Cargando…
Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation
Vasopressin type-2 receptor (V2R) is ectopically expressed and plays a pathogenic role in clear cell renal cell carcinoma (ccRCC) tumor cells. Here we examined how V2R signaling within human ccRCC tumor cells (Caki1 cells) stimulates stromal cancer-associated fibroblasts (CAFs). We found that cell c...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325308/ https://www.ncbi.nlm.nih.gov/pubmed/35886951 http://dx.doi.org/10.3390/ijms23147601 |
_version_ | 1784757018097090560 |
---|---|
author | Jamadar, Abeda Dwivedi, Nidhi Mathew, Sijo Calvet, James P. Thomas, Sufi M. Rao, Reena |
author_facet | Jamadar, Abeda Dwivedi, Nidhi Mathew, Sijo Calvet, James P. Thomas, Sufi M. Rao, Reena |
author_sort | Jamadar, Abeda |
collection | PubMed |
description | Vasopressin type-2 receptor (V2R) is ectopically expressed and plays a pathogenic role in clear cell renal cell carcinoma (ccRCC) tumor cells. Here we examined how V2R signaling within human ccRCC tumor cells (Caki1 cells) stimulates stromal cancer-associated fibroblasts (CAFs). We found that cell culture conditioned media from Caki1 cells increased activation, migration, and proliferation of fibroblasts in vitro, which was inhibited by V2R gene silencing in Caki1 cells. Analysis of the conditioned media and mRNA of the V2R gene silenced and control Caki1 cells showed that V2R regulates the production of CAF-activating factors. Some of these factors were also found to be regulated by YAP in these Caki1 cells. YAP expression colocalized and correlated with V2R expression in ccRCC tumor tissue. V2R gene silencing or V2R antagonist significantly reduced YAP in Caki1 cells. Moreover, the V2R antagonist reduced YAP expression and myofibroblasts in mouse xenograft tumors. These results suggest that V2R plays an important role in secreting pro-fibrotic factors that stimulate fibroblast activation by a YAP-dependent mechanism in ccRCC tumors. Our results demonstrate a novel role for the V2R-YAP axis in the regulation of myofibroblasts in ccRCC and a potential therapeutic target. |
format | Online Article Text |
id | pubmed-9325308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93253082022-07-27 Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation Jamadar, Abeda Dwivedi, Nidhi Mathew, Sijo Calvet, James P. Thomas, Sufi M. Rao, Reena Int J Mol Sci Article Vasopressin type-2 receptor (V2R) is ectopically expressed and plays a pathogenic role in clear cell renal cell carcinoma (ccRCC) tumor cells. Here we examined how V2R signaling within human ccRCC tumor cells (Caki1 cells) stimulates stromal cancer-associated fibroblasts (CAFs). We found that cell culture conditioned media from Caki1 cells increased activation, migration, and proliferation of fibroblasts in vitro, which was inhibited by V2R gene silencing in Caki1 cells. Analysis of the conditioned media and mRNA of the V2R gene silenced and control Caki1 cells showed that V2R regulates the production of CAF-activating factors. Some of these factors were also found to be regulated by YAP in these Caki1 cells. YAP expression colocalized and correlated with V2R expression in ccRCC tumor tissue. V2R gene silencing or V2R antagonist significantly reduced YAP in Caki1 cells. Moreover, the V2R antagonist reduced YAP expression and myofibroblasts in mouse xenograft tumors. These results suggest that V2R plays an important role in secreting pro-fibrotic factors that stimulate fibroblast activation by a YAP-dependent mechanism in ccRCC tumors. Our results demonstrate a novel role for the V2R-YAP axis in the regulation of myofibroblasts in ccRCC and a potential therapeutic target. MDPI 2022-07-09 /pmc/articles/PMC9325308/ /pubmed/35886951 http://dx.doi.org/10.3390/ijms23147601 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jamadar, Abeda Dwivedi, Nidhi Mathew, Sijo Calvet, James P. Thomas, Sufi M. Rao, Reena Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation |
title | Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation |
title_full | Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation |
title_fullStr | Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation |
title_full_unstemmed | Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation |
title_short | Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation |
title_sort | vasopressin receptor type-2 mediated signaling in renal cell carcinoma stimulates stromal fibroblast activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325308/ https://www.ncbi.nlm.nih.gov/pubmed/35886951 http://dx.doi.org/10.3390/ijms23147601 |
work_keys_str_mv | AT jamadarabeda vasopressinreceptortype2mediatedsignalinginrenalcellcarcinomastimulatesstromalfibroblastactivation AT dwivedinidhi vasopressinreceptortype2mediatedsignalinginrenalcellcarcinomastimulatesstromalfibroblastactivation AT mathewsijo vasopressinreceptortype2mediatedsignalinginrenalcellcarcinomastimulatesstromalfibroblastactivation AT calvetjamesp vasopressinreceptortype2mediatedsignalinginrenalcellcarcinomastimulatesstromalfibroblastactivation AT thomassufim vasopressinreceptortype2mediatedsignalinginrenalcellcarcinomastimulatesstromalfibroblastactivation AT raoreena vasopressinreceptortype2mediatedsignalinginrenalcellcarcinomastimulatesstromalfibroblastactivation |