Cargando…

FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway

BACKGROUND: As prevalent cancer in women, approximately 569,847 cases of cervical cancer occur every year. AIMS: This study aimed to explore the role of FLOT2 and its related mechanism in the development of cervical cancer. STUDY DESIGN: Cell culture study and animal experimentation. METHODS: Quanti...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yang, Dou, Shulan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326943/
https://www.ncbi.nlm.nih.gov/pubmed/35872627
http://dx.doi.org/10.4274/balkanmedj.galenos.2022.2022-3-109
_version_ 1784757402634027008
author Li, Yang
Dou, Shulan
author_facet Li, Yang
Dou, Shulan
author_sort Li, Yang
collection PubMed
description BACKGROUND: As prevalent cancer in women, approximately 569,847 cases of cervical cancer occur every year. AIMS: This study aimed to explore the role of FLOT2 and its related mechanism in the development of cervical cancer. STUDY DESIGN: Cell culture study and animal experimentation. METHODS: Quantitative reverse-transcription polymerase chain reaction PCR and Western blot analysis were performed to evaluate the expression of FLOT2. Flow cytometry was applied for the evaluation of cell apoptosis. Cell Counting Kit-8 and colony formation were utilized for proliferation measurement. Cervical cancer mice model was employed to measure the role of FLOT2 in vivo. RESULTS: FLOT2 mRNA and protein levels were dramatically elevated (P < 0.001) in cervical cancer cell line HcerEpic cells. The cell viability and proliferation of cervical cancer cells were enhanced (P < 0.01) by overexpression of FLOT2 and reduced (P < 0.01) by FLOT2 downregulation. In addition, FLOT2 overexpression elevated (P < 0.01) the cell migration abilities of cervical cancer cells, whereas its depletion inhibited (P < 0.01) the cell migration abilities. Moreover, the protein expression of epithelial-mesenchymal transition markers including Vimentin, N-cadherin, and E-cadherin were assessed, and the results showed enhanced Vimentin and N-cadherin levels (P < 0.05) by FLOT2 upregulation and declined (P < 0.01) by FLOT2 downregulation. FLOT2 upregulation reduced (P < 0.05) the level of E-cadherin protein, whereas FLOT2 suppression attenuated this effect (P < 0.05). Furthermore, FLOT2 increased (P < 0.05) p-MEK/MEK, p-ERK1/2/ERK1/2, and p-AKT/AKT levels to activate the MEK/ERK1/2 and AKT pathways in cervical cancer. Finally, our results indicated that FLOT2 inhibited (P < 0.001) cervical cancer growth in vivo. CONCLUSION: FLOT2 aggravates the proliferation and epithelial-mesenchymal transition of cervical cancer by activating the MEK/ERK1/2 and AKT pathways.
format Online
Article
Text
id pubmed-9326943
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Galenos Publishing
record_format MEDLINE/PubMed
spelling pubmed-93269432022-08-09 FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway Li, Yang Dou, Shulan Balkan Med J Original Article BACKGROUND: As prevalent cancer in women, approximately 569,847 cases of cervical cancer occur every year. AIMS: This study aimed to explore the role of FLOT2 and its related mechanism in the development of cervical cancer. STUDY DESIGN: Cell culture study and animal experimentation. METHODS: Quantitative reverse-transcription polymerase chain reaction PCR and Western blot analysis were performed to evaluate the expression of FLOT2. Flow cytometry was applied for the evaluation of cell apoptosis. Cell Counting Kit-8 and colony formation were utilized for proliferation measurement. Cervical cancer mice model was employed to measure the role of FLOT2 in vivo. RESULTS: FLOT2 mRNA and protein levels were dramatically elevated (P < 0.001) in cervical cancer cell line HcerEpic cells. The cell viability and proliferation of cervical cancer cells were enhanced (P < 0.01) by overexpression of FLOT2 and reduced (P < 0.01) by FLOT2 downregulation. In addition, FLOT2 overexpression elevated (P < 0.01) the cell migration abilities of cervical cancer cells, whereas its depletion inhibited (P < 0.01) the cell migration abilities. Moreover, the protein expression of epithelial-mesenchymal transition markers including Vimentin, N-cadherin, and E-cadherin were assessed, and the results showed enhanced Vimentin and N-cadherin levels (P < 0.05) by FLOT2 upregulation and declined (P < 0.01) by FLOT2 downregulation. FLOT2 upregulation reduced (P < 0.05) the level of E-cadherin protein, whereas FLOT2 suppression attenuated this effect (P < 0.05). Furthermore, FLOT2 increased (P < 0.05) p-MEK/MEK, p-ERK1/2/ERK1/2, and p-AKT/AKT levels to activate the MEK/ERK1/2 and AKT pathways in cervical cancer. Finally, our results indicated that FLOT2 inhibited (P < 0.001) cervical cancer growth in vivo. CONCLUSION: FLOT2 aggravates the proliferation and epithelial-mesenchymal transition of cervical cancer by activating the MEK/ERK1/2 and AKT pathways. Galenos Publishing 2022-07-22 /pmc/articles/PMC9326943/ /pubmed/35872627 http://dx.doi.org/10.4274/balkanmedj.galenos.2022.2022-3-109 Text en ©Copyright 2022 by Trakya University Faculty of Medicine https://creativecommons.org/licenses/by-nc-nd/4.0/The Balkan Medical Journal published by Galenos Publishing House.
spellingShingle Original Article
Li, Yang
Dou, Shulan
FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway
title FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway
title_full FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway
title_fullStr FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway
title_full_unstemmed FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway
title_short FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway
title_sort flot2 promotes the proliferation and epithelial-mesenchymal transition of cervical cancer by activating the mek/erk1/2 pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326943/
https://www.ncbi.nlm.nih.gov/pubmed/35872627
http://dx.doi.org/10.4274/balkanmedj.galenos.2022.2022-3-109
work_keys_str_mv AT liyang flot2promotestheproliferationandepithelialmesenchymaltransitionofcervicalcancerbyactivatingthemekerk12pathway
AT doushulan flot2promotestheproliferationandepithelialmesenchymaltransitionofcervicalcancerbyactivatingthemekerk12pathway