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UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy

Ultra-violet (UV) light has still a limited scope in optical microscopy despite its potential advantages over visible light in terms of optical resolution and of interaction with a wide variety of biological molecules. The main challenge is to control in a robust, compact and cost-effective way UV l...

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Autores principales: Lin, Chupao, Peñaranda, Juan Santo Domingo, Dendooven, Jolien, Detavernier, Christophe, Schaubroeck, David, Boon, Nico, Baets, Roel, Le Thomas, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329385/
https://www.ncbi.nlm.nih.gov/pubmed/35896536
http://dx.doi.org/10.1038/s41467-022-31989-8
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author Lin, Chupao
Peñaranda, Juan Santo Domingo
Dendooven, Jolien
Detavernier, Christophe
Schaubroeck, David
Boon, Nico
Baets, Roel
Le Thomas, Nicolas
author_facet Lin, Chupao
Peñaranda, Juan Santo Domingo
Dendooven, Jolien
Detavernier, Christophe
Schaubroeck, David
Boon, Nico
Baets, Roel
Le Thomas, Nicolas
author_sort Lin, Chupao
collection PubMed
description Ultra-violet (UV) light has still a limited scope in optical microscopy despite its potential advantages over visible light in terms of optical resolution and of interaction with a wide variety of biological molecules. The main challenge is to control in a robust, compact and cost-effective way UV light beams at the level of a single optical spatial mode and concomitantly to minimize the light propagation loss. To tackle this challenge, we present here photonic integrated circuits made of aluminum oxide thin layers that are compatible with both UV light and high-volume manufacturing. These photonic circuits designed at a wavelength of 360 nm enable super-resolved structured illumination microscopy with conventional wide-field microscopes and without modifying the usual protocol for handling the object to be imaged. As a biological application, we show that our UV photonic chips enable to image the autofluorescence of yeast cells and reveal features unresolved with standard wide-field microscopy.
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spelling pubmed-93293852022-07-29 UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy Lin, Chupao Peñaranda, Juan Santo Domingo Dendooven, Jolien Detavernier, Christophe Schaubroeck, David Boon, Nico Baets, Roel Le Thomas, Nicolas Nat Commun Article Ultra-violet (UV) light has still a limited scope in optical microscopy despite its potential advantages over visible light in terms of optical resolution and of interaction with a wide variety of biological molecules. The main challenge is to control in a robust, compact and cost-effective way UV light beams at the level of a single optical spatial mode and concomitantly to minimize the light propagation loss. To tackle this challenge, we present here photonic integrated circuits made of aluminum oxide thin layers that are compatible with both UV light and high-volume manufacturing. These photonic circuits designed at a wavelength of 360 nm enable super-resolved structured illumination microscopy with conventional wide-field microscopes and without modifying the usual protocol for handling the object to be imaged. As a biological application, we show that our UV photonic chips enable to image the autofluorescence of yeast cells and reveal features unresolved with standard wide-field microscopy. Nature Publishing Group UK 2022-07-27 /pmc/articles/PMC9329385/ /pubmed/35896536 http://dx.doi.org/10.1038/s41467-022-31989-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lin, Chupao
Peñaranda, Juan Santo Domingo
Dendooven, Jolien
Detavernier, Christophe
Schaubroeck, David
Boon, Nico
Baets, Roel
Le Thomas, Nicolas
UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy
title UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy
title_full UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy
title_fullStr UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy
title_full_unstemmed UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy
title_short UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy
title_sort uv photonic integrated circuits for far-field structured illumination autofluorescence microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329385/
https://www.ncbi.nlm.nih.gov/pubmed/35896536
http://dx.doi.org/10.1038/s41467-022-31989-8
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