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Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México

This is the first report of the genetic diversity of the Mycobacterium tuberculosis complex isolates found in a Mexican-Amerindian setting. In this study, we analyzed isolates collected from the Highlands region of Chiapas, Mexico, by using spoligotyping and whole-genome sequencing analyses. Seventy...

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Autores principales: Molina-Torres, Carmen A., Quinn, Frederick D., Castro-Garza, Jorge, Gómez-Velasco, Anaximandro, Ocampo-Candiani, Jorge, Bencomo-Alerm, Alied, Sánchez-Pérez, Héctor Javier, Muñoz-Jiménez, Sergio, Rendón, Adrián, Ansari, Afzal, Sharma, Mukul, Singh, Pushpendra, Vera-Cabrera, Lucio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326120/
https://www.ncbi.nlm.nih.gov/pubmed/35909960
http://dx.doi.org/10.3389/fcimb.2022.875909
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author Molina-Torres, Carmen A.
Quinn, Frederick D.
Castro-Garza, Jorge
Gómez-Velasco, Anaximandro
Ocampo-Candiani, Jorge
Bencomo-Alerm, Alied
Sánchez-Pérez, Héctor Javier
Muñoz-Jiménez, Sergio
Rendón, Adrián
Ansari, Afzal
Sharma, Mukul
Singh, Pushpendra
Vera-Cabrera, Lucio
author_facet Molina-Torres, Carmen A.
Quinn, Frederick D.
Castro-Garza, Jorge
Gómez-Velasco, Anaximandro
Ocampo-Candiani, Jorge
Bencomo-Alerm, Alied
Sánchez-Pérez, Héctor Javier
Muñoz-Jiménez, Sergio
Rendón, Adrián
Ansari, Afzal
Sharma, Mukul
Singh, Pushpendra
Vera-Cabrera, Lucio
author_sort Molina-Torres, Carmen A.
collection PubMed
description This is the first report of the genetic diversity of the Mycobacterium tuberculosis complex isolates found in a Mexican-Amerindian setting. In this study, we analyzed isolates collected from the Highlands region of Chiapas, Mexico, by using spoligotyping and whole-genome sequencing analyses. Seventy-three M. tuberculosis isolates were analyzed initially by spoligotyping; no new spoligotypes were identified. Nineteen percent of the isolates were identified as SIT53 (T1) (n = 14), followed by SIT42 (14%, n = 10, LAM9) and SIT119 (11%; n = 8, X1). SIT53, SIT42, and orphan isolates (16.4%, n = 12) constituted about 50% of the isolates studied and were subjected to whole-genome sequencing (WGS) analysis. Most SIT53 (10/12) isolates belonged to the Euro-American sub-lineage 4.8. Most SIT42 isolates (4/7) as .well as most orphan isolates (5/8) belonged to the lineage 4.3.3 LAM group. By comparing the single-nucleotide polymorphism (SNP) patterns of the SIT53 isolates, we found one clone (<7 SNPs) and four clustered isolates (<15 SNPs). In isolates from the SIT42 and orphan groups, we did not find any clones or clusters. This work demonstrates the success of sub-lineage 4.8 to predominate in Mexico and confirms the dominion of sub-lineage 4.3.3 in Central and South America.
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spelling pubmed-93261202022-07-28 Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México Molina-Torres, Carmen A. Quinn, Frederick D. Castro-Garza, Jorge Gómez-Velasco, Anaximandro Ocampo-Candiani, Jorge Bencomo-Alerm, Alied Sánchez-Pérez, Héctor Javier Muñoz-Jiménez, Sergio Rendón, Adrián Ansari, Afzal Sharma, Mukul Singh, Pushpendra Vera-Cabrera, Lucio Front Cell Infect Microbiol Cellular and Infection Microbiology This is the first report of the genetic diversity of the Mycobacterium tuberculosis complex isolates found in a Mexican-Amerindian setting. In this study, we analyzed isolates collected from the Highlands region of Chiapas, Mexico, by using spoligotyping and whole-genome sequencing analyses. Seventy-three M. tuberculosis isolates were analyzed initially by spoligotyping; no new spoligotypes were identified. Nineteen percent of the isolates were identified as SIT53 (T1) (n = 14), followed by SIT42 (14%, n = 10, LAM9) and SIT119 (11%; n = 8, X1). SIT53, SIT42, and orphan isolates (16.4%, n = 12) constituted about 50% of the isolates studied and were subjected to whole-genome sequencing (WGS) analysis. Most SIT53 (10/12) isolates belonged to the Euro-American sub-lineage 4.8. Most SIT42 isolates (4/7) as .well as most orphan isolates (5/8) belonged to the lineage 4.3.3 LAM group. By comparing the single-nucleotide polymorphism (SNP) patterns of the SIT53 isolates, we found one clone (<7 SNPs) and four clustered isolates (<15 SNPs). In isolates from the SIT42 and orphan groups, we did not find any clones or clusters. This work demonstrates the success of sub-lineage 4.8 to predominate in Mexico and confirms the dominion of sub-lineage 4.3.3 in Central and South America. Frontiers Media S.A. 2022-07-13 /pmc/articles/PMC9326120/ /pubmed/35909960 http://dx.doi.org/10.3389/fcimb.2022.875909 Text en Copyright © 2022 Molina-Torres, Quinn, Castro-Garza, Gómez-Velasco, Ocampo-Candiani, Bencomo-Alerm, Sánchez-Pérez, Muñoz-Jiménez, Rendón, Ansari, Sharma, Singh and Vera-Cabrera https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Molina-Torres, Carmen A.
Quinn, Frederick D.
Castro-Garza, Jorge
Gómez-Velasco, Anaximandro
Ocampo-Candiani, Jorge
Bencomo-Alerm, Alied
Sánchez-Pérez, Héctor Javier
Muñoz-Jiménez, Sergio
Rendón, Adrián
Ansari, Afzal
Sharma, Mukul
Singh, Pushpendra
Vera-Cabrera, Lucio
Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México
title Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México
title_full Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México
title_fullStr Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México
title_full_unstemmed Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México
title_short Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México
title_sort genetic diversity of mycobacterium tuberculosis isolates from an amerindian population in chiapas, méxico
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326120/
https://www.ncbi.nlm.nih.gov/pubmed/35909960
http://dx.doi.org/10.3389/fcimb.2022.875909
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