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The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system

The combination of trimeric form of the light-harvesting complex II (LHCII(3)), a porous graphite electrode (GE), and the application of phenyl-p-benzoquinone (PPBQ), the quinone derivative, allow the construction of a new type of biohybrid photoactive system. The Chl fluorescence decay and voltamme...

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Detalles Bibliográficos
Autores principales: Łazicka, Magdalena, Palińska-Saadi, Adriana, Piotrowska, Paulina, Paterczyk, Bohdan, Mazur, Radosław, Maj-Żurawska, Magdalena, Garstka, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329374/
https://www.ncbi.nlm.nih.gov/pubmed/35896789
http://dx.doi.org/10.1038/s41598-022-16892-y
Descripción
Sumario:The combination of trimeric form of the light-harvesting complex II (LHCII(3)), a porous graphite electrode (GE), and the application of phenyl-p-benzoquinone (PPBQ), the quinone derivative, allow the construction of a new type of biohybrid photoactive system. The Chl fluorescence decay and voltammetric analyzes revealed that PPBQ impacts LHCII(3) proportionally to accessible quenching sites and that PPBQ forms redox complexes with Chl in both ground and excited states. As a result, photocurrent generation is directly dependent on PPBQ-induced quenching of Chl fluorescence. Since PPBQ also undergoes photoactivation, the action of GE-LHCII(3)-PPBQ depends on the mutual coupling of LHCII(3) and PPBQ photocycles. The GE-LHCII(3)-PPBQ generates a photocurrent of up to 4.5 µA and exhibits considerable stability during operation. The three-dimensional arrangement of graphite scraps in GE builds an active electrode surface and stabilizes LHCII(3) in its native form in low-density multilayers. The results indicate the future usability of such designed photoactive device.