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Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat
The wheat semi-dwarfing genes Rht (Reduced height) are widely distributed among the contemporary wheat varieties. These genes also exert pleiotropic effects on plant tolerance towards various abiotic stressors. In this work, frost tolerance was studied in three near-isogenic lines of the facultative...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324540/ https://www.ncbi.nlm.nih.gov/pubmed/35887316 http://dx.doi.org/10.3390/ijms23147969 |
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author | Szalai, Gabriella Dernovics, Mihály Gondor, Orsolya Kinga Tajti, Judit Molnár, Anna Borbála Lejmel, Magdalena Anna Misheva, Svetlana Kovács, Viktória Pál, Magda Janda, Tibor |
author_facet | Szalai, Gabriella Dernovics, Mihály Gondor, Orsolya Kinga Tajti, Judit Molnár, Anna Borbála Lejmel, Magdalena Anna Misheva, Svetlana Kovács, Viktória Pál, Magda Janda, Tibor |
author_sort | Szalai, Gabriella |
collection | PubMed |
description | The wheat semi-dwarfing genes Rht (Reduced height) are widely distributed among the contemporary wheat varieties. These genes also exert pleiotropic effects on plant tolerance towards various abiotic stressors. In this work, frost tolerance was studied in three near-isogenic lines of the facultative variety ‘April Bearded’ (AB), carrying the wild type allele Rht-B1a (tall phenotype), and the mutant alleles Rht-B1b (semi-dwarf) and Rht-B1c (dwarf), and was further compared with the tolerance of a typical winter type variety, ‘Mv Beres’. The level of freezing tolerance was decreasing in the order ‘Mv Beres’ > AB Rht-B1a > AB Rht-B1b > AB Rht-B1c. To explain the observed differences, cold acclimation-related processes were studied: the expression of six cold-related genes, the phenylpropanoid pathway, carbohydrates, amino acids, polyamines and compounds in the tricarboxylic acid cycle. To achieve this, a comprehensive approach was applied, involving targeted analyses and untargeted metabolomics screening with the help of gas chromatography/liquid chromatography—mass spectrometry setups. Several cold-related processes exhibited similar changes in these genotypes; indeed, the accumulation of eight putrescine and agmatine derivatives, 17 flavones and numerous oligosaccharides (max. degree of polymerization 18) was associated with the level of freezing tolerance in the ‘April Bearded’ lines. In summary, the mutant Rht alleles may further decrease the generally low frost tolerance of the Rht-B1a, and, based on the metabolomics study, the mechanisms of frost tolerance may differ for a typical winter variety and a facultative variety. Present results point to the complex nature of frost resistance. |
format | Online Article Text |
id | pubmed-9324540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93245402022-07-27 Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat Szalai, Gabriella Dernovics, Mihály Gondor, Orsolya Kinga Tajti, Judit Molnár, Anna Borbála Lejmel, Magdalena Anna Misheva, Svetlana Kovács, Viktória Pál, Magda Janda, Tibor Int J Mol Sci Article The wheat semi-dwarfing genes Rht (Reduced height) are widely distributed among the contemporary wheat varieties. These genes also exert pleiotropic effects on plant tolerance towards various abiotic stressors. In this work, frost tolerance was studied in three near-isogenic lines of the facultative variety ‘April Bearded’ (AB), carrying the wild type allele Rht-B1a (tall phenotype), and the mutant alleles Rht-B1b (semi-dwarf) and Rht-B1c (dwarf), and was further compared with the tolerance of a typical winter type variety, ‘Mv Beres’. The level of freezing tolerance was decreasing in the order ‘Mv Beres’ > AB Rht-B1a > AB Rht-B1b > AB Rht-B1c. To explain the observed differences, cold acclimation-related processes were studied: the expression of six cold-related genes, the phenylpropanoid pathway, carbohydrates, amino acids, polyamines and compounds in the tricarboxylic acid cycle. To achieve this, a comprehensive approach was applied, involving targeted analyses and untargeted metabolomics screening with the help of gas chromatography/liquid chromatography—mass spectrometry setups. Several cold-related processes exhibited similar changes in these genotypes; indeed, the accumulation of eight putrescine and agmatine derivatives, 17 flavones and numerous oligosaccharides (max. degree of polymerization 18) was associated with the level of freezing tolerance in the ‘April Bearded’ lines. In summary, the mutant Rht alleles may further decrease the generally low frost tolerance of the Rht-B1a, and, based on the metabolomics study, the mechanisms of frost tolerance may differ for a typical winter variety and a facultative variety. Present results point to the complex nature of frost resistance. MDPI 2022-07-19 /pmc/articles/PMC9324540/ /pubmed/35887316 http://dx.doi.org/10.3390/ijms23147969 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Szalai, Gabriella Dernovics, Mihály Gondor, Orsolya Kinga Tajti, Judit Molnár, Anna Borbála Lejmel, Magdalena Anna Misheva, Svetlana Kovács, Viktória Pál, Magda Janda, Tibor Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat |
title | Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat |
title_full | Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat |
title_fullStr | Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat |
title_full_unstemmed | Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat |
title_short | Mutations in Rht-B1 Locus May Negatively Affect Frost Tolerance in Bread Wheat |
title_sort | mutations in rht-b1 locus may negatively affect frost tolerance in bread wheat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324540/ https://www.ncbi.nlm.nih.gov/pubmed/35887316 http://dx.doi.org/10.3390/ijms23147969 |
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