Cargando…

Water Droplet-in-Oil Digestion Method for Single-Cell Proteomics

[Image: see text] Recent advances in single-cell proteomics highlight the promise of sensitive analyses in limited cell populations. However, technical challenges remain for sample recovery, throughput, and versatility. Here, we first report a water droplet-in-oil digestion (WinO) method based on ca...

Descripción completa

Detalles Bibliográficos
Autores principales:	Masuda, Takeshi, Inamori, Yuma, Furukawa, Arisu, Yamahiro, Maki, Momosaki, Kazuki, Chang, Chih-Hsiang, Kobayashi, Daiki, Ohguchi, Hiroto, Kawano, Yawara, Ito, Shingo, Araki, Norie, Ong, Shao-En, Ohtsuki, Sumio
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330287/ https://www.ncbi.nlm.nih.gov/pubmed/35817413 http://dx.doi.org/10.1021/acs.analchem.1c05487

Ejemplares similares

Development of a method for isolating brain capillaries from a single neonatal mouse brain and comparison of proteomic profiles between neonatal and adult brain capillaries
por: Hamada, Yudai, et al.
Publicado: (2023)

Diverse Functions of KDM5 in Cancer: Transcriptional Repressor or Activator?
por: Ohguchi, Yasuyo, et al.
Publicado: (2022)

DIS3: The Enigmatic Gene in Multiple Myeloma
por: Ohguchi, Yasuyo, et al.
Publicado: (2023)

Proteomic alterations in the brain and blood–brain barrier during brain Aβ accumulation in an APP knock-in mouse model of Alzheimer’s disease
por: Ito, Shingo, et al.
Publicado: (2023)

Identification of Cell-Surface Proteins Endocytosed by Human Brain Microvascular Endothelial Cells In Vitro
por: Ito, Shingo, et al.
Publicado: (2020)

Reduction in hepatic secondary bile acids caused by short-term antibiotic-induced dysbiosis decreases mouse serum glucose and triglyceride levels
por: Kuno, Takuya, et al.
Publicado: (2018)

In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN5
por: Hatakeyama, Dai, et al.
Publicado: (2021)

1α,25-Dihydroxyvitamin D(3 )enhances cerebral clearance of human amyloid-β peptide(1-40) from mouse brain across the blood-brain barrier
por: Ito, Shingo, et al.
Publicado: (2011)

The biological significance of histone modifiers in multiple myeloma: clinical applications
por: Ohguchi, Hiroto, et al.
Publicado: (2018)

Author Correction: The biological significance of histone modifiers in multiple myeloma: clinical applications
por: Ohguchi, Hiroto, et al.
Publicado: (2021)

Reputation structure in indirect reciprocity under noisy and private assessment
por: Fujimoto, Yuma, et al.
Publicado: (2022)

Evolutionary stability of cooperation in indirect reciprocity under noisy and private assessment
por: Fujimoto, Yuma, et al.
Publicado: (2023)

TogoTable: cross-database annotation system using the Resource Description Framework (RDF) data model
por: Kawano, Shin, et al.
Publicado: (2014)

SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix
por: Fukuda, Masatoshi, et al.
Publicado: (2018)

The combined effect of the T2DM susceptibility genes is an important risk factor for T2DM in non-obese Japanese: a population based case-control study
por: Yamakawa-Kobayashi, Kimiko, et al.
Publicado: (2012)

A gain‐of‐function mutation in microRNA 142 is sufficient to cause the development of T‐cell leukemia in mice
por: Kawano, Shingo, et al.
Publicado: (2023)

Lipid Droplets in the Basal Cistern and Ventricles
por: Maeda, Kengo, et al.
Publicado: (2018)

Observation of liquid–liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy
por: Murakami, Kazuki, et al.
Publicado: (2021)

Case Report: Treatment of Delusions of Theft Based on the Assessment of Photos of Patients' Homes
por: Ishimaru, Daiki, et al.
Publicado: (2022)

SETD1A regulates transcriptional pause release of heme biosynthesis genes in leukemia
por: Hoshii, Takayuki, et al.
Publicado: (2022)

Contactless mass transfer for intra-droplet extraction
por: Asano, Shusaku, et al.
Publicado: (2020)

Proteolytic release of CD44 intracellular domain and its role in the CD44 signaling pathway
por: Okamoto, Isamu, et al.
Publicado: (2001)

jPOSTrepo: an international standard data repository for proteomes
por: Okuda, Shujiro, et al.
Publicado: (2017)

Predicting the splash of a droplet impinging on solid substrates
por: Yonemoto, Yukihiro, et al.
Publicado: (2022)

Suppressing the coffee-ring effect of colloidal droplets by dispersed cellulose nanofibers
por: Ooi, Yuto, et al.
Publicado: (2017)

Role of the Mouse Retinal Photoreceptor Ribbon Synapse in Visual Motion Processing for Optokinetic Responses
por: Sugita, Yuko, et al.
Publicado: (2015)

Diagnosis and Treatment of Three-Rooted Maxillary Premolars
por: Arisu, Hacer Deniz, et al.
Publicado: (2009)

Evaluation of cytochrome P450-based drug metabolism in hemorrhagic shock rats that were transfused with native and an artificial red blood cell preparation, Hemoglobin-vesicles
por: Tokuno, Masahiro, et al.
Publicado: (2020)

Downregulation of ERG and FLI1 expression in endothelial cells triggers endothelial-to-mesenchymal transition
por: Nagai, Nao, et al.
Publicado: (2018)

Modeling of Al and Ga Droplet Nucleation during Droplet Epitaxy or Droplet Etching
por: Heyn, Christian, et al.
Publicado: (2021)

Directional and velocity control of active droplets using a rigid-frame
por: Yamada, Masato, et al.
Publicado: (2019)

Condensation of Satellite Droplets on Lubricant-Cloaked Droplets
por: Ge, Qiaoyu, et al.
Publicado: (2020)

The vortex-driven dynamics of droplets within droplets
por: Tiribocchi, A., et al.
Publicado: (2021)

Creation of an artificial intelligence model for intubation difficulty classification by deep learning (convolutional neural network) using face images: an observational study
por: Hayasaka, Tatsuya, et al.
Publicado: (2021)

Size-Dependent and Property-Independent Passive Microdroplet Sorting by Droplet Transfer on Dot Rails
por: Yoon, Dong Hyun, et al.
Publicado: (2018)

Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption
por: Fujiwara, Shougo, et al.
Publicado: (2020)

The jPOST environment: an integrated proteomics data repository and database
por: Moriya, Yuki, et al.
Publicado: (2019)

Development of a quantitative methylation-specific droplet digital PCR assay for detecting Dickkopf-related protein 3
por: Araki, Kenji, et al.
Publicado: (2022)

Single-molecule characterization of target search dynamics of DNA-binding proteins in DNA-condensed droplets
por: Kamagata, Kiyoto, et al.
Publicado: (2023)

Spatial-Temporal Cellular Bioeffects from Acoustic Droplet Vaporization
por: Fan, Ching-Hsiang, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_mvp4ddc0dnat3jr4tc2lj68cnd