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Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma

CD71(+) erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-o...

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Autores principales: Perik-Zavodskii, Roman, Perik-Zavodskaya, Olga, Shevchenko, Yulia, Denisova, Vera, Nazarov, Kirill, Obleuhova, Irina, Zaitsev, Konstantin, Sennikov, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330402/
https://www.ncbi.nlm.nih.gov/pubmed/35893070
http://dx.doi.org/10.3390/genes13081333
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author Perik-Zavodskii, Roman
Perik-Zavodskaya, Olga
Shevchenko, Yulia
Denisova, Vera
Nazarov, Kirill
Obleuhova, Irina
Zaitsev, Konstantin
Sennikov, Sergey
author_facet Perik-Zavodskii, Roman
Perik-Zavodskaya, Olga
Shevchenko, Yulia
Denisova, Vera
Nazarov, Kirill
Obleuhova, Irina
Zaitsev, Konstantin
Sennikov, Sergey
author_sort Perik-Zavodskii, Roman
collection PubMed
description CD71(+) erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs.
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spelling pubmed-93304022022-07-29 Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma Perik-Zavodskii, Roman Perik-Zavodskaya, Olga Shevchenko, Yulia Denisova, Vera Nazarov, Kirill Obleuhova, Irina Zaitsev, Konstantin Sennikov, Sergey Genes (Basel) Article CD71(+) erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs. MDPI 2022-07-26 /pmc/articles/PMC9330402/ /pubmed/35893070 http://dx.doi.org/10.3390/genes13081333 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perik-Zavodskii, Roman
Perik-Zavodskaya, Olga
Shevchenko, Yulia
Denisova, Vera
Nazarov, Kirill
Obleuhova, Irina
Zaitsev, Konstantin
Sennikov, Sergey
Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
title Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
title_full Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
title_fullStr Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
title_full_unstemmed Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
title_short Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
title_sort immune transcriptome and secretome differ between human cd71(+) erythroid cells from adult bone marrow and fetal liver parenchyma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330402/
https://www.ncbi.nlm.nih.gov/pubmed/35893070
http://dx.doi.org/10.3390/genes13081333
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