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Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
CD71(+) erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330402/ https://www.ncbi.nlm.nih.gov/pubmed/35893070 http://dx.doi.org/10.3390/genes13081333 |
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author | Perik-Zavodskii, Roman Perik-Zavodskaya, Olga Shevchenko, Yulia Denisova, Vera Nazarov, Kirill Obleuhova, Irina Zaitsev, Konstantin Sennikov, Sergey |
author_facet | Perik-Zavodskii, Roman Perik-Zavodskaya, Olga Shevchenko, Yulia Denisova, Vera Nazarov, Kirill Obleuhova, Irina Zaitsev, Konstantin Sennikov, Sergey |
author_sort | Perik-Zavodskii, Roman |
collection | PubMed |
description | CD71(+) erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs. |
format | Online Article Text |
id | pubmed-9330402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93304022022-07-29 Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma Perik-Zavodskii, Roman Perik-Zavodskaya, Olga Shevchenko, Yulia Denisova, Vera Nazarov, Kirill Obleuhova, Irina Zaitsev, Konstantin Sennikov, Sergey Genes (Basel) Article CD71(+) erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs. MDPI 2022-07-26 /pmc/articles/PMC9330402/ /pubmed/35893070 http://dx.doi.org/10.3390/genes13081333 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Perik-Zavodskii, Roman Perik-Zavodskaya, Olga Shevchenko, Yulia Denisova, Vera Nazarov, Kirill Obleuhova, Irina Zaitsev, Konstantin Sennikov, Sergey Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
title | Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
title_full | Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
title_fullStr | Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
title_full_unstemmed | Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
title_short | Immune Transcriptome and Secretome Differ between Human CD71(+) Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
title_sort | immune transcriptome and secretome differ between human cd71(+) erythroid cells from adult bone marrow and fetal liver parenchyma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330402/ https://www.ncbi.nlm.nih.gov/pubmed/35893070 http://dx.doi.org/10.3390/genes13081333 |
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