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Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology
Combinatorial biology methods such as phage and yeast display, suitable for the generation and screening of huge numbers of protein fragments and mutated variants, have been useful when dissecting the molecular details of the interactions between antibodies and their target antigens (mainly those of...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326607/ https://www.ncbi.nlm.nih.gov/pubmed/35892708 http://dx.doi.org/10.3390/antib11030048 |
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author | Rojas, Gertrudis |
author_facet | Rojas, Gertrudis |
author_sort | Rojas, Gertrudis |
collection | PubMed |
description | Combinatorial biology methods such as phage and yeast display, suitable for the generation and screening of huge numbers of protein fragments and mutated variants, have been useful when dissecting the molecular details of the interactions between antibodies and their target antigens (mainly those of protein nature). The relevance of these studies goes far beyond the mere description of binding interfaces, as the information obtained has implications for the understanding of the chemistry of antibody–antigen binding reactions and the biological effects of antibodies. Further modification of the interactions through combinatorial methods to manipulate the key properties of antibodies (affinity and fine specificity) can result in the emergence of novel research tools and optimized therapeutics. |
format | Online Article Text |
id | pubmed-9326607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93266072022-07-28 Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology Rojas, Gertrudis Antibodies (Basel) Review Combinatorial biology methods such as phage and yeast display, suitable for the generation and screening of huge numbers of protein fragments and mutated variants, have been useful when dissecting the molecular details of the interactions between antibodies and their target antigens (mainly those of protein nature). The relevance of these studies goes far beyond the mere description of binding interfaces, as the information obtained has implications for the understanding of the chemistry of antibody–antigen binding reactions and the biological effects of antibodies. Further modification of the interactions through combinatorial methods to manipulate the key properties of antibodies (affinity and fine specificity) can result in the emergence of novel research tools and optimized therapeutics. MDPI 2022-07-14 /pmc/articles/PMC9326607/ /pubmed/35892708 http://dx.doi.org/10.3390/antib11030048 Text en © 2022 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Rojas, Gertrudis Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology |
title | Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology |
title_full | Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology |
title_fullStr | Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology |
title_full_unstemmed | Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology |
title_short | Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology |
title_sort | understanding and modulating antibody fine specificity: lessons from combinatorial biology |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326607/ https://www.ncbi.nlm.nih.gov/pubmed/35892708 http://dx.doi.org/10.3390/antib11030048 |
work_keys_str_mv | AT rojasgertrudis understandingandmodulatingantibodyfinespecificitylessonsfromcombinatorialbiology |