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The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin

Exposure to antibiotics most often generates oxidative stress in bacteria. Oxidative stress survival mechanisms would facilitate the evolution of antibiotic resistance. As part of an effort to understand oxidative stress survival mechanisms in mycobacteria, here we show that the minor subpopulation...

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Autores principales: Nair, Rashmi Ravindran, Sharan, Deepti, Srinivasan, Vijay, Mukkayyan, Nagaraja, Jakkala, Kishor, Ajitkumar, Parthasarathi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325904/
https://www.ncbi.nlm.nih.gov/pubmed/35909613
http://dx.doi.org/10.1016/j.crmicr.2022.100148
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author Nair, Rashmi Ravindran
Sharan, Deepti
Srinivasan, Vijay
Mukkayyan, Nagaraja
Jakkala, Kishor
Ajitkumar, Parthasarathi
author_facet Nair, Rashmi Ravindran
Sharan, Deepti
Srinivasan, Vijay
Mukkayyan, Nagaraja
Jakkala, Kishor
Ajitkumar, Parthasarathi
author_sort Nair, Rashmi Ravindran
collection PubMed
description Exposure to antibiotics most often generates oxidative stress in bacteria. Oxidative stress survival mechanisms would facilitate the evolution of antibiotic resistance. As part of an effort to understand oxidative stress survival mechanisms in mycobacteria, here we show that the minor subpopulation (SCs; short-sized cells constituting 10% of the population) of Mycobacterium smegmatis significantly increased the survival of its major kin subpopulation (NCs; normal/long-sized cells constituting 90% of the population) in the mid-log-phase (MLP) cultures against the oxidative stress induced by rifampicin and exogenously added H(2)O(2) (positive control). We had earlier shown that the SCs in the MLP cultures inherently and naturally release significantly high levels of H(2)O(2) into the medium. Addition of the SCs’ culture supernatant, unlike the supernatant of the dimethylthiourea (H(2)O(2) scavenger) exposed SCs, enhanced the survival of NCs. It indicated that NCs’ survival required the H(2)O(2) present in the SCs’ supernatant. This H(2)O(2) transcriptionally induced high levels of catalase-peroxidase (KatG) in the NCs. The naturally high KatG levels in the NCs significantly neutralised the endogenous H(2)O(2) formed upon exposure to rifampicin or H(2)O(2), thereby enhancing the survival of NCs against oxidative stress. The absence of such enhanced survival in the furA-katG and katG knockout (KO) mutants of NCs in the presence of wild-type SCs, confirmed the requirement of the H(2)O(2) present in the SCs’ supernatant and NCs’ KatG for enhanced oxidative stress survival. The presence of SCs:NCs at 1:9 in the pulmonary tuberculosis patients’ sputum alludes to the clinical significance of the finding.
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spelling pubmed-93259042022-07-28 The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin Nair, Rashmi Ravindran Sharan, Deepti Srinivasan, Vijay Mukkayyan, Nagaraja Jakkala, Kishor Ajitkumar, Parthasarathi Curr Res Microb Sci Research Paper Exposure to antibiotics most often generates oxidative stress in bacteria. Oxidative stress survival mechanisms would facilitate the evolution of antibiotic resistance. As part of an effort to understand oxidative stress survival mechanisms in mycobacteria, here we show that the minor subpopulation (SCs; short-sized cells constituting 10% of the population) of Mycobacterium smegmatis significantly increased the survival of its major kin subpopulation (NCs; normal/long-sized cells constituting 90% of the population) in the mid-log-phase (MLP) cultures against the oxidative stress induced by rifampicin and exogenously added H(2)O(2) (positive control). We had earlier shown that the SCs in the MLP cultures inherently and naturally release significantly high levels of H(2)O(2) into the medium. Addition of the SCs’ culture supernatant, unlike the supernatant of the dimethylthiourea (H(2)O(2) scavenger) exposed SCs, enhanced the survival of NCs. It indicated that NCs’ survival required the H(2)O(2) present in the SCs’ supernatant. This H(2)O(2) transcriptionally induced high levels of catalase-peroxidase (KatG) in the NCs. The naturally high KatG levels in the NCs significantly neutralised the endogenous H(2)O(2) formed upon exposure to rifampicin or H(2)O(2), thereby enhancing the survival of NCs against oxidative stress. The absence of such enhanced survival in the furA-katG and katG knockout (KO) mutants of NCs in the presence of wild-type SCs, confirmed the requirement of the H(2)O(2) present in the SCs’ supernatant and NCs’ KatG for enhanced oxidative stress survival. The presence of SCs:NCs at 1:9 in the pulmonary tuberculosis patients’ sputum alludes to the clinical significance of the finding. Elsevier 2022-06-18 /pmc/articles/PMC9325904/ /pubmed/35909613 http://dx.doi.org/10.1016/j.crmicr.2022.100148 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Nair, Rashmi Ravindran
Sharan, Deepti
Srinivasan, Vijay
Mukkayyan, Nagaraja
Jakkala, Kishor
Ajitkumar, Parthasarathi
The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
title The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
title_full The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
title_fullStr The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
title_full_unstemmed The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
title_short The H(2)O(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
title_sort h(2)o(2) inherently released by the mycobacterial minor subpopulation enhances the survival of the major kin subpopulation against rifampicin
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325904/
https://www.ncbi.nlm.nih.gov/pubmed/35909613
http://dx.doi.org/10.1016/j.crmicr.2022.100148
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