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Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota
Cepharanthine (CEP) is an active alkaloid isolated from Stephania Cepharantha Hayata. It is reported that the anti‐inflammatory properties of CEP could be employed to treat a variety of diseases. In this study, we first found that CEP ameliorates ulcerative colitis (UC) induced by DSS. The effect of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328732/ https://www.ncbi.nlm.nih.gov/pubmed/35439340 http://dx.doi.org/10.1111/1751-7915.14059 |
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author | Wang, Hong‐Gang Zhang, Min‐Na Wen, Xin He, Le Zhang, Meng‐Hui Zhang, Jia‐Ling Yang, Xiao‐Zhong |
author_facet | Wang, Hong‐Gang Zhang, Min‐Na Wen, Xin He, Le Zhang, Meng‐Hui Zhang, Jia‐Ling Yang, Xiao‐Zhong |
author_sort | Wang, Hong‐Gang |
collection | PubMed |
description | Cepharanthine (CEP) is an active alkaloid isolated from Stephania Cepharantha Hayata. It is reported that the anti‐inflammatory properties of CEP could be employed to treat a variety of diseases. In this study, we first found that CEP ameliorates ulcerative colitis (UC) induced by DSS. The effect of CEP on gut microbiota was further evaluated by 16S rRNA gene sequencing, antibiotic pretreatment and faecal microbiota transplantation (FMT). Results showed that the abundances of gut microbiota, such as Romboutsia, Turicibacter and Escherichia‐Shigella (especially Romboutsia), were significantly reduced after CEP treatment. Additionally, we explored the mechanisms of CEP by a strategy integrating transcriptomics with network pharmacology. The transcriptome data confirmed that CEP functioned through cytokine and cytokine receptor pathways. The expression levels of 10 pro‐inflammatory hub genes (such as CXCL1, CXCL9, CCL7) were positively correlated with the abundance of Romboutsia. Our data identified Romboutsia as a potential pathobiont in UC. Collectively, we confirmed that CEP relieved colon inflammation by modulating gut microbiota and pro‐inflammatory cytokine expression. CEP can be adopted to design novel effective therapeutic strategies for UC. |
format | Online Article Text |
id | pubmed-9328732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93287322022-07-30 Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota Wang, Hong‐Gang Zhang, Min‐Na Wen, Xin He, Le Zhang, Meng‐Hui Zhang, Jia‐Ling Yang, Xiao‐Zhong Microb Biotechnol Research Articles Cepharanthine (CEP) is an active alkaloid isolated from Stephania Cepharantha Hayata. It is reported that the anti‐inflammatory properties of CEP could be employed to treat a variety of diseases. In this study, we first found that CEP ameliorates ulcerative colitis (UC) induced by DSS. The effect of CEP on gut microbiota was further evaluated by 16S rRNA gene sequencing, antibiotic pretreatment and faecal microbiota transplantation (FMT). Results showed that the abundances of gut microbiota, such as Romboutsia, Turicibacter and Escherichia‐Shigella (especially Romboutsia), were significantly reduced after CEP treatment. Additionally, we explored the mechanisms of CEP by a strategy integrating transcriptomics with network pharmacology. The transcriptome data confirmed that CEP functioned through cytokine and cytokine receptor pathways. The expression levels of 10 pro‐inflammatory hub genes (such as CXCL1, CXCL9, CCL7) were positively correlated with the abundance of Romboutsia. Our data identified Romboutsia as a potential pathobiont in UC. Collectively, we confirmed that CEP relieved colon inflammation by modulating gut microbiota and pro‐inflammatory cytokine expression. CEP can be adopted to design novel effective therapeutic strategies for UC. John Wiley and Sons Inc. 2022-04-19 /pmc/articles/PMC9328732/ /pubmed/35439340 http://dx.doi.org/10.1111/1751-7915.14059 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Wang, Hong‐Gang Zhang, Min‐Na Wen, Xin He, Le Zhang, Meng‐Hui Zhang, Jia‐Ling Yang, Xiao‐Zhong Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
title | Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
title_full | Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
title_fullStr | Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
title_full_unstemmed | Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
title_short | Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
title_sort | cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328732/ https://www.ncbi.nlm.nih.gov/pubmed/35439340 http://dx.doi.org/10.1111/1751-7915.14059 |
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