Lycopene Reduces the In Vitro Aging Phenotypes of Mouse Oocytes by Improving Their Oxidative Status

SIMPLE SUMMARY: Ovulation is the process of oocyte release from the ruptured mature ovarian follicle into the oviduct. Fertilization usually occurs within 10 h post-ovulation in most mammals. If fertilization is delayed, the oocyte viability and quality will decrease, with many deteriorative changes in oocyte phenotype due to oxidative stress. This process is termed postovulatory aging. Postovulatory aging is a major problem that limits the success of many assisted reproductive technologies. Lycopene is a red carotenoid dye found within tomatoes and other fruits and vegetables. Lycopene has been reported to have a strong free-radical scavenging ability. our data showed beneficial effects of lycopene supplementation of in vitro maturation media during in vitro aging of mouse oocytes by reducing the oxidative stress damages that led to their apoptosis. The present study introduces lycopene as a natural supplement to reduce the postovulatory aging of mammalian oocytes. ABSTRACT: Postovulatory aging is a major problem that limits the success of many assisted reproductive technologies (ARTs). Oxidative stress is a leading cause of oocyte aging. This study investigated the effects of lycopene supplementation of in vitro maturation (IVM) medium during the aging of mouse oocytes on the oocytes’ morphology and oxidative stress status. Mouse cumulus-oocyte complexes (COCs) were collected and cultured in the IVM medium either for 17 h, (freshly matured oocytes), or for 48 h, (in vitro-aged oocytes), with or without lycopene. The rate of fragmented and degenerated oocytes and the oocyte levels of hydrogen peroxide (H(2)O(2)), malondialdehyde (MDA), total antioxidant capacity (TAC), reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) were estimated and compared. Oocytes aged with 200 nM lycopene revealed significantly less fragmentation and degeneration, lower H(2)O(2) and MDA levels, and higher TAC, GSH and SOD levels than those aged without lycopene. CAT levels were unchanged by lycopene treatment. Taken together, our data showed beneficial effects of lycopene during in vitro aging of mouse oocytes by reducing the oxidative stress damages that lead to their apoptosis. The present study introduces lycopene as a natural supplement to reduce the postovulatory aging-dependent abnormalities of mammalian oocytes.