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High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning

Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. E...

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Autores principales: Getz, Angela M., Ducros, Mathieu, Breillat, Christelle, Lampin-Saint-Amaux, Aurélie, Daburon, Sophie, François, Urielle, Nowacka, Agata, Fernández-Monreal, Mónica, Hosy, Eric, Lanore, Frédéric, Zieger, Hanna L., Sainlos, Matthieu, Humeau, Yann, Choquet, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328687/
https://www.ncbi.nlm.nih.gov/pubmed/35895810
http://dx.doi.org/10.1126/sciadv.abm5298
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author Getz, Angela M.
Ducros, Mathieu
Breillat, Christelle
Lampin-Saint-Amaux, Aurélie
Daburon, Sophie
François, Urielle
Nowacka, Agata
Fernández-Monreal, Mónica
Hosy, Eric
Lanore, Frédéric
Zieger, Hanna L.
Sainlos, Matthieu
Humeau, Yann
Choquet, Daniel
author_facet Getz, Angela M.
Ducros, Mathieu
Breillat, Christelle
Lampin-Saint-Amaux, Aurélie
Daburon, Sophie
François, Urielle
Nowacka, Agata
Fernández-Monreal, Mónica
Hosy, Eric
Lanore, Frédéric
Zieger, Hanna L.
Sainlos, Matthieu
Humeau, Yann
Choquet, Daniel
author_sort Getz, Angela M.
collection PubMed
description Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. Exploiting AMPA-type glutamate receptors (AMPARs) as a model, we generated a knock-in mouse expressing the biotin acceptor peptide (AP) tag on the GluA2 extracellular N-terminal. Cell-specific introduction of biotin ligase allows the use of monovalent or tetravalent avidin variants to respectively monitor or manipulate the surface mobility of endogenous AMPAR containing biotinylated AP–GluA2 in neuronal subsets. AMPAR immobilization precluded the expression of long-term potentiation and formation of contextual fear memory, allowing target-specific control of the expression of synaptic plasticity and animal behavior. The AP tag knock-in model offers unprecedented access to resolve and control the spatiotemporal dynamics of endogenous receptors, and opens new avenues to study the molecular mechanisms of synaptic plasticity and learning.
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spelling pubmed-93286872022-08-09 High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning Getz, Angela M. Ducros, Mathieu Breillat, Christelle Lampin-Saint-Amaux, Aurélie Daburon, Sophie François, Urielle Nowacka, Agata Fernández-Monreal, Mónica Hosy, Eric Lanore, Frédéric Zieger, Hanna L. Sainlos, Matthieu Humeau, Yann Choquet, Daniel Sci Adv Neuroscience Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. Exploiting AMPA-type glutamate receptors (AMPARs) as a model, we generated a knock-in mouse expressing the biotin acceptor peptide (AP) tag on the GluA2 extracellular N-terminal. Cell-specific introduction of biotin ligase allows the use of monovalent or tetravalent avidin variants to respectively monitor or manipulate the surface mobility of endogenous AMPAR containing biotinylated AP–GluA2 in neuronal subsets. AMPAR immobilization precluded the expression of long-term potentiation and formation of contextual fear memory, allowing target-specific control of the expression of synaptic plasticity and animal behavior. The AP tag knock-in model offers unprecedented access to resolve and control the spatiotemporal dynamics of endogenous receptors, and opens new avenues to study the molecular mechanisms of synaptic plasticity and learning. American Association for the Advancement of Science 2022-07-27 /pmc/articles/PMC9328687/ /pubmed/35895810 http://dx.doi.org/10.1126/sciadv.abm5298 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Neuroscience
Getz, Angela M.
Ducros, Mathieu
Breillat, Christelle
Lampin-Saint-Amaux, Aurélie
Daburon, Sophie
François, Urielle
Nowacka, Agata
Fernández-Monreal, Mónica
Hosy, Eric
Lanore, Frédéric
Zieger, Hanna L.
Sainlos, Matthieu
Humeau, Yann
Choquet, Daniel
High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
title High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
title_full High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
title_fullStr High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
title_full_unstemmed High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
title_short High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
title_sort high-resolution imaging and manipulation of endogenous ampa receptor surface mobility during synaptic plasticity and learning
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328687/
https://www.ncbi.nlm.nih.gov/pubmed/35895810
http://dx.doi.org/10.1126/sciadv.abm5298
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