Cargando…
Mapping molecular complexes with super-resolution microscopy and single-particle analysis
Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular s...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326279/ https://www.ncbi.nlm.nih.gov/pubmed/35892200 http://dx.doi.org/10.1098/rsob.220079 |
_version_ | 1784757248103284736 |
---|---|
author | Mendes, Afonso Heil, Hannah S. Coelho, Simao Leterrier, Christophe Henriques, Ricardo |
author_facet | Mendes, Afonso Heil, Hannah S. Coelho, Simao Leterrier, Christophe Henriques, Ricardo |
author_sort | Mendes, Afonso |
collection | PubMed |
description | Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM–SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail. |
format | Online Article Text |
id | pubmed-9326279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93262792022-07-31 Mapping molecular complexes with super-resolution microscopy and single-particle analysis Mendes, Afonso Heil, Hannah S. Coelho, Simao Leterrier, Christophe Henriques, Ricardo Open Biol Special Feature Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM–SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail. The Royal Society 2022-07-27 /pmc/articles/PMC9326279/ /pubmed/35892200 http://dx.doi.org/10.1098/rsob.220079 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Special Feature Mendes, Afonso Heil, Hannah S. Coelho, Simao Leterrier, Christophe Henriques, Ricardo Mapping molecular complexes with super-resolution microscopy and single-particle analysis |
title | Mapping molecular complexes with super-resolution microscopy and single-particle analysis |
title_full | Mapping molecular complexes with super-resolution microscopy and single-particle analysis |
title_fullStr | Mapping molecular complexes with super-resolution microscopy and single-particle analysis |
title_full_unstemmed | Mapping molecular complexes with super-resolution microscopy and single-particle analysis |
title_short | Mapping molecular complexes with super-resolution microscopy and single-particle analysis |
title_sort | mapping molecular complexes with super-resolution microscopy and single-particle analysis |
topic | Special Feature |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326279/ https://www.ncbi.nlm.nih.gov/pubmed/35892200 http://dx.doi.org/10.1098/rsob.220079 |
work_keys_str_mv | AT mendesafonso mappingmolecularcomplexeswithsuperresolutionmicroscopyandsingleparticleanalysis AT heilhannahs mappingmolecularcomplexeswithsuperresolutionmicroscopyandsingleparticleanalysis AT coelhosimao mappingmolecularcomplexeswithsuperresolutionmicroscopyandsingleparticleanalysis AT leterrierchristophe mappingmolecularcomplexeswithsuperresolutionmicroscopyandsingleparticleanalysis AT henriquesricardo mappingmolecularcomplexeswithsuperresolutionmicroscopyandsingleparticleanalysis |