Cargando…

Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys

Non-invasive health monitoring is advantageous for wild and captive primate populations because it reduces the need for traditional invasive techniques (i.e., anesthetization) that can be stressful and potentially harmful for individuals. The biomarker neopterin is an emerging tool in primatology to...

Descripción completa

Detalles Bibliográficos
Autores principales: Lucore, Jordan M., Marshall, Andrew J., Brosnan, Sarah F., Benítez, Marcela E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326447/
https://www.ncbi.nlm.nih.gov/pubmed/35909690
http://dx.doi.org/10.3389/fvets.2022.918036
_version_ 1784757288002650112
author Lucore, Jordan M.
Marshall, Andrew J.
Brosnan, Sarah F.
Benítez, Marcela E.
author_facet Lucore, Jordan M.
Marshall, Andrew J.
Brosnan, Sarah F.
Benítez, Marcela E.
author_sort Lucore, Jordan M.
collection PubMed
description Non-invasive health monitoring is advantageous for wild and captive primate populations because it reduces the need for traditional invasive techniques (i.e., anesthetization) that can be stressful and potentially harmful for individuals. The biomarker neopterin is an emerging tool in primatology to measure immune activation and immunosenescence, however, most neopterin studies have focused on catarrhine species with little comparative work examining neopterin and health in platyrrhines. To address this gap, we validated a commercially available enzyme-linked immunosorbent assay (ELISA) to measure urinary neopterin in two types of capuchin monkeys, a wild population of white-faced capuchins (Cebus imitator) and a socially housed captive colony of tufted capuchins (Sapajus apella). We analytically validated methods for measuring urinary neopterin in two capuchin populations and demonstrated that two commonly-used methods to control for urine concentration—creatinine and specific gravity (SG)—produced highly concordant results. We also biologically validated these methods by examining variation in neopterin levels based on environment (captive and wild) and age, and changes in levels associated with immune-response. We found that neopterin increased after immune perturbation (rabies vaccine booster), varied by environmental condition, and mirrored expected trends in immune system ontogeny. Our results improve understanding of the innate immune system in platyrrhine species and suggest neopterin may be useful for non-invasive health monitoring in both captive and wild primates.
format Online
Article
Text
id pubmed-9326447
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93264472022-07-28 Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys Lucore, Jordan M. Marshall, Andrew J. Brosnan, Sarah F. Benítez, Marcela E. Front Vet Sci Veterinary Science Non-invasive health monitoring is advantageous for wild and captive primate populations because it reduces the need for traditional invasive techniques (i.e., anesthetization) that can be stressful and potentially harmful for individuals. The biomarker neopterin is an emerging tool in primatology to measure immune activation and immunosenescence, however, most neopterin studies have focused on catarrhine species with little comparative work examining neopterin and health in platyrrhines. To address this gap, we validated a commercially available enzyme-linked immunosorbent assay (ELISA) to measure urinary neopterin in two types of capuchin monkeys, a wild population of white-faced capuchins (Cebus imitator) and a socially housed captive colony of tufted capuchins (Sapajus apella). We analytically validated methods for measuring urinary neopterin in two capuchin populations and demonstrated that two commonly-used methods to control for urine concentration—creatinine and specific gravity (SG)—produced highly concordant results. We also biologically validated these methods by examining variation in neopterin levels based on environment (captive and wild) and age, and changes in levels associated with immune-response. We found that neopterin increased after immune perturbation (rabies vaccine booster), varied by environmental condition, and mirrored expected trends in immune system ontogeny. Our results improve understanding of the innate immune system in platyrrhine species and suggest neopterin may be useful for non-invasive health monitoring in both captive and wild primates. Frontiers Media S.A. 2022-07-13 /pmc/articles/PMC9326447/ /pubmed/35909690 http://dx.doi.org/10.3389/fvets.2022.918036 Text en Copyright © 2022 Lucore, Marshall, Brosnan and Benítez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Lucore, Jordan M.
Marshall, Andrew J.
Brosnan, Sarah F.
Benítez, Marcela E.
Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys
title Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys
title_full Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys
title_fullStr Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys
title_full_unstemmed Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys
title_short Validating Urinary Neopterin as a Biomarker of Immune Response in Captive and Wild Capuchin Monkeys
title_sort validating urinary neopterin as a biomarker of immune response in captive and wild capuchin monkeys
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326447/
https://www.ncbi.nlm.nih.gov/pubmed/35909690
http://dx.doi.org/10.3389/fvets.2022.918036
work_keys_str_mv AT lucorejordanm validatingurinaryneopterinasabiomarkerofimmuneresponseincaptiveandwildcapuchinmonkeys
AT marshallandrewj validatingurinaryneopterinasabiomarkerofimmuneresponseincaptiveandwildcapuchinmonkeys
AT brosnansarahf validatingurinaryneopterinasabiomarkerofimmuneresponseincaptiveandwildcapuchinmonkeys
AT benitezmarcelae validatingurinaryneopterinasabiomarkerofimmuneresponseincaptiveandwildcapuchinmonkeys