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A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection

SARS-CoV-2 is a novel coronavirus that has caused a global pandemic. To date, 504,907,616 people have been infected and developed coronavirus disease 2019 (COVID-19). A rapid and simple diagnostic method is needed to control this pandemic. In this study, a visual nucleic acid detection method combin...

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Autores principales: Yu, Mengtao, Huang, Pei, Li, Yuanguo, Song, Yumeng, Liu, Xingqi, Feng, Na, Jin, Hongli, Bai, Yujie, Zhang, Haili, Li, Yuanyuan, Xia, Xianzhu, Gao, Yuwei, Wang, Hualei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325585/
https://www.ncbi.nlm.nih.gov/pubmed/35903482
http://dx.doi.org/10.3389/fmicb.2022.932698
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author Yu, Mengtao
Huang, Pei
Li, Yuanguo
Song, Yumeng
Liu, Xingqi
Feng, Na
Jin, Hongli
Bai, Yujie
Zhang, Haili
Li, Yuanyuan
Xia, Xianzhu
Gao, Yuwei
Wang, Hualei
author_facet Yu, Mengtao
Huang, Pei
Li, Yuanguo
Song, Yumeng
Liu, Xingqi
Feng, Na
Jin, Hongli
Bai, Yujie
Zhang, Haili
Li, Yuanyuan
Xia, Xianzhu
Gao, Yuwei
Wang, Hualei
author_sort Yu, Mengtao
collection PubMed
description SARS-CoV-2 is a novel coronavirus that has caused a global pandemic. To date, 504,907,616 people have been infected and developed coronavirus disease 2019 (COVID-19). A rapid and simple diagnostic method is needed to control this pandemic. In this study, a visual nucleic acid detection method combining reverse transcription loop-mediated isothermal amplification and a vertical flow visualization strip (RT-LAMP-VF) was successfully established and could detect 20 copies/μl of SARS-CoV-2 RNA transcript within 50 min at 61°C. This assay had no cross-reactivity with a variety of coronaviruses, including human coronavirus OC43, 229E, HKU1, NL63, severe acute respiratory syndrome-related coronavirus (SARSr-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and bat coronavirus HKU4, exhibiting very high levels of diagnostic sensitivity and specificity. Most strikingly, this method can be used for detecting multiple SARS-CoV-2 variants, including the Wuhan-Hu-1 strain, Delta, and Omicron variants. Compared with the RT-qPCR method recommended by the World Health Organization (WHO), RT-LAMP-VF does not require special equipment and is easy to perform. As a result, it is more suitable for rapid screening of suspected SARS-CoV-2 samples in the field and local laboratories.
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spelling pubmed-93255852022-07-27 A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection Yu, Mengtao Huang, Pei Li, Yuanguo Song, Yumeng Liu, Xingqi Feng, Na Jin, Hongli Bai, Yujie Zhang, Haili Li, Yuanyuan Xia, Xianzhu Gao, Yuwei Wang, Hualei Front Microbiol Microbiology SARS-CoV-2 is a novel coronavirus that has caused a global pandemic. To date, 504,907,616 people have been infected and developed coronavirus disease 2019 (COVID-19). A rapid and simple diagnostic method is needed to control this pandemic. In this study, a visual nucleic acid detection method combining reverse transcription loop-mediated isothermal amplification and a vertical flow visualization strip (RT-LAMP-VF) was successfully established and could detect 20 copies/μl of SARS-CoV-2 RNA transcript within 50 min at 61°C. This assay had no cross-reactivity with a variety of coronaviruses, including human coronavirus OC43, 229E, HKU1, NL63, severe acute respiratory syndrome-related coronavirus (SARSr-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and bat coronavirus HKU4, exhibiting very high levels of diagnostic sensitivity and specificity. Most strikingly, this method can be used for detecting multiple SARS-CoV-2 variants, including the Wuhan-Hu-1 strain, Delta, and Omicron variants. Compared with the RT-qPCR method recommended by the World Health Organization (WHO), RT-LAMP-VF does not require special equipment and is easy to perform. As a result, it is more suitable for rapid screening of suspected SARS-CoV-2 samples in the field and local laboratories. Frontiers Media S.A. 2022-07-13 /pmc/articles/PMC9325585/ /pubmed/35903482 http://dx.doi.org/10.3389/fmicb.2022.932698 Text en Copyright © 2022 Yu, Huang, Li, Song, Liu, Feng, Jin, Bai, Zhang, Li, Xia, Gao and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yu, Mengtao
Huang, Pei
Li, Yuanguo
Song, Yumeng
Liu, Xingqi
Feng, Na
Jin, Hongli
Bai, Yujie
Zhang, Haili
Li, Yuanyuan
Xia, Xianzhu
Gao, Yuwei
Wang, Hualei
A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection
title A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection
title_full A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection
title_fullStr A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection
title_full_unstemmed A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection
title_short A Visual Assay of a Loop-Mediated Isothermal Amplification Based Vertical Immunoassay for SARS-CoV-2 RNA Detection
title_sort visual assay of a loop-mediated isothermal amplification based vertical immunoassay for sars-cov-2 rna detection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325585/
https://www.ncbi.nlm.nih.gov/pubmed/35903482
http://dx.doi.org/10.3389/fmicb.2022.932698
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