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Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars
Tea is a widely consumed beverage prepared using the fresh leaves of Camellia sinensis L. Tea plants are classified into small- and large-leaf varieties. Polyphenol oxidase (PPO), a crucial enzyme in tea manufacturing, catalyzes essential phenolic metabolites into different derivatives. To compare t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329367/ https://www.ncbi.nlm.nih.gov/pubmed/35896690 http://dx.doi.org/10.1038/s41598-022-17184-1 |
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author | Chen, Chung‑Tse Yang, Chin-Ying Tzen, Jason T. C. |
author_facet | Chen, Chung‑Tse Yang, Chin-Ying Tzen, Jason T. C. |
author_sort | Chen, Chung‑Tse |
collection | PubMed |
description | Tea is a widely consumed beverage prepared using the fresh leaves of Camellia sinensis L. Tea plants are classified into small- and large-leaf varieties. Polyphenol oxidase (PPO), a crucial enzyme in tea manufacturing, catalyzes essential phenolic metabolites into different derivatives. To compare the molecular characteristics of CsPPO between cultivars, we cloned the full-length sequence of CsPPO cDNA from four representative tea cultivars in Taiwan. Amino acid sequence alignment analyses indicated that CsPPO is highly conserved. PPO exhibited similar enzymatic activity in different tea cultivars. Quantitative real-time polymerase chain reaction revealed no significant differences in the CsPPO transcript level between the small- and large-leaf varieties. However, tea harvested in summer and from low-altitude areas had a higher CsPPO transcript level than that harvested in winter and from high-altitude areas. Regulation of CsPPO by temperature was more significant in the small-leaf variety than in the large-leaf variety. The content of flavonoids and the expression of chalcone synthase, anthocyanidin synthase, and anthocyanidin reductase genes involved in the tea flavonoid biosynthesis pathway were higher in the large-leaf than in the small-leaf varieties, suggesting that the large-leaf variety had a higher antioxidative capacity than did the small-leaf variety. Our study compared the molecular properties of CsPPO between two tea varieties and clarified the physiological role of PPO in tea. |
format | Online Article Text |
id | pubmed-9329367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93293672022-07-29 Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars Chen, Chung‑Tse Yang, Chin-Ying Tzen, Jason T. C. Sci Rep Article Tea is a widely consumed beverage prepared using the fresh leaves of Camellia sinensis L. Tea plants are classified into small- and large-leaf varieties. Polyphenol oxidase (PPO), a crucial enzyme in tea manufacturing, catalyzes essential phenolic metabolites into different derivatives. To compare the molecular characteristics of CsPPO between cultivars, we cloned the full-length sequence of CsPPO cDNA from four representative tea cultivars in Taiwan. Amino acid sequence alignment analyses indicated that CsPPO is highly conserved. PPO exhibited similar enzymatic activity in different tea cultivars. Quantitative real-time polymerase chain reaction revealed no significant differences in the CsPPO transcript level between the small- and large-leaf varieties. However, tea harvested in summer and from low-altitude areas had a higher CsPPO transcript level than that harvested in winter and from high-altitude areas. Regulation of CsPPO by temperature was more significant in the small-leaf variety than in the large-leaf variety. The content of flavonoids and the expression of chalcone synthase, anthocyanidin synthase, and anthocyanidin reductase genes involved in the tea flavonoid biosynthesis pathway were higher in the large-leaf than in the small-leaf varieties, suggesting that the large-leaf variety had a higher antioxidative capacity than did the small-leaf variety. Our study compared the molecular properties of CsPPO between two tea varieties and clarified the physiological role of PPO in tea. Nature Publishing Group UK 2022-07-27 /pmc/articles/PMC9329367/ /pubmed/35896690 http://dx.doi.org/10.1038/s41598-022-17184-1 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chen, Chung‑Tse Yang, Chin-Ying Tzen, Jason T. C. Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
title | Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
title_full | Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
title_fullStr | Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
title_full_unstemmed | Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
title_short | Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
title_sort | molecular characterization of polyphenol oxidase between small and large leaf tea cultivars |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329367/ https://www.ncbi.nlm.nih.gov/pubmed/35896690 http://dx.doi.org/10.1038/s41598-022-17184-1 |
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