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High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. E...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328687/ https://www.ncbi.nlm.nih.gov/pubmed/35895810 http://dx.doi.org/10.1126/sciadv.abm5298 |
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author | Getz, Angela M. Ducros, Mathieu Breillat, Christelle Lampin-Saint-Amaux, Aurélie Daburon, Sophie François, Urielle Nowacka, Agata Fernández-Monreal, Mónica Hosy, Eric Lanore, Frédéric Zieger, Hanna L. Sainlos, Matthieu Humeau, Yann Choquet, Daniel |
author_facet | Getz, Angela M. Ducros, Mathieu Breillat, Christelle Lampin-Saint-Amaux, Aurélie Daburon, Sophie François, Urielle Nowacka, Agata Fernández-Monreal, Mónica Hosy, Eric Lanore, Frédéric Zieger, Hanna L. Sainlos, Matthieu Humeau, Yann Choquet, Daniel |
author_sort | Getz, Angela M. |
collection | PubMed |
description | Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. Exploiting AMPA-type glutamate receptors (AMPARs) as a model, we generated a knock-in mouse expressing the biotin acceptor peptide (AP) tag on the GluA2 extracellular N-terminal. Cell-specific introduction of biotin ligase allows the use of monovalent or tetravalent avidin variants to respectively monitor or manipulate the surface mobility of endogenous AMPAR containing biotinylated AP–GluA2 in neuronal subsets. AMPAR immobilization precluded the expression of long-term potentiation and formation of contextual fear memory, allowing target-specific control of the expression of synaptic plasticity and animal behavior. The AP tag knock-in model offers unprecedented access to resolve and control the spatiotemporal dynamics of endogenous receptors, and opens new avenues to study the molecular mechanisms of synaptic plasticity and learning. |
format | Online Article Text |
id | pubmed-9328687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-93286872022-08-09 High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning Getz, Angela M. Ducros, Mathieu Breillat, Christelle Lampin-Saint-Amaux, Aurélie Daburon, Sophie François, Urielle Nowacka, Agata Fernández-Monreal, Mónica Hosy, Eric Lanore, Frédéric Zieger, Hanna L. Sainlos, Matthieu Humeau, Yann Choquet, Daniel Sci Adv Neuroscience Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. Exploiting AMPA-type glutamate receptors (AMPARs) as a model, we generated a knock-in mouse expressing the biotin acceptor peptide (AP) tag on the GluA2 extracellular N-terminal. Cell-specific introduction of biotin ligase allows the use of monovalent or tetravalent avidin variants to respectively monitor or manipulate the surface mobility of endogenous AMPAR containing biotinylated AP–GluA2 in neuronal subsets. AMPAR immobilization precluded the expression of long-term potentiation and formation of contextual fear memory, allowing target-specific control of the expression of synaptic plasticity and animal behavior. The AP tag knock-in model offers unprecedented access to resolve and control the spatiotemporal dynamics of endogenous receptors, and opens new avenues to study the molecular mechanisms of synaptic plasticity and learning. American Association for the Advancement of Science 2022-07-27 /pmc/articles/PMC9328687/ /pubmed/35895810 http://dx.doi.org/10.1126/sciadv.abm5298 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Neuroscience Getz, Angela M. Ducros, Mathieu Breillat, Christelle Lampin-Saint-Amaux, Aurélie Daburon, Sophie François, Urielle Nowacka, Agata Fernández-Monreal, Mónica Hosy, Eric Lanore, Frédéric Zieger, Hanna L. Sainlos, Matthieu Humeau, Yann Choquet, Daniel High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning |
title | High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning |
title_full | High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning |
title_fullStr | High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning |
title_full_unstemmed | High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning |
title_short | High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning |
title_sort | high-resolution imaging and manipulation of endogenous ampa receptor surface mobility during synaptic plasticity and learning |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328687/ https://www.ncbi.nlm.nih.gov/pubmed/35895810 http://dx.doi.org/10.1126/sciadv.abm5298 |
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