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Assessment of antibacterial drug residues in milk for consumption in Kosovo

The objective of this study was to assess the occurrence of drug residues in the raw milk collected from individual farms and milk collection points during 2009–2010 in six different major regions of Kosovo (Prishtinë, Gjilan, Mitrovicë, Pejë, Gjakovë, Prizren). In the present study, a total of 1734...

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Autores principales: Rama, Adem, Lucatello, Lorena, Benetti, Cristiana, Galina, Guglielmo, Bajraktari, Drita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328814/
https://www.ncbi.nlm.nih.gov/pubmed/28911638
http://dx.doi.org/10.1016/j.jfda.2016.07.007
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author Rama, Adem
Lucatello, Lorena
Benetti, Cristiana
Galina, Guglielmo
Bajraktari, Drita
author_facet Rama, Adem
Lucatello, Lorena
Benetti, Cristiana
Galina, Guglielmo
Bajraktari, Drita
author_sort Rama, Adem
collection PubMed
description The objective of this study was to assess the occurrence of drug residues in the raw milk collected from individual farms and milk collection points during 2009–2010 in six different major regions of Kosovo (Prishtinë, Gjilan, Mitrovicë, Pejë, Gjakovë, Prizren). In the present study, a total of 1734 raw milk samples were collected, and qualitatively screened with two different tests, the Delvotest SP assay and an enzyme-linked receptor-binding assay (SNAP). Overall, 106 (6.11%) out of 1734 samples examined with Delvotest SP contained possible drug residues (5.12% and 7.51% of samples from 2009 and 2010, respectively). All suspect samples were further analyzed by three distinct enzyme-linked receptor-binding assays specific for β-lactams (new β-lactam test), tetracyclines (SNAP tetracycline test), and sulfonamides (SNAP sulfamethazine test). Only the new SNAP βlactam test detected residues in 40 out of 52 samples in 2009 and 54 out of 54 suspect samples in 2010. A confirmatory method based on liquid chromatography-tandem mass spectrometry was used to confirm the presence of β-lactam drug residues in samples detected by the enzyme-linked receptor-binding assay. Amoxicillin, penicillin G, and cloxacillin were the most frequently detected residues and were in a concentration range between 2.1 μg/kg and 1973 μg/kg. Seventeen of the positive samples exceeded the maximum residue levels for one or more β-lactam drug. The highest number of positive milk samples came from the Pejë Region (58.8%) and Gjakovë Region (23.5%), and the lowest number of positive samples originated from Gjilan (5.88%), with no positive samples detected in two regions, Mitrovicë and Prizren.
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spelling pubmed-93288142022-08-09 Assessment of antibacterial drug residues in milk for consumption in Kosovo Rama, Adem Lucatello, Lorena Benetti, Cristiana Galina, Guglielmo Bajraktari, Drita J Food Drug Anal Original Article The objective of this study was to assess the occurrence of drug residues in the raw milk collected from individual farms and milk collection points during 2009–2010 in six different major regions of Kosovo (Prishtinë, Gjilan, Mitrovicë, Pejë, Gjakovë, Prizren). In the present study, a total of 1734 raw milk samples were collected, and qualitatively screened with two different tests, the Delvotest SP assay and an enzyme-linked receptor-binding assay (SNAP). Overall, 106 (6.11%) out of 1734 samples examined with Delvotest SP contained possible drug residues (5.12% and 7.51% of samples from 2009 and 2010, respectively). All suspect samples were further analyzed by three distinct enzyme-linked receptor-binding assays specific for β-lactams (new β-lactam test), tetracyclines (SNAP tetracycline test), and sulfonamides (SNAP sulfamethazine test). Only the new SNAP βlactam test detected residues in 40 out of 52 samples in 2009 and 54 out of 54 suspect samples in 2010. A confirmatory method based on liquid chromatography-tandem mass spectrometry was used to confirm the presence of β-lactam drug residues in samples detected by the enzyme-linked receptor-binding assay. Amoxicillin, penicillin G, and cloxacillin were the most frequently detected residues and were in a concentration range between 2.1 μg/kg and 1973 μg/kg. Seventeen of the positive samples exceeded the maximum residue levels for one or more β-lactam drug. The highest number of positive milk samples came from the Pejë Region (58.8%) and Gjakovë Region (23.5%), and the lowest number of positive samples originated from Gjilan (5.88%), with no positive samples detected in two regions, Mitrovicë and Prizren. Taiwan Food and Drug Administration 2016-11-05 /pmc/articles/PMC9328814/ /pubmed/28911638 http://dx.doi.org/10.1016/j.jfda.2016.07.007 Text en © 2017 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Rama, Adem
Lucatello, Lorena
Benetti, Cristiana
Galina, Guglielmo
Bajraktari, Drita
Assessment of antibacterial drug residues in milk for consumption in Kosovo
title Assessment of antibacterial drug residues in milk for consumption in Kosovo
title_full Assessment of antibacterial drug residues in milk for consumption in Kosovo
title_fullStr Assessment of antibacterial drug residues in milk for consumption in Kosovo
title_full_unstemmed Assessment of antibacterial drug residues in milk for consumption in Kosovo
title_short Assessment of antibacterial drug residues in milk for consumption in Kosovo
title_sort assessment of antibacterial drug residues in milk for consumption in kosovo
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328814/
https://www.ncbi.nlm.nih.gov/pubmed/28911638
http://dx.doi.org/10.1016/j.jfda.2016.07.007
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