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Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+
BACKGROUND: This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC). CASE: An 8‐color MFC combination of antibodies allowed to identify neopla...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327670/ https://www.ncbi.nlm.nih.gov/pubmed/34382381 http://dx.doi.org/10.1002/cnr2.1526 |
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author | Canellas, Maria Clara Bruno‐Riscarolli, Enrico Ferreira‐Facio, Cristiane S. Lopes‐Alves, Daiana V. Botafogo, Vitor D. Sutter, Deborah Pontes, Roberia M. Land, Marcelo G. P. Bedran Milito, Cristiane da Costa, Elaine Sobral |
author_facet | Canellas, Maria Clara Bruno‐Riscarolli, Enrico Ferreira‐Facio, Cristiane S. Lopes‐Alves, Daiana V. Botafogo, Vitor D. Sutter, Deborah Pontes, Roberia M. Land, Marcelo G. P. Bedran Milito, Cristiane da Costa, Elaine Sobral |
author_sort | Canellas, Maria Clara |
collection | PubMed |
description | BACKGROUND: This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC). CASE: An 8‐color MFC combination of antibodies allowed to identify neoplastic cells in concentrations until 0.02% during minimal residual disease (MRD) monitoring. Immunophenotypic shifts occurred in key markers as CD30, CD7, CD2, and CD5, however neoplastic cells were clearly discriminated from normal populations. CONCLUSION: MFC can be a useful tool for ALCL diagnosis and MRD monitoring and may support therapeutic decisions. |
format | Online Article Text |
id | pubmed-9327670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93276702022-07-30 Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ Canellas, Maria Clara Bruno‐Riscarolli, Enrico Ferreira‐Facio, Cristiane S. Lopes‐Alves, Daiana V. Botafogo, Vitor D. Sutter, Deborah Pontes, Roberia M. Land, Marcelo G. P. Bedran Milito, Cristiane da Costa, Elaine Sobral Cancer Rep (Hoboken) Case Reports BACKGROUND: This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC). CASE: An 8‐color MFC combination of antibodies allowed to identify neoplastic cells in concentrations until 0.02% during minimal residual disease (MRD) monitoring. Immunophenotypic shifts occurred in key markers as CD30, CD7, CD2, and CD5, however neoplastic cells were clearly discriminated from normal populations. CONCLUSION: MFC can be a useful tool for ALCL diagnosis and MRD monitoring and may support therapeutic decisions. John Wiley and Sons Inc. 2021-08-11 /pmc/articles/PMC9327670/ /pubmed/34382381 http://dx.doi.org/10.1002/cnr2.1526 Text en © 2021 The Authors. Cancer Reports published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Case Reports Canellas, Maria Clara Bruno‐Riscarolli, Enrico Ferreira‐Facio, Cristiane S. Lopes‐Alves, Daiana V. Botafogo, Vitor D. Sutter, Deborah Pontes, Roberia M. Land, Marcelo G. P. Bedran Milito, Cristiane da Costa, Elaine Sobral Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
title | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
title_full | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
title_fullStr | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
title_full_unstemmed | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
title_short | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
title_sort | immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma alk+ |
topic | Case Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327670/ https://www.ncbi.nlm.nih.gov/pubmed/34382381 http://dx.doi.org/10.1002/cnr2.1526 |
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