing selenite from contaminated sites and has great potential for producing selenium nanoparticles as feed additives to enhance the added value of agricultural products. MDPI 2022-07-18 /pmc/articles/PMC9324468/ /pubmed/35889450 http://dx.doi.org/10.3390/molecules27144585 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Yidan Yu, Yonghe Duan, Yuhua Wang, Qin Cong, Xin He, Yi Gao, Chao Hafeez, Muhammad Jan, Saad Rasheed, Syed Majid Cheng, Shuiyuan Wang, Zhangqian Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 |
title | Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 |
title_full | Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 |
title_fullStr | Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 |
title_full_unstemmed | Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 |
title_short | Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4 |
title_sort | enhancing the activity of carboxymethyl cellulase enzyme using highly stable selenium nanoparticles biosynthesized by bacillus paralicheniformis y4 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324468/ https://www.ncbi.nlm.nih.gov/pubmed/35889450 http://dx.doi.org/10.3390/molecules27144585 |
work_keys_str_mv | AT wangyidan enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT yuyonghe enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT duanyuhua enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT wangqin enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT congxin enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT heyi enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT gaochao enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT hafeezmuhammad enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT jansaad enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT rasheedsyedmajid enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT chengshuiyuan enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 AT wangzhangqian enhancingtheactivityofcarboxymethylcellulaseenzymeusinghighlystableseleniumnanoparticlesbiosynthesizedbybacillusparalicheniformisy4 |