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ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy

BACKGROUND: In the early stage of nerve injury, damaged tissue is cleared by autophagy. ADSCs can promote nerve axon regeneration. However, the microenvironment of the injury was changed, and ADSCs are easily apoptotic after transplantation. Mel plays a role in the apoptosis, proliferation, and diff...

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Autores principales: Zhang, Ziqiang, Zhang, Mengyu, Zhang, Zhixiang, Sun, Yingying, Wang, Jiajia, Chang, Chenhao, Zhu, Xinyan, Li, Monan, Liu, Yumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329031/
https://www.ncbi.nlm.nih.gov/pubmed/35910940
http://dx.doi.org/10.1155/2022/5861553
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author Zhang, Ziqiang
Zhang, Mengyu
Zhang, Zhixiang
Sun, Yingying
Wang, Jiajia
Chang, Chenhao
Zhu, Xinyan
Li, Monan
Liu, Yumei
author_facet Zhang, Ziqiang
Zhang, Mengyu
Zhang, Zhixiang
Sun, Yingying
Wang, Jiajia
Chang, Chenhao
Zhu, Xinyan
Li, Monan
Liu, Yumei
author_sort Zhang, Ziqiang
collection PubMed
description BACKGROUND: In the early stage of nerve injury, damaged tissue is cleared by autophagy. ADSCs can promote nerve axon regeneration. However, the microenvironment of the injury was changed, and ADSCs are easily apoptotic after transplantation. Mel plays a role in the apoptosis, proliferation, and differentiation of ADSCs. Therefore, we investigated whether Mel combined with ADSCs promoted peripheral nerve regeneration by enhancing early autophagy of injured nerves. MATERIALS AND METHODS: SD rats were randomly split into the control group, model group, Mel group, ADSCs group, ADSCs + Mel group, and 3-MA group. On day 7, autophagy was observed and gait was detected on days 7, 14, 21, and 28. On the 28th day, the sciatic nerve of rats' renewal was detected. RESULTS: After 1 w, compare with the model group, the number of autophagosomes and lysosomes and the expressions of protein of LC3-II/LC3-I and Beclin-1 in the ADSCs + Mel group were prominently increased, while the 3-MA group was significantly decreased. After 4 w, the function of the sciatic nerve in ADSCs + Mel was similar to that in the control group. Compared with the model group, the ADSCs + Mel group significantly increased myelin regeneration and the number of motor neurons and reduced gastrocnemius atrophy. CONCLUSIONS: It was confirmed that ADSCs combined with Mel could promote sciatic nerve regeneration in rats by changing the early autophagy activity of the injured sciatic nerve.
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spelling pubmed-93290312022-07-28 ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy Zhang, Ziqiang Zhang, Mengyu Zhang, Zhixiang Sun, Yingying Wang, Jiajia Chang, Chenhao Zhu, Xinyan Li, Monan Liu, Yumei Int J Endocrinol Research Article BACKGROUND: In the early stage of nerve injury, damaged tissue is cleared by autophagy. ADSCs can promote nerve axon regeneration. However, the microenvironment of the injury was changed, and ADSCs are easily apoptotic after transplantation. Mel plays a role in the apoptosis, proliferation, and differentiation of ADSCs. Therefore, we investigated whether Mel combined with ADSCs promoted peripheral nerve regeneration by enhancing early autophagy of injured nerves. MATERIALS AND METHODS: SD rats were randomly split into the control group, model group, Mel group, ADSCs group, ADSCs + Mel group, and 3-MA group. On day 7, autophagy was observed and gait was detected on days 7, 14, 21, and 28. On the 28th day, the sciatic nerve of rats' renewal was detected. RESULTS: After 1 w, compare with the model group, the number of autophagosomes and lysosomes and the expressions of protein of LC3-II/LC3-I and Beclin-1 in the ADSCs + Mel group were prominently increased, while the 3-MA group was significantly decreased. After 4 w, the function of the sciatic nerve in ADSCs + Mel was similar to that in the control group. Compared with the model group, the ADSCs + Mel group significantly increased myelin regeneration and the number of motor neurons and reduced gastrocnemius atrophy. CONCLUSIONS: It was confirmed that ADSCs combined with Mel could promote sciatic nerve regeneration in rats by changing the early autophagy activity of the injured sciatic nerve. Hindawi 2022-07-20 /pmc/articles/PMC9329031/ /pubmed/35910940 http://dx.doi.org/10.1155/2022/5861553 Text en Copyright © 2022 Ziqiang Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Ziqiang
Zhang, Mengyu
Zhang, Zhixiang
Sun, Yingying
Wang, Jiajia
Chang, Chenhao
Zhu, Xinyan
Li, Monan
Liu, Yumei
ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy
title ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy
title_full ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy
title_fullStr ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy
title_full_unstemmed ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy
title_short ADSCs Combined with Melatonin Promote Peripheral Nerve Regeneration through Autophagy
title_sort adscs combined with melatonin promote peripheral nerve regeneration through autophagy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329031/
https://www.ncbi.nlm.nih.gov/pubmed/35910940
http://dx.doi.org/10.1155/2022/5861553
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