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Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells
BACKGROUND: Periodontitis can lead to progressive destruction of periodontal tissues supporting the tooth. Developing biomaterials for tissue engineering has noticeably improved the existing treatment options. The present study investigated the gelatin-hydroxyapatite nano-fibers as promising scaffol...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327463/ https://www.ncbi.nlm.nih.gov/pubmed/35919304 http://dx.doi.org/10.34172/japid.2020.001 |
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author | Sharifi, Simin Samiei, Mohammad Dalir Abdolahinia, Elaheh Khalilov, Rovshan Shahi, Shahriar Maleki Dizaj, Solmaz |
author_facet | Sharifi, Simin Samiei, Mohammad Dalir Abdolahinia, Elaheh Khalilov, Rovshan Shahi, Shahriar Maleki Dizaj, Solmaz |
author_sort | Sharifi, Simin |
collection | PubMed |
description | BACKGROUND: Periodontitis can lead to progressive destruction of periodontal tissues supporting the tooth. Developing biomaterials for tissue engineering has noticeably improved the existing treatment options. The present study investigated the gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR). METHODS: The scaffolds were prepared through electrospinning technique, and then the physicochemical properties and the cytotoxic effects on dental-derived mesenchymal stem cells were assessed. RESULTS: The nano-scaffolds were successfully prepared with a mono-dispersed nano-scale diameter (102±0.10 nm), negative surface charge (-20±0.17 mV), and uniform network-shaped morphology. The mesenchymal stem cells derived from the human dental pulp stem cells (hDPSC) with gelatin-hydroxyapatite nano-fibers showed that the prepared scaffolds had a significant proliferative effect. Besides, the applied method can be used to prepare fiber-based structures via other polymeric materials. CONCLUSION: The incorporation of different materials to decrease the degradation rate of the fibers can match the speed of tissue regeneration. In this case, the prepared nano-fibers can be applied as a membrane biomaterial. |
format | Online Article Text |
id | pubmed-9327463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-93274632022-08-01 Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells Sharifi, Simin Samiei, Mohammad Dalir Abdolahinia, Elaheh Khalilov, Rovshan Shahi, Shahriar Maleki Dizaj, Solmaz J Adv Periodontol Implant Dent Research Article BACKGROUND: Periodontitis can lead to progressive destruction of periodontal tissues supporting the tooth. Developing biomaterials for tissue engineering has noticeably improved the existing treatment options. The present study investigated the gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR). METHODS: The scaffolds were prepared through electrospinning technique, and then the physicochemical properties and the cytotoxic effects on dental-derived mesenchymal stem cells were assessed. RESULTS: The nano-scaffolds were successfully prepared with a mono-dispersed nano-scale diameter (102±0.10 nm), negative surface charge (-20±0.17 mV), and uniform network-shaped morphology. The mesenchymal stem cells derived from the human dental pulp stem cells (hDPSC) with gelatin-hydroxyapatite nano-fibers showed that the prepared scaffolds had a significant proliferative effect. Besides, the applied method can be used to prepare fiber-based structures via other polymeric materials. CONCLUSION: The incorporation of different materials to decrease the degradation rate of the fibers can match the speed of tissue regeneration. In this case, the prepared nano-fibers can be applied as a membrane biomaterial. Tabriz University of Medical Sciences 2020-04-08 /pmc/articles/PMC9327463/ /pubmed/35919304 http://dx.doi.org/10.34172/japid.2020.001 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sharifi, Simin Samiei, Mohammad Dalir Abdolahinia, Elaheh Khalilov, Rovshan Shahi, Shahriar Maleki Dizaj, Solmaz Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
title | Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
title_full | Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
title_fullStr | Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
title_full_unstemmed | Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
title_short | Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
title_sort | gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (gtr): preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327463/ https://www.ncbi.nlm.nih.gov/pubmed/35919304 http://dx.doi.org/10.34172/japid.2020.001 |
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