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Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis

Astrocytes influence sleep expression and regulation, but the cellular signaling pathways involved in these processes are poorly defined. We proposed that astrocytes detect and integrate a neuronal signal that accumulates during wakefulness, thereby leading to increased sleep drive. Noradrenaline (N...

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Autores principales: Ingiosi, Ashley M., Frank, Marcos G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326550/
https://www.ncbi.nlm.nih.gov/pubmed/35892990
http://dx.doi.org/10.3390/clockssleep4030028
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author Ingiosi, Ashley M.
Frank, Marcos G.
author_facet Ingiosi, Ashley M.
Frank, Marcos G.
author_sort Ingiosi, Ashley M.
collection PubMed
description Astrocytes influence sleep expression and regulation, but the cellular signaling pathways involved in these processes are poorly defined. We proposed that astrocytes detect and integrate a neuronal signal that accumulates during wakefulness, thereby leading to increased sleep drive. Noradrenaline (NA) satisfies several criteria for a waking signal integrated by astrocytes. We therefore investigated the role of NA signaling in astrocytes in mammalian sleep. We conditionally knocked out (cKO) β2-adrenergic receptors (β2-AR) selectively in astrocytes in mice and recorded electroencephalographic and electromyographic activity under baseline conditions and in response to sleep deprivation (SDep). cKO of astroglial β2-ARs increased active phase siesta duration under baseline conditions and reduced homeostatic compensatory changes in sleep consolidation and non-rapid eye movement slow-wave activity (SWA) after SDep. Overall, astroglial NA β2-ARs influence mammalian sleep homeostasis in a manner consistent with our proposed model of neuronal–astroglial interactions.
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spelling pubmed-93265502022-07-28 Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis Ingiosi, Ashley M. Frank, Marcos G. Clocks Sleep Article Astrocytes influence sleep expression and regulation, but the cellular signaling pathways involved in these processes are poorly defined. We proposed that astrocytes detect and integrate a neuronal signal that accumulates during wakefulness, thereby leading to increased sleep drive. Noradrenaline (NA) satisfies several criteria for a waking signal integrated by astrocytes. We therefore investigated the role of NA signaling in astrocytes in mammalian sleep. We conditionally knocked out (cKO) β2-adrenergic receptors (β2-AR) selectively in astrocytes in mice and recorded electroencephalographic and electromyographic activity under baseline conditions and in response to sleep deprivation (SDep). cKO of astroglial β2-ARs increased active phase siesta duration under baseline conditions and reduced homeostatic compensatory changes in sleep consolidation and non-rapid eye movement slow-wave activity (SWA) after SDep. Overall, astroglial NA β2-ARs influence mammalian sleep homeostasis in a manner consistent with our proposed model of neuronal–astroglial interactions. MDPI 2022-07-07 /pmc/articles/PMC9326550/ /pubmed/35892990 http://dx.doi.org/10.3390/clockssleep4030028 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ingiosi, Ashley M.
Frank, Marcos G.
Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
title Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
title_full Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
title_fullStr Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
title_full_unstemmed Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
title_short Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
title_sort noradrenergic signaling in astrocytes influences mammalian sleep homeostasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326550/
https://www.ncbi.nlm.nih.gov/pubmed/35892990
http://dx.doi.org/10.3390/clockssleep4030028
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