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Mapping molecular complexes with super-resolution microscopy and single-particle analysis

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular s...

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Detalles Bibliográficos
Autores principales: Mendes, Afonso, Heil, Hannah S., Coelho, Simao, Leterrier, Christophe, Henriques, Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326279/
https://www.ncbi.nlm.nih.gov/pubmed/35892200
http://dx.doi.org/10.1098/rsob.220079
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author Mendes, Afonso
Heil, Hannah S.
Coelho, Simao
Leterrier, Christophe
Henriques, Ricardo
author_facet Mendes, Afonso
Heil, Hannah S.
Coelho, Simao
Leterrier, Christophe
Henriques, Ricardo
author_sort Mendes, Afonso
collection PubMed
description Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM–SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.
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spelling pubmed-93262792022-07-31 Mapping molecular complexes with super-resolution microscopy and single-particle analysis Mendes, Afonso Heil, Hannah S. Coelho, Simao Leterrier, Christophe Henriques, Ricardo Open Biol Special Feature Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM–SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail. The Royal Society 2022-07-27 /pmc/articles/PMC9326279/ /pubmed/35892200 http://dx.doi.org/10.1098/rsob.220079 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Special Feature
Mendes, Afonso
Heil, Hannah S.
Coelho, Simao
Leterrier, Christophe
Henriques, Ricardo
Mapping molecular complexes with super-resolution microscopy and single-particle analysis
title Mapping molecular complexes with super-resolution microscopy and single-particle analysis
title_full Mapping molecular complexes with super-resolution microscopy and single-particle analysis
title_fullStr Mapping molecular complexes with super-resolution microscopy and single-particle analysis
title_full_unstemmed Mapping molecular complexes with super-resolution microscopy and single-particle analysis
title_short Mapping molecular complexes with super-resolution microscopy and single-particle analysis
title_sort mapping molecular complexes with super-resolution microscopy and single-particle analysis
topic Special Feature
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326279/
https://www.ncbi.nlm.nih.gov/pubmed/35892200
http://dx.doi.org/10.1098/rsob.220079
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